Bioactive Variability and In Vitro and In Vivo Antioxidant Activity of Unprocessed and Processed Flour of Nine Cultivars of Australian lupin Species: A Comprehensive Substantiation

Antioxidants - Tập 9 Số 4 - Trang 282
Kishor Mazumder1,2, Afia Nabila3, Asma Aktar1, Asgar Farahnaky2,4
1Department of Pharmacy, Jashore University of Science and Technology, Jashore, 7408, Bangladesh
2School of Biomedical Sciences and Graham Centre for Agricultural Innovation, Charles Sturt University, Boorooma St, Wagga Wagga NSW 2127, Australia.
3Department of Pharmacy, Faculty of Basic Medicine and Health Sciences, University of Science and Technology Chittagong, Foy's Lake, Chittagong 4202, Bangladesh.
4School of Science, RMIT University, Bundoora West Campus, Plenty Road, Melbourne, VIC, 3083, Australia

Tóm tắt

The aim of this present investigation was to analyze bioactive compounds, as well as demonstrate the antioxidant activities of nine cultivars of Australian lupin species accompanied by observing the effect of domestic heat processing on their antioxidant activities adopting in vivo and in vitro approaches. Gas chromatography mass spectroscopy (GC-MS) analysis was performed for profiling bioactive compounds present in lupin cultivars. Multiple assay techniques involving quantification of polyphenolics, flavonoids and flavonol, electron transfer (ET) based assay, hydrogen atom transfer (HAT)-based assay and in vivo assays were performed. The major compounds found were hexadecanoic acid methyl ester, 9,12-octadecadienoic acid methyl ester, methyl stearate, lupanine,13-docosenamide and 11-octadecenoic acid (Z)- methyl ester. Mandelup was found to show excellent antioxidant activity. Moreover, Jurien, Gunyidi and Barlock had strong antioxidant activity. Both positive and negative impacts of heat processing were observed on antioxidant activity. Heating and usage of excess water during processing were the key determinants of loss of antioxidants. Negligible loss of antioxidant activity was observed in most of the assays whereas inhibition of both lipid peroxidation (33.53%) and hemolysis of erythrocytes (37.75%) were increased after processing. In addition, in vitro and in vivo antioxidant assays are found to show statistically significant (* p < 0.05 and ** p < 0.01) results, which are supported by the presence of a number of antioxidant compounds in GC-MS analysis.

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Tài liệu tham khảo

Halliwell, 1992, Free radicals, antioxidants, and human disease: Where are we now?, J. Lab. Clin. Med., 119, 598

Young, 2001, Antioxidants in health and disease, J. Clin. Pathol., 54, 176, 10.1136/jcp.54.3.176

Dontha, 2016, A Review on Antioxidant Methods, Asian. J. Pharm. Clin. Res., 9, 14

Scalbert, 2005, Dietary polyphenols and the prevention of diseases, Crit. Rev. Food Sci. Nutr., 45, 287, 10.1080/1040869059096

Robertson, 2005, Metabonomics in Toxicology: A Review, Toxicol. Sci., 85, 809, 10.1093/toxsci/kfi102

Fernie, 2004, Metabolite profiling: From diagnostics to systems biology, Nat. Rev. Mol. Cell Biol., 5, 763, 10.1038/nrm1451

Kell, 2005, Metabolic footprinting and systems biology: The medium is the message, Nat. Rev. Microbiol., 3, 557, 10.1038/nrmicro1177

Rohloff, 2015, Analysis of phenolic and cyclic compounds in plants using derivatization techniques in combination with GC-MS-based metabolite profiling, Molecules, 20, 3431, 10.3390/molecules20023431

Siger, 2012, Antioxidant activity and phenolic content in three lupin species, Subtrop. Plant Sci., 25, 190

Khan, 2015, Phytochemical composition and bioactivities of lupin: A review, Int. J. Food Sci. Tech., 50, 2004, 10.1111/ijfs.12796

French, 2005, Lupin: The largest grain legume crop in Western Australia, its adaptation and improvement through plant breeding, Aust. J. Agric. Res., 56, 1169, 10.1071/AR05088

Kalogeropoulos, 2010, Nutritional evaluation and bioactive microconstituents (phytosterols, tocopherols, polyphenols, triterpenic acids) in cooked dry legumes usually consumed in the Mediterranean countries, Food Chem., 121, 682, 10.1016/j.foodchem.2010.01.005

Turkmen, 2005, The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables, Food Chem., 93, 713, 10.1016/j.foodchem.2004.12.038

Davies, 1990, Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicon and Carbowax 20M phases, J. Chromatogr. A, 503, 1, 10.1016/S0021-9673(01)81487-4

Song, 2010, Total phenolic contents and antioxidant capacities of selected chinese medicinal plants, Int. J. Mol. Sci., 11, 2362, 10.3390/ijms11062362

Fernando, C.D., and Soysa, P. (2014). Total phenolic, flavonoid contents, in-vitro antioxidant activities and hepatoprotective effect of aqueous leaf extract of Atalantia ceylanica. BMC Complementary Altern. Med., 14.

Almaraz, 2007, Antioxidant activity of polyphenolic extract of monofloral honeybee-collected pollen from mesquite (Prosopis juliflora, Leguminosae), J. Food Compos. Anal., 20, 119, 10.1016/j.jfca.2006.08.001

Sahoo, 2013, Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra BL Burtt, Asian Pac. J. Trop. Biomed., 3, 871, 10.1016/S2221-1691(13)60171-9

Ohkawa, 1979, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Anal. Biochem., 95, 351, 10.1016/0003-2697(79)90738-3

Hill, 2012, Small amounts of isotope-reinforced polyunsaturated fatty acids suppress lipid autoxidation, Free Radic. Biol. Med., 53, 893, 10.1016/j.freeradbiomed.2012.06.004

Srinivasan, 2007, Antioxidant activity of Caesalpinia digyna root, J. Ethnopharmacol., 113, 284, 10.1016/j.jep.2007.06.006

Shirwaikar, 2004, In vitro antioxidant studies of Annona squamosa Linn. leaves, Indian J. Exp. Biol., 42, 803

Jayaprakasha, 2004, Antioxidant activities of flavidin in different in vitro model systems, Bioorg. Med. Chem., 12, 5141, 10.1016/j.bmc.2004.07.028

Ebrahimzadeh, 2010, Antihemolytic and antioxidant activities of Allium paradoxum, Cent. Eur. J. Biol., 5, 338

Sinha, 1972, Colorimetric assay of catalase, Anal. Biochem., 47, 389, 10.1016/0003-2697(72)90132-7

McCord, 1969, Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein), J. Biol. Chem., 244, 6049, 10.1016/S0021-9258(18)63504-5

Lowry, 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193, 265, 10.1016/S0021-9258(19)52451-6

Jahan, 2014, Studies on the antioxidant activity of ethanol extract and its fractions from Pterygota alata leaves, J. Acute Med., 4, 103, 10.1016/j.jacme.2014.05.001

Frick, 2018, Characterisation of the genetic factors affecting quinolizidine alkaloid biosynthesis and its response to abiotic stress in narrow-leafed lupin (Lupinus angustifolius L.): Lupin alkaloid biosynthesis and response to stress, Plant Cell Environ., 41, 2155, 10.1111/pce.13172

Giallourou, 2016, Effects of domestic processing methods on the phytochemical content of watercress (Nasturtium officinale), Food Chem., 212, 411, 10.1016/j.foodchem.2016.05.190

Palermo, 2014, The effect of cooking on the phytochemical content of vegetables, J. Sci. Food Agr., 94, 1057, 10.1002/jsfa.6478

Lalitha, 2016, Anticancer potential of pyrrole (1, 2, a) pyrazine 1, 4, dione, hexahydro 3-(2-methyl propyl) (PPDHMP) extracted from a new marine bacterium, Staphylococcus sp. strain MB30, Apoptosis, 21, 566, 10.1007/s10495-016-1221-x

Kalpana, 2012, GC–MS analysis of ethanol extract of Entada pursaetha DC seed, Biosci Disc., 3, 30

Tyagi, 2017, Phytochemical and GC-MS analysis of bioactive constituents in the ethanolic of Pistia stratiotes L. and Eichhornia crassipes (Mart.) solms, J. Pharm. Phytochem., 6, 195

Freire, 2005, Synthesis and antioxidant, anti-inflammatory and gastroprotector activities of anethole and related compounds, Bioorg. Med. Chem., 13, 4353, 10.1016/j.bmc.2005.03.058

Saravana Kumar, P., Al-Dhabi, N.A., Duraipandiyan, V., Balachandran, C., Praveen Kumar, P., and Ignacimuthu, S. (2014). In vitro antimicrobial, antioxidant and cytotoxic properties of Streptomyces lavendulae strain SCA5. BMC Microbiol., 14.

Erdemoglu, 2007, Alkaloid profile and antimicrobial activity of Lupinus angustifolius L. alkaloid extract, Phytochem. Rev., 6, 197, 10.1007/s11101-006-9055-8

Ahmad, 2015, Effect of extraction time on antioxidants and bioactive volatile components of green tea (Camellia sinensis), using GC/MS, Cogent Food Agric., 1, 1106387, 10.1080/23311932.2015.1106387

Caboni, 2012, Nematicidal Activity of (E,E)-2,4-Decadienal and (E)-2-Decenal from Ailanthus altissima against Meloidogyne javanica, J. Agr. Food Chem., 60, 1146, 10.1021/jf2044586

Georgieva, 2018, Morphological and biological characteristics of white lupine cultivars (Lupinus albus L.), Rom. Agric. Res., 35, 109

Mazur, 1966, ”Pharmacologic studies on lupanine and 13-hydroxylupanine”, Acta Phys. Pol., 17, 299

Mazur, 1966, “Pharmacologic studies on 17-oxolupanine, lupanine aminooxide and 17-hydroxylupanine”, Acta Phys. Pol., 17, 311

Wysocka, 2004, Quinolizidine alkaloids isolated from Lupinus species enhance insulin secretion, Eur. J. Pharmacol., 504, 139, 10.1016/j.ejphar.2004.09.008

Wiedemann, 2015, Lupanine Improves Glucose Homeostasis by Influencing KATP Channels and Insulin Gene Expression, Molecules, 20, 19085, 10.3390/molecules201019085

Kolak, 2011, Phytochemical Investigation of Leontice leontopetalum L. subsp. ewersmannii with Antioxidant and Anticholinesterase Activities, Rec. Nat. Prod., 5, 309

Rahman, 2014, Antimicrobial compounds from leaf extracts of Jatropha curcas, Psidium guajava, and Andrographis paniculata, Sci. World J., 2014, 635240, 10.1155/2014/635240

Adnan, M., Nazim Uddin Chy, M., Mostafa Kamal, A.T.M., Azad, M.O.K., Paul, A., Uddin, S.B., Barlow, J.W., Faruque, M.O., Park, C.H., and Cho, D.H. (2019). Investigation of the Biological Activities and Characterization of Bioactive Constituents of Ophiorrhiza rugosa var. prostrata (D.Don) & Mondal Leaves through In Vivo, In Vitro, and In Silico Approaches. Molecules, 24.

Pinto, 2017, Antifungal and antioxidant activity of fatty acid methyl esters from vegetable oils, An. Acad. Bras. Ciênc., 89, 1671, 10.1590/0001-3765201720160908

Andor, 2016, Germinated and Ungerminated Seeds Extract from Two Lupinus Species: Biological Compounds Characterization and In Vitro and In Vivo Evaluations, Evid. Based Complementary Altern. Med., 2016, 8, 10.1155/2016/7638542

Siger, 2003, Chemical composition and antibacterial activities of lupin seeds extracts, Nahrung, 47, 286, 10.1002/food.200390068

Oomah, 2006, Phenolics and antioxidative activities in narrow-leafed lupins (Lupinus angustifolius L.), Plant Food Hum. Nutr., 61, 91, 10.1007/s11130-006-0021-9

Kumar, 2013, Chemistry and biological activities of flavonoids: An overview, Sci. World J., 2013, 162750, 10.1155/2013/162750

Kalam Azad, M.O., Jeong, D.I., Adnan, M., Salitxay, T., Heo, J.W., Naznin, M.T., Lim, J.D., Cho, D.H., Park, B.J., and Park, C.H. (2019). Effect of Different Processing Methods on the Accumulation of the Phenolic Compounds and Antioxidant Profile of Broomcorn Millet (Panicum miliaceum L.) Flour. Foods, 8.

Kruawan, 2012, Antimutagenic and co-mutagenic activities of some legume seeds and their seed coats, J. Med. Plant Res., 6, 3845

Yu, 2013, Comparison of Antioxidant Properties of Refined and Whole Wheat Flour and Bread, Antioxidants, 2, 370, 10.3390/antiox2040370

Chaaban, 2017, Effect of heat processing on thermal stability and antioxidant activity of six flavonoids, J. Food Process. Preserv., 41, e13203, 10.1111/jfpp.13203

Aoyama, 2007, Antioxidant Activity and Flavonoid Content of Welsh Onion (Allium fistulosum) and the Effect of Thermal Treatment, Food Sci. Technol. Res., 13, 67, 10.3136/fstr.13.67

Chaves, 2008, Effect of heat treatment and refrigerated storage on antioxidant properties of pre-cut celery (Apium graveolens L.), Int. J. Food Sci. Tech., 43, 44, 10.1111/j.1365-2621.2006.01380.x

Serpen, 2012, Total antioxidant capacities of raw and cooked meats, Meat Sci., 90, 60, 10.1016/j.meatsci.2011.05.027

Sharma, 2015, Temperature-dependent studies on the total phenolics, flavonoids, antioxidant activities, and sugar content in six onion varieties, J. Food Drug Anal., 23, 243, 10.1016/j.jfda.2014.10.005

Mariscal, 2009, Influence of cooking methods on antioxidant activity of vegetables, J. Food Sci., 74, H97

Nicoli, 1999, Influence of processing on the antioxidant properties of fruit and vegetables, Trends Food Sci. Technol., 10, 94, 10.1016/S0924-2244(99)00023-0

Terpinc, 2011, Antioxidant properties of 4-vinyl derivatives of hydroxycinnamic acid, Food Chem., 128, 62, 10.1016/j.foodchem.2011.02.077

Stepanić, V., Matijašić, M., Horvat, T., Verbanac, D., Kučerová-Chlupáčová, M., Saso, L., and Žarković, N. (2019). Antioxidant Activities of Alkyl Substituted Pyrazine Derivatives of Chalcones-In Vitro and In Silico Study. Antioxidants, 8.

Puntel, 2008, Butane-2,3-dionethiosemicarbazone: An oxime with antioxidant properties, Chem. Biol. Interact., 177, 153, 10.1016/j.cbi.2008.09.028

Tromp, 1996, Structural aspects of antioxidant activity of flavonoids, Free Radic. Biol. Med., 20, 331, 10.1016/0891-5849(95)02047-0

Punithavathi, 2011, Antihyperglycaemic, antilipid peroxidative and antioxidant effects of gallic acid on streptozotocin induced diabetic Wistar rats, Eur. J. Pharmacol., 650, 465, 10.1016/j.ejphar.2010.08.059

Li, 2005, Antioxidant activity of gallic acid from rose flowers in senescence accelerated mice, Life Sci., 77, 230, 10.1016/j.lfs.2004.12.024

Kim, 2007, Antimelanogenic and antioxidant properties of gallic acid, Biol. Pharm. Bull., 30, 1052, 10.1248/bpb.30.1052

Nabavi, 2013, Protective effect of gallic acid isolated from Peltiphyllum peltatum against sodium fluoride-induced oxidative stress in rat’s kidney, Mol. Cell Biochem., 372, 233, 10.1007/s11010-012-1464-y

Kerry, 1997, Red wine and fractionated phenolic compounds prepared from red wine inhibit low density lipoprotein oxidation in vitro, Atherosclerosis, 135, 93, 10.1016/S0021-9150(97)00156-1

Chan, 2001, Flavonoid B-ring chemistry and antioxidant activity: Fast reaction kinetics, Biochem. Biophys. Res. Commun., 282, 1161, 10.1006/bbrc.2001.4705

Gawlik-Dziki, U., Bryda, J., Dziki, D., Swieca, M., Habza-Kowalska, E., and Złotek, U. (2019). Impact of Interactions between Ferulic and Chlorogenic Acids on Enzymatic and Non-Enzymatic Lipids Oxidation: An Example of Bread Enriched with Green Coffee Flour. Appl. Sci., 9.

Shenoy, 2001, Hepatoprotective effects of Ginko biloba against carbon tetrachloride induced hepatic injury in rats, Indian J. Pharmacol., 33, 260

Ahr, 1982, The mechanism of reductive dehalogenation of halothane by liver cytochrome P450, Biochem. Pharmacol., 31, 383, 10.1016/0006-2952(82)90186-1

Maitland, 2006, Hepatoprotective activity of Schouwia thebica Webb, Bioorg. Med. Chem. Lett., 16, 4624, 10.1016/j.bmcl.2006.06.011

Eliza, 2010, Antioxidant activity of costunolide and eremanthin isolated from Costus speciosus (Koen ex. Retz) Sm, Chem. Biol. Interact., 188, 467, 10.1016/j.cbi.2010.08.002

Venkatraman, 1998, Effects of Dietary n-6 and n-3 Lipids on Antioxidant Defense System in Livers of Exercised Rats, J. Am. Coll. Nutr., 17, 586, 10.1080/07315724.1998.10718807

Pompella, 2003, The changing faces of glutathione, a cellular protagonist, Biochem. Pharmacol., 66, 1499, 10.1016/S0006-2952(03)00504-5

Panche, 2016, Flavonoids: An overview, J. Nutr. Sci., 5, 1, 10.1017/jns.2016.41

Wu, 2006, Hepatoprotective effect of total flavonoids from Laggera alata against carbon tetrachloride-induced injury in primary cultured neonatal rat hepatocytes and in rats with hepatic damage, J. Biomed. Sci., 13, 569, 10.1007/s11373-006-9081-y