Bacterial Bioluminescence: Isolation and Expression of the Luciferase Genes from Vibrio harveyi

American Association for the Advancement of Science (AAAS) - Tập 218 Số 4574 - Trang 791-793 - 1982
Robert Belas1, Alan J. Mileham1, Daniel H. Cohn1, Marcia Hilman1, Melvin I. Simon1, Melvin Silverman1
1Agouron Institute, La Jolla 92037, California, USA.

Tóm tắt

Genes for the luciferase enzyme of Vibrio harveyi were isolated in Escherichia coli by a general method in which nonluminous, transposon insertion mutants were used. Conditions necessary for light production in E. coli were examined. Stimulation of transcription of the genes for luciferase ( lux A and lux B ) was required for efficient synthesis of luciferase. To enhance transcription bacteriophage promoter elements were coupled to the cloned lux gene fragments.

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Tài liệu tham khảo

BELAS R unpublished data.

CASADABAN, M.J., INVITRO GENE FUSIONS THAT JOIN AN ENZYMATICALLY ACTIVE BETA-GALACTOSIDASE SEGMENT TO AMINO-TERMINAL FRAGMENTS OF EXOGENOUS PROTEINS - ESCHERICHIA-COLI PLASMID VECTORS FOR THE DETECTION AND CLONING OF TRANSLATIONAL INITIATION SIGNALS, JOURNAL OF BACTERIOLOGY 143: 971 (1980).

Cohn, D. H., Proceedings of the National Academy of Sciences of the United States of America 80: 120 (1983).

HASTINGS, J.W., TOTAL QUANTUM FLUX OF ISOTROPIC SOURCES, JOURNAL OF THE OPTICAL SOCIETY OF AMERICA 53: 1410 (1963).

HEDGPETH, J, LAMBDA-PHAGE PROMOTER USED TO ENHANCE EXPRESSION OF A PLASMID-CLONED GENE, MOLECULAR & GENERAL GENETICS 163: 197 (1978).

KARL, D. M., JOURNAL OF GENERAL MICROBIOLOGY 117: 357 (1980).

KEYNAN, A, BIOL BULL 121: 375 (1961).

MILLER, J.H., EXPT MOL GENETICS: 433 (1977).

MURRAY, N.E., LAMBDOID PHAGES THAT SIMPLIFY RECOVERY OF INVITRO RECOMBINANTS, MOLECULAR & GENERAL GENETICS 150: 53 (1977).

NEALSON, K.H., ARCH MICROBIOL 112: 83 (1977).

NEALSON, K.H., METHODS IN ENZYMOLOGY 57: 153 (1978).

NEALSON, K.H., BACTERIAL BIOLUMINESCENCE - ITS CONTROL AND ECOLOGICAL SIGNIFICANCE, MICROBIOLOGICAL REVIEWS 43: 496 (1979).

NICOLI M.Z. THESIS HARVARD U (1972).

RIENDEAU, D, EVIDENCE FOR A FATTY-ACID REDUCTASE CATALYZING THE SYNTHESIS OF ALDEHYDES FOR THE BACTERIAL BIOLUMINESCENT REACTION, JOURNAL OF BIOLOGICAL CHEMISTRY 254: 7488 (1979).

ROSSON, R.A., AUTOINDUCTION OF BACTERIAL BIOLUMINESCENCE IN A CARBON LIMITED CHEMOSTAT, ARCHIVES OF MICROBIOLOGY 129: 299 (1981).

SILVERMAN, M, PHASE VARIATION IN SALMONELLA - GENETIC-ANALYSIS OF A RECOMBINATIONAL SWITCH, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 391 (1979).

10.1016/S0022-2836(75)80083-0

STRUBER, D, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 78: 167 (1981).

ZIEGLER, M.M., BIOCHEMISTRY OF BACTERIAL BIOLUMINESCENCE, CURRENT TOPICS IN BIOENERGETICS 12: 65 (1981).

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American Association for the Advancement of Science (AAAS)

Tập 218 Số 4574

791-793

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