Xiaomei Yan1, Lihuan Cao1, Qiang Li1, Yanhua Wu1, Haoxing Zhang1, Hexige Saiyin1, Xiang-hua Liu1, Xuqing Zhang1, Qinghua Shi2, Long Yu1
1State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China.
2Department of Cell Biology, Harvard Medical School, Boston, MA 02115 USA
Tóm tắt
Much recent attention has been focused on Aurora C, the third member of the mammalian Aurora kinases family that plays significant roles in mitosis. We report here that using sensitive RT‐PCR to amplify the C‐terminal, we found that Aurora C is not only expressed highly in testis, but also among 16 other human tissues in a broad‐spectrum way. Aurora C, as a chromosomal passenger protein, is co‐localized with Aurora B and Survivin in mitotic cells. Aurora C can also be associated with Aurora B and Survivin in vivo and directly binds to Survivin but not Aurora B in vitro. Over‐expression of a catalytically inactive mutant of Aurora C impaired the localization of Aurora C to the spindle midzone and severely disturbed the cytokinesis, resulting in multinucleation, all of which are consistent with the results induced by the mutant of Aurora B. Furthermore, we provide evidence that Aurora C could rescue the multinucleate phenotype produced by Aurora B mutant, and vice versa. Overall, these findings demonstrate that Aurora C, a member of the chromosomal passenger complex, is required for cytokinesis.
