BRAD, the genetics and genomics database for Brassica plants

Feng Cheng1, Liu S2, Jian Wu1, Fang Lü1, Silong Sun1, Bo Liu1, Pingxia Li1, Wei Hua2, Xiaowu Wang1
1Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2The Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China

Tóm tắt

Abstract Background

Brassica species include both vegetable and oilseed crops, which are very important to the daily life of common human beings. Meanwhile, the Brassica species represent an excellent system for studying numerous aspects of plant biology, specifically for the analysis of genome evolution following polyploidy, so it is also very important for scientific research. Now, the genome of Brassica rapa has already been assembled, it is the time to do deep mining of the genome data.

Description

BRAD, the Brassica database, is a web-based resource focusing on genome scale genetic and genomic data for important Brassica crops. BRAD was built based on the first whole genome sequence and on further data analysis of the Brassica A genome species, Brassica rapa (Chiifu-401-42). It provides datasets, such as the complete genome sequence of B. rapa, which was de novo assembled from Illumina GA II short reads and from BAC clone sequences, predicted genes and associated annotations, non coding RNAs, transposable elements (TE), B. rapa genes' orthologous to those in A. thaliana, as well as genetic markers and linkage maps. BRAD offers useful searching and data mining tools, including search across annotation datasets, search for syntenic or non-syntenic orthologs, and to search the flanking regions of a certain target, as well as the tools of BLAST and Gbrowse. BRAD allows users to enter almost any kind of information, such as a B. rapa or A. thaliana gene ID, physical position or genetic marker.

Conclusion

BRAD, a new database which focuses on the genetics and genomics of the Brassica plants has been developed, it aims at helping scientists and breeders to fully and efficiently use the information of genome data of Brassica plants. BRAD will be continuously updated and can be accessed through http://brassicadb.org.

Từ khóa


Tài liệu tham khảo

Labana KS, Gupta ML: Importance and origin. in Breeding Oilseed Brassicas (eds. Labana, K.S., Banga, S.S. & Banga, S.K.). Springer-Verlag, Berlin. 1993, 1-20.

Beilstein MA, Al-Shehbaz IA, EA K: Brassicaceae phylogeny and trichome evolution. Am J Bot. 2006, 93: 607-619. 10.3732/ajb.93.4.607.

UN: Genome analysis in Brassica with special reference to theexperimental formation of B. napus and peculiar mode of fertilication.Jap J Bot 1935, , 7: 389-452.

Yang TJ, Kim JS, Kwon SJ, Lim KB, Choi BS, Kim JA, Jin M, Park JY, Lim MH, Kim HI, et al: Sequence-level analysis of the diploidization process in the triplicated FLOWERING LOCUS C region of Brassica rapa. Plant Cell. 2006, 18 (6): 1339-1347. 10.1105/tpc.105.040535.

Mun JH, Kwon SJ, Yang TJ, Seol YJ, Jin M, Kim JA, Lim MH, Kim JS, Baek S, Choi BS, et al: Genome-wide comparative analysis of the Brassica rapa gene space reveals genome shrinkage and differential loss of duplicated genes after whole genome triplication. Genome Biol. 2009, 10 (10): R111-10.1186/gb-2009-10-10-r111.

Wang X, Wang H, Wang J, Sun R, Wu J, Liu S, Bai Y, Mun JH, Bancroft I, Cheng F, et al: The genome of the mesopolyploid crop species Brassica rapa. Nat Genet. 2011.

Kim H, Choi SR, Bae J, Hong CP, Lee SY, Hossain MJ, Van Nguyen D, Jin M, Park BS, Bang JW, et al: Sequenced BAC anchored reference genetic map that reconciles the ten individual chromosomes of Brassica rapa. BMC Genomics. 2009, 10: 432-10.1186/1471-2164-10-432.

Choi SR, Teakle GR, Plaha P, Kim JH, Allender CJ, Beynon E, Piao ZY, Soengas P, Han TH, King GJ, et al: The reference genetic linkage map for the multinational Brassica rapa genome sequencing project. Theor Appl Genet. 2007, 115 (6): 777-792. 10.1007/s00122-007-0608-z.

Wang Y, Sun S, Liu B, Wang H, Deng J, Liao Y, Wang Q, Cheng F, Wang X, Wu J: A sequence-based genetic linkage map as a reference for Brassica rapa pseudochromosome assembly. BMC Genomics. 2011, 12: 239-10.1186/1471-2164-12-239.

Donlin MJ: Using the Generic Genome Browser (GBrowse). Curr Protoc Bioinformatics. 2009, Chapter 9: Unit 9 9.