Walton H. Marsh1, Benjamin P. Fingerhut1, Henry Miller1
1Department of Pathology, State University of New York, Downstate Medical Center, and the Institute of Pathology, Kings County Hospital Center, Brooklyn, N. Y. 11203
Tóm tắt
AbstractAutomated and manual direct methods for the determination of urea in blood or serum are described. These methods determine urea by the colored product formed when urea, in relatively weak acid solution, reacts with diacetyl monoxime in the presence of thiosemicarbazide and ferric ion. Results are compared with those obtained by urease conversion of urea to ammonia and measurement of the ammonia by nesslerization.