AtHMA3, a P1B-ATPase Allowing Cd/Zn/Co/Pb Vacuolar Storage in Arabidopsis

Oxford University Press (OUP) - Tập 149 Số 2 - Trang 894-904 - 2009
Mélanie Morel‐Rouhier1, Jérôme Crouzet1, Antoine Gravot1, Fred Beisson1, Nathalie Leonhardt1, Alain Vavasseur1, Pierre Richaud1
1Commissariat à l'Energie Atomique, DSV, IBEB, Lab Echanges Membran and Signalisation, Saint-Paul-lez-Durance F–13108, France; CNRS, UMR Biol Veget and Microbiol Environ, Saint-Paul-lez-Durance F–13108, France; and Aix-Marseille Université, Saint-Paul-lez-Durance F–13108, France

Tóm tắt

Abstract The Arabidopsis (Arabidopsis thaliana) Heavy Metal Associated3 (AtHMA3) protein belongs to the P1B-2 subgroup of the P-type ATPase family, which is involved in heavy metal transport. In a previous study, we have shown, using heterologous expression in the yeast Saccharomyces cerevisiae, that in the presence of toxic metals, AtHMA3 was able to phenotypically complement the cadmium/lead (Cd/Pb)-hypersensitive strain ycf1 but not the zinc (Zn)-hypersensitive strain zrc1. In this study, we demonstrate that AtHMA3 in planta is located in the vacuolar membrane, with a high expression level in guard cells, hydathodes, vascular tissues, and the root apex. Confocal imaging in the presence of the Zn/Cd fluorescent probe BTC-5N revealed that AtHMA3 participates in the vacuolar storage of Cd. A T-DNA insertional mutant was found more sensitive to Zn and Cd. Conversely, ectopic overexpression of AtHMA3 improved plant tolerance to Cd, cobalt, Pb, and Zn; Cd accumulation increased by about 2- to 3-fold in plants overexpressing AtHMA3 compared with wild-type plants. Thus, AtHMA3 likely plays a role in the detoxification of biological (Zn) and nonbiological (Cd, cobalt, and Pb) heavy metals by participating in their vacuolar sequestration, an original function for a P1B-2 ATPase in a multicellular eukaryote.

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Oxford University Press (OUP)

Tập 149 Số 2

894-904

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