Phân tích hoạt tính sinh học dưới sự hướng dẫn và nhận diện hóa học bằng HPLC-DAD của saffron Ukraine (tử thi Crocus sativus): từ tương quan đến nguyên nhân

BMC Complementary Medicine and Therapies - Tập 21 - Trang 1-15 - 2021
Olha Mykhailenko1, Vilma Petrikaitė2,3, Michal Korinek4,5,6,7, Mohamed El-Shazly8,9, Bing-Hung Chen5,10, Chia-Hung Yen4, Chung-Fan Hsieh11, Ivan Bezruk1, Asta Dabrišiūtė2, Liudas Ivanauskas12, Victoriya Georgiyants1, Tsong-Long Hwang6,7,13,14
1Department of Pharmaceutical Chemistry, National University of Pharmacy of Ministry of Health of Ukraine, Kharkiv, Ukraine
2Laboratory of Drug Targets Histopathology, Institute of Cardiology, Lithuanian University of Health Sciences, Kaunas, Lithuania
3Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
4Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
5Department of Biotechnology, College of Life Science, Kaohsiung Medical University, Kaohsiung, Taiwan
6Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan, Taiwan
7Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, and Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan
8Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
9Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo, Egypt
10The Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan
11Department of Biochemistry and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan
12Lithuanian University of Health Sciences, Department of Analytical and Toxicological Chemistry, Kaunas, Lithuania
13Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan, Taiwan
14Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City, Taiwan

Tóm tắt

Saffron hay nhuỵ hoa của Crocus sativus L. là một trong những sản phẩm thực phẩm có giá trị cao với các đặc tính thúc đẩy sức khỏe thú vị. C. sativus đã được sử dụng rộng rãi làm chất tạo màu và hương vị. Các hợp chất chuyển hóa thứ cấp trong nhuỵ hoa đã cho thấy hiệu ứng độc tế bào mạnh trong các báo cáo trước đây. Nghiên cứu hiện tại đã điều tra thành phần hóa học và tác động độc tế bào của các chiết xuất thô saffron Ukraine và các hợp chất riêng lẻ đối với các dòng tế bào melanoma IGR39, ung thư vú ba âm MDA-MB-231 và u não U-87 trong ống nghiệm bằng phương pháp thử nghiệm MTT. Nhiều thử nghiệm hoạt tính sinh học in vitro đã được thực hiện. Hồ sơ hóa học của các chiết xuất thô nhuỵ hoa trong nước và hydroethanol (70%, v/v) đã được làm sáng tỏ bằng phân tích HPLC-DAD. Bảy hợp chất đã được xác định bao gồm crocin, picrocrocin, safranal, rutin, apigenin, axit cafeic, axit ferulic. Crocin, picrocrocin, safranal, rutin và apigenin là những thành phần hoạt tính chính của nhuỵ hoa C. sativus Ukraine. Chiết xuất hydroethanol đã giảm đáng kể khả năng sống sót của tế bào MDA-MB-231 và IGR39 và tác động này mạnh hơn so với chiết xuất nước. Tuy nhiên, chiết xuất nước hoạt động gần như gấp 5,6 lần so với dòng tế bào U-87 (EC50 của chiết xuất nước đối với U-87 là 0,15 ± 0,02 mg/mL, và EC50 của chiết xuất hydroethanol là 0,83 ± 0,03 mg/mL). Các hợp chất tinh khiết như apigenin và axit cafeic cũng cho thấy hoạt tính độc tế bào cao đối với các dòng tế bào ung thư vú, melanoma và u não. Sàng lọc các hoạt tính sinh học của chiết xuất nước từ nhuỵ hoa (lên đến 100 μg/mL) bao gồm tác dụng kháng dị ứng, kháng virus, ức chế neuraminidase và chống viêm đã tiết lộ hoạt tính ức chế của nó đối với enzyme neuraminidase đạt 41%. Các kết quả được trình bày đã làm rõ thành phần hóa học định tính và định lượng cũng như hoạt tính sinh học của nhuỵ hoa Crocus sativus từ Ukraine như một nguồn cung cấp các tác nhân chống ung thư tự nhiên và ức chế neuraminidase. Kết quả hoạt tính sinh học của các chiết xuất cho thấy tiềm năng ứng dụng trong tương lai của saffron như một phương thuốc tự nhiên chống lại nhiều loại ung thư.

Từ khóa

#saffron #Crocus sativus #hoạt tính sinh học #độc tế bào #HPLC-DAD #ung thư #neuraminidase

Tài liệu tham khảo

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