BLCA prognostic model creation and validation based on immune gene-metabolic gene combination

Hormones and Cancer - Tập 14 - Trang 1-21 - 2023
Shao-Yu Yue1,2,3, Di Niu1,2,3, Xian-Hong Liu1,2,3, Wei-Yi Li1,2,3, Ke Ding1,2,3, Hong-Ye Fang1,2,3, Xin-Dong Wu1,2,3, Chun Li1,2,3, Yu Guan1,2,3, He-Xi Du1,2,3
1Department of Urology, the First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, People’s Republic of China
2Institute of Urology, Anhui Medical University, Hefei, People’s Republic of China
3Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, People’s Republic of China

Tóm tắt

Bladder cancer (BLCA) is a prevalent urinary system malignancy. Understanding the interplay of immunological and metabolic genes in BLCA is crucial for prognosis and treatment. Immune/metabolism genes were extracted, their expression profiles analyzed. NMF clustering found prognostic genes. Immunocyte infiltration and tumor microenvironment were examined. Risk prognostic signature using Cox/LASSO methods was developed. Immunological Microenvironment and functional enrichment analysis explored. Immunotherapy response and somatic mutations evaluated. RT-qPCR validated gene expression. We investigated these genes in 614 BLCA samples, identifying relevant prognostic genes. We developed a predictive feature and signature comprising 7 genes (POLE2, AHNAK, SHMT2, NR2F1, TFRC, OAS1, CHKB). This immune and metabolism-related gene (IMRG) signature showed superior predictive performance across multiple datasets and was independent of clinical indicators. Immunotherapy response and immune cell infiltration correlated with the risk score. Functional enrichment analysis revealed distinct biological pathways between low- and high-risk groups. The signature demonstrated higher prediction accuracy than other signatures. qRT-PCR confirmed differential gene expression and immunotherapy response. The model in our work is a novel assessment tool to measure immunotherapy’s effectiveness and anticipate BLCA patients’ prognosis, offering new avenues for immunological biomarkers and targeted treatments.

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