Biochemical basis of insect resistance in inged bean (<i>Psophocarpus tetragonolobus</i>) seeds

Journal of the Science of Food and Agriculture - Tập 55 Số 1 - Trang 63-74 - 1991
Angharad M. R. Gatehouse1, David S Hoe1, Joyce E Flemming1, V. A. Hilder1, John A. Gatehouse1
1Department of Biological Sciences, University of Durham, South Road, Durham, DH1 3LE, UK

Tóm tắt

AbstractMature seeds of the inged bean (Psophocarpus tetragonolobus) are toxic to developing larvae of a range of cosmopolitan storage Bruchidae of economic importance, including the copea seed eevil, Callosobruchus maculatus. Insect feeding trials ere carried out in hich protein fractions from seeds of inged bean ere incorporated at a range of concentrations into artificial seeds, and their effects upon development of C maculatus determined. Both albumin and globulin fractions ere toxic to the developing larvae and their toxicity correlated ith their haemagglutinating activity. Assay of Psophocarpin A, B and C fractions demonstrated Psophocarpin B to be the most insecticidal and to contain the highest haemagglutinating activity. Purified basic seed lectin as highly insecticidal to C maculatus larvae, ith an LC50 value of c. 3·5 g kg−1. The physiological level of this protein in inged bean seeds is sufficient to account for their resistance to attack by C maculatus. inged bean trypsin inhibitor as also purified and tested in artificial seeds against C maculatus. Hoever, even at concentrations in excess of tice the physiological concentration it had no deleterious effects upon development.

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Journal of the Science of Food and Agriculture

Tập 55 Số 1

63-74

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