BAF250B-Associated SWI/SNF Chromatin-Remodeling Complex Is Required to Maintain Undifferentiated Mouse Embryonic Stem Cells

Stem Cells - Tập 26 Số 5 - Trang 1155-1165 - 2008
Zhijiang Yan1, Zhong Wang2, Lioudmila V. Sharova3, Alexei A. Sharov3, Ling Chen1, Yulan Piao3, Keisuke Aiba3, Ryo Matoba3, Weidong Wang1, Minoru S.H. Ko3
1Genome Instability and Chromatin-Remodeling Section, National Institutes of Health, Baltimore, Maryland, USA
2Cardiovascular Research Center, Massachusetts General Hospital, Richard B. Simches Research Center, Harvard Medical School, Boston, Massachusetts, USA
3Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA

Tóm tắt

Abstract Whether SWI/SNF chromatin remodeling complexes play roles in embryonic stem (ES) cells remains unknown. Here we show that SWI/SNF complexes are present in mouse ES cells, and their composition is dynamically regulated upon induction of ES cell differentiation. For example, the SWI/SNF purified from undifferentiated ES cells contains a high level of BAF155 and a low level of BAF170 (both of which are homologs of yeast SWI3 protein), whereas that from differentiated cells contains nearly equal amounts of both. Moreover, the levels of BAF250A and BAF250B decrease during the differentiation of ES cells, whereas that of BRM increases. The altered expression of SWI/SNF components hinted that these complexes could play roles in ES cell maintenance or differentiation. We therefore generated ES cells with biallelic inactivation of BAF250B and found that these cells display a reduced proliferation rate and an abnormal cell cycle. Importantly, these cells are deficient in the self-renewal capacity of undifferentiated ES cells and exhibit certain phenotypes of differentiated cells, including reduced expression of several pluripotency-related genes and increased expression of some differentiation-related genes. These data suggest that the BAF250B-associated SWI/SNF is essential for mouse ES cells to maintain their normal proliferation and pluripotency. The work presented here underscores the importance of SWI/SNF chromatin remodeling complexes in pluripotent stem cells. Disclosure of potential conflicts of interest is found at the end of this article.

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Stem Cells

Tập 26 Số 5

1155-1165

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