Biocontrol of Listeria monocytogenes on Fresh-Cut Produce by Treatment with Lytic Bacteriophages and a Bacteriocin

Applied and Environmental Microbiology - Tập 69 Số 8 - Trang 4519-4526 - 2003
Britta Leverentz1, William S. Conway1, Mary J. Camp2, Wojciech J. Janisiewicz3, Tamuna Abuladze4, Meixue Yang4, Robert A. Saftner1, Alexander Sulakvelidze5,4
1Produce Quality and Safety Laboratory
2Biometrical Consulting Service, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705
3Appalachian Fruit Research Station, Agricultural Research Service, U.S. Department of Agriculture, Kearneysville, West Virginia 25430
4Intralytix, Inc., Baltimore, Maryland 21202
5Department of Epidemiology and Preventive Medicine, University of Maryland, Baltimore, Maryland 21201

Tóm tắt

ABSTRACT The fresh-cut produce industry has been the fastest-growing portion of the food retail market during the past 10 years, providing consumers with convenient and nutritious food. However, fresh-cut fruits and vegetables raise food safety concerns, because exposed tissue may be colonized more easily by pathogenic bacteria than intact produce. This is due to the higher availability of nutrients on cut surfaces and the greater potential for contamination because of the increased amount of handling. We found that applied Listeria monocytogenes populations survived and increased only slightly on fresh-cut Red Delicious apples stored at 10°C but increased significantly on fresh-cut honeydew melons stored at 10°C over 7 days. In addition, we examined the effect of lytic, L. monocytogenes -specific phages via two phage application methods, spraying and pipetting, on L. monocytogenes populations in artificially contaminated fresh-cut melons and apples. The phage mixture reduced L. monocytogenes populations by 2.0 to 4.6 log units over the control on honeydew melons. On apples, the reduction was below 0.4 log units. In combination with nisin (a bacteriocin), the phage mixture reduced L. monocytogenes populations by up to 5.7 log units on honeydew melon slices and by up to 2.3 log units on apple slices compared to the control. Nisin alone reduced L. monocytogenes populations by up to 3.2 log units on honeydew melon slices and by up to 2.0 log units on apple slices compared to the control. The phage titer was stable on melon slices, but declined rapidly on apple slices. The spray application of the phage and phage plus nisin reduced the bacterial numbers at least as much as the pipette application. The effectiveness of the phage treatment also depended on the initial concentration of L. monocytogenes .

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Tài liệu tham khảo

Adams M. H. 1959. Bacteriophages. Interscience Publishers New York N.Y.

10.1016/S0163-4453(98)92874-2

Arber W. L. Enquist B. Hohn N. Murray and K. Murray. 1983. Experimental methods for use with Lambda. In R. Hendrix J. Roberts F. Stahl and R. Weisberg (ed.) Lambda II. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

10.1016/S1286-4579(02)01555-1

10.1094/PDIS.2000.84.2.177

10.1128/mr.55.3.476-511.1991

10.4315/0362-028X-61.2.192

Feiss M. and A. Becker. 1983. DNA packaging and cutting. In R. Hendrix J. Roberts F. Stahl and R. Weisberg (ed.) Lambda II. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

Food and Drug Administration/Center for Food Safety and Applied Nutrition. 2001. Direct food substances affirmed as generally recognized as safe p. 462-464.Title 21 of the Code of Federal Regulations—Food and Drugs chapter 1 part 184. Food and Drug Administration Department of Health and Human Services Washington D.C.

10.1046/j.1365-2621.1999.00253.x

Gould G. W. 1996. Industry perspectives on the use of natural antimicrobials and inhibitors for food applications. J. Food Prot. 1996 (Suppl.): 82-86.

10.4315/0362-028X-49.2.104

10.1016/0168-1605(90)90080-O

10.4315/0362-028X-65.5.861

10.4315/0362-028X-64.11.1730

10.1016/0168-1605(94)00036-6

Jack R. W. G. Bierbaum and H.-G. Sahl. 1998. Biological activities p. 157-190. Lantibiotics and related peptides. Springer-Verlag and Landes Bioscience Georgetown Tex.

10.4315/0362-028X-65.4.603

10.1016/0963-9969(92)90158-2

Leverentz B. W. J. Janisiewicz and W. S. Conway. 2002. Biological control of minimally processed fruits and vegetables p. 319-332. In J. S. Novak G. M. Sapers and V. K. Juneja (ed.) Microbial safety of minimally processed foods. CRC Press Boca Raton Fla.

10.4315/0362-028X-64.8.1116

10.1128/AEM.68.12.5904-5910.2002

10.1080/10408399409527668

Sizmur, J. K., and C. W. Walker. 1988. Listeria in prepacked salads. Lanceti:1167.

10.1016/0963-9969(94)90091-4

10.1128/AAC.45.3.649-659.2001

Thomas L. V. M. R. Clarkson and J. Delves-Broughton. 2000. Nisin p. 463-524. In A. S. Naidu (ed.) Natural food antimicrobial systems. CRC Press Boca Raton Fla.

10.4315/0362-028X-65.4.677

10.4315/0362-028X-65.4.709

10.4315/0362-028X-65.6.924

10.1111/j.1574-6976.1993.tb00020.x

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Applied and Environmental Microbiology

Tập 69 Số 8

4519-4526

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