Aurora-A kinase is required for centrosome maturation in <i>Caenorhabditis elegans </i>

Journal of Cell Biology - Tập 155 Số 7 - Trang 1109-1116 - 2001
Eva Hannak1,2, Matthew Kirkham1, Anthony A. Hyman1, Karen Oegema1,2
11Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
22European Molecular Biology Laboratory, 69117 Heidelberg, Germany

Tóm tắt

Centrosomes mature as cells enter mitosis, accumulating γ-tubulin and other pericentriolar material (PCM) components. This occurs concomitant with an increase in the number of centrosomally organized microtubules (MTs). Here, we use RNA-mediated interference (RNAi) to examine the role of the aurora-A kinase, AIR-1, during centrosome maturation in Caenorhabditis elegans. In air-1(RNAi) embryos, centrosomes separate normally, an event that occurs before maturation in C. elegans. After nuclear envelope breakdown, the separated centrosomes collapse together, and spindle assembly fails. In mitotic air-1(RNAi) embryos, centrosomal α-tubulin fluorescence intensity accumulates to only 40% of wild-type levels, suggesting a defect in the maturation process. Consistent with this hypothesis, we find that AIR-1 is required for the increase in centrosomal γ-tubulin and two other PCM components, ZYG-9 and CeGrip, as embryos enter mitosis. Furthermore, the AIR-1–dependent increase in centrosomal γ-tubulin does not require MTs. These results suggest that aurora-A kinases are required to execute a MT-independent pathway for the recruitment of PCM during centrosome maturation.

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Journal of Cell Biology

Tập 155 Số 7

1109-1116

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