Bid-dependent generation of oxygen radicals promotes death receptor activation-induced apoptosis in murine hepatocytes

Hepatology - Tập 40 Số 2 - Trang 403-413 - 2004
Wen-Xing Ding1, Clíona Ní Cheallaigh1, Daniell DiFrancesca1, Donna B. Stolz2, Xiao-Ming Yin1,3
1Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA
2Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA
3fax: (412) 648-9564

Tóm tắt

Activation of tumor necrosis factor receptor 1 or Fas leads to the generation of reactive oxygen species, which are important to the cytotoxic effects of tumor necrosis factor α (TNF-α) or Fas ligand. However, how these radicals are generated following receptor ligation is not clear. Using primary hepatocytes, we found that TNF-α or anti-Fas antibody-induced burst of oxygen radicals was mainly derived from the mitochondria. We discovered that Bid—a pro-death Bcl-2 family protein activated by ligated death receptors—was the main intracellular molecule signaling the generation of the radicals by targeting to the mitochondria and that the majority of oxygen radical production was dependent on Bid. Reactive oxygen species contributed to cell death and caspase activation by promoting FLICE-inhibitory protein degradation and mitochondrial release of cytochrome c. For the latter part, the oxygen radicals did not affect Bak oligomerization but instead promoted mitochondrial cristae reorganization and membrane lipid peroxidation. Antioxidants could reverse these changes and therefore protect against TNF-α or anti-Fas-induced apoptosis. In conclusion, our studies established the signaling pathway from death receptor engagement to oxygen radical generation and determined the mechanism by which reactive oxygen species contributed to hepatocyte apoptosis following death receptor activation. (Hepatology 2004;40:403-413.)

Từ khóa


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Hepatology

Tập 40 Số 2

403-413

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