Biocontrol ability of killer yeasts (Saccharomyces cerevisiae) isolated from wine against Colletotrichum gloeosporioides on grape

Journal of Basic Microbiology - Tập 58 Số 1 - Trang 60-67 - 2018
Zongling Liu1, S. H. Du1, Yi Ren1, Yanlin Liu1
1College of Enology Northwest A&F University YangLing Shaanxi China

Tóm tắt

A total of 216 killer yeasts Saccharomyces cerevisiae, isolated from wine, were evaluated in controlling Colletotrichum gloeosporioides, a pre‐harvest anthracnose agent of grape. Three of these yeast isolates were tested positive for antagonizing C. gloeosporioides and were further evaluated for their mechanisms as biological control agents (BCAs): production of antifungal compounds, production of hydrolytic enzymes, inhibition of C. gloeosporioides conidia germination, colonization on grape berry, and efficiency in controlling anthracnose of grape. The results showed that all three S. cerevisiae isolates produced antifungal compounds, inhibited C. gloeosporioides conidia germination and produced β‐1,3‐glucanase and chitinase. All isolates colonized grape berry in large quantities and controlled C. gloeosporioides when artificially inoculated on grape berry. Among the three isolates, application of isolate GA8 resulted in 69.7% of disease reductions for C. gloeosporioides on grape berry. The antagonistic isolates of S. cerevisiae could represent important BCAs of anthracnose of grape caused by C. gloeosporioides that are responsible for economic losses in viticulture.

Từ khóa


Tài liệu tham khảo

Magarey RD, 1993, Anthracnose of grapes, a review, Plant Prot Q, 8, 106

10.1111/j.1365-3059.2007.01698.x

Liu M, 2014, Research progress on grape anthracnose, China Plant Prot, 34, 29

10.1071/APP9930048

10.1016/j.postharvbio.2010.12.010

Gondim DMF, 2013, Use of killer yeast in the management of postharvest papaya anthracnose, Postharvest Bio Tec, 83, 58, 10.1016/j.postharvbio.2013.03.014

Sawant IS, 2016, Biocontrol potential of two novel grapevine associated Bacillus strains for management of anthracnose disease caused by Colletotrichum gloeosporioides, Biocontrol Sci Techn, 26, 1, 10.1080/09583157.2016.1174770

Tan G, 2014, Postharvest biological control of apple anthracnose by Bacillus subtilis BS80‐6, a mutant strain through ion implantation, Plant Dis Pests, 5, 15

10.1111/j.1574-6941.2012.01324.x

10.1016/j.postharvbio.2013.03.014

10.1016/j.micres.2015.04.003

10.1021/pr060483r

10.1094/PHYTO.2002.92.4.393

10.1094/PDIS.2000.84.11.1212

Gulbiniene G, 2004, Occurrence of killer yeast strains in fruit and berry wine yeast populations, Food Technol Biotechnol, 42, 159

10.1371/journal.pone.0165590

10.1016/j.fm.2016.09.005

Grzegorczyk M, 2016, Biocontrol activity of Debaryomyces hansenii, and Wickerhamomyces anomalus, killer yeast strains against phytopatogenic fungi of the species Monilinia, New Biotechnol, 33, S209, 10.1016/j.nbt.2016.06.1441

Ziedan EHE, 2011, Application of yeasts as biocontrol agents for controlling foliar diseases on sugar beet plants, Int J Agr Technol, 7, 1789

Shalaby ES, 2008, Application of Saccharomyces cerevisiae as a biocontrol agent against Fusarium infection of sugar beet plants, Acta Biol Szeged, 59, 271

Ismail AE, 2005, Efficacy of native isolates of Saccharomyces cerevisiae, Trichoderma harzianum and T. ressei in the biocontrol of Meloidogyne incognita and Rotylenchulus reniformis on jasmine in comparison to nematicide vydate under flood irrigation regime in Egypt, Pak J Nematol, 23, 317

Catara A, 2004, Evaluation of Saccharomyces cerevisiae as biocontrol agent of Citrus green mold, Proc Int Soc Citriculture, 2, 725

Attyia SH, 2001, Application of Saccharomyces cerevisiae as a biocontrol agent against some diseases of Solanaceae caused by Macrophomina phaseolina and Fusarium solani, Egypt J Biol, 3, 79

LiL. Study on enology features of non‐Saccharomycesfrom northwest region of China. Dissertation Northwest A&F Uni. 2016.

10.1016/j.biocontrol.2016.03.006

10.1002/jobm.201200049

10.1016/0003-2697(77)90069-0

10.1016/j.foodchem.2003.09.014

10.1007/s12550-014-0189-z

Gong WF, 2016, Control of apple postharvest gray mold by Aureobasidium pullulans, Chin J Biol Control, 32, 251

10.1016/j.carres.2008.02.020

10.1016/j.fm.2014.11.013

10.1016/j.postharvbio.2005.01.001

10.1016/j.ijfoodmicro.2013.09.004