Mối liên hệ giữa sự tiếp xúc với Asen và các biến đổi toàn cầu của histone sau phiên mã ở người lớn tại Bangladesh
Tóm tắt
Nền tảng: Sự tiếp xúc với asen (As) có liên quan đến nguy cơ gia tăng của nhiều loại ung thư cũng như các bệnh tim mạch, và các sự thiếu hụt phát triển thần kinh ở trẻ em. Các hợp chất asen có tính đột biến yếu, làm thay đổi biểu hiện gen và các biến đổi histone sau phiên mã (PTHMs) in vitro.
Phương pháp: Nồng độ As trong nước và nước tiểu cũng như các mức độ toàn cầu của sự đột biến di-methylation và acetylation lysine 9 histone 3 (H3K9me2 và H3K9ac), di-methylation và acetylation lysine 27 histone 3 (H3K27me3 và H3K27ac), acetylation lysine 18 histone 3 (H3K18ac) và trimethylation lysine 4 histone 3 (H3K4me3) đã được đo ở các tế bào đơn nhân máu ngoại vi (PBMC) từ một tập hợp nhỏ của những người tham gia (N = 40) của một thí nghiệm lâm sàng về folate tại Bangladesh (nghiên cứu FACT).
Kết quả: Tổng nồng độ As trong nước tiểu (uAs) có mối tương quan tích cực với H3K9me2 (r = 0,36, P = 0,02) và liên quan nghịch với H3K9ac (r = −0,47, P = 0,002). Các mối liên kết giữa As và các PTHMs khác có sự khác biệt theo giới tính. As trong nước (wAs) có mối tương quan tích cực với H3K4me3 (r = 0,45, P = 0,05) và H3K27me3 (r = 0,50, P = 0,03) ở nữ và có mối tương quan nghịch ở nam (H3K4me3: r = −0,44, P = 0,05; H3K27me3: r = −0,34, P = 0,14). Ngược lại, wAs có mối liên hệ nghịch với H3K27ac ở nữ (r = −0,44, P = 0,05) và mối liên kết tích cực ở nam (r = 0,29, P = 0,21). Một mô hình tương tự đã được quan sát đối với H3K18ac (nữ: r = −0,22, P = 0,36; nam: r = 0,27, P = 0,24).
Kết luận: Sự tiếp xúc với As có liên quan đến các biến đổi toàn cầu của PTHMs; các mẫu liên kết riêng theo giới tính đã được quan sát giữa sự tiếp xúc với As và một số dấu hiệu histone.
Ảnh hưởng: Những phát hiện này góp phần vào cơ sở bằng chứng ngày càng gia tăng liên kết sự tiếp xúc với As với sự rối loạn biểu sinh, có thể đóng vai trò trong sinh bệnh học của độc tính As. Epidemiol Ung thư Biomarkers Prev; 21(12); 2252–60. ©2012 AACR.
Từ khóa
Tài liệu tham khảo
Kinniburgh, 2001, Arsenic contamination of groundwater in Bangladesh
Bank, 2005, Towards a more effective operational response
Kumar, 2010, Arsenic exposure in US public and domestic drinking water supplies: a comparative risk assessment, J Expo Sci Environ Epidemiol, 245
IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, 2004, Some drinking-water disinfectants and contaminants, including Arsenic
Schuhmacher-Wolz, 2009, Oral exposure to inorganic arsenic: evaluation of its carcinogenic and non-carcinogenic effects, Crit Rev Toxicol, 39, 271, 10.1080/10408440802291505
Chiu, 2004, Does arsenic exposure increase the risk for liver cancer?, J Toxicol Environ Health A, 67, 1491, 10.1080/15287390490486806
Benbrahim-Tallaa, 2008, Inorganic arsenic and human prostate cancer, Environ Health Perspect, 116, 158, 10.1289/ehp.10423
Rahman, 2009, Chronic exposure of arsenic via drinking water and its adverse health impacts on humans, Environ Geochem Health, 31, 189, 10.1007/s10653-008-9235-0
Wasserman, 2007, Water arsenic exposure and intellectual function in 6-year-old children in Araihazar, Bangladesh, Environ Health Perspect, 115, 285, 10.1289/ehp.9501
Chen, 2009, Arsenic exposure at low-to-moderate levels and skin lesions, arsenic metabolism, neurological functions, and biomarkers for respiratory and cardiovascular diseases: review of recent findings from the Health Effects of Arsenic Longitudinal Study (HEALS) in Bangladesh, Toxicol Appl Pharmacol, 239, 184, 10.1016/j.taap.2009.01.010
Tseng, 2003, Long-term arsenic exposure and ischemic heart disease in arseniasis-hyperendemic villages in Taiwan, Toxicol Lett, 137, 15, 10.1016/S0378-4274(02)00377-6
Wasserman, 2004, Water arsenic exposure and children's intellectual function in Araihazar, Bangladesh, Environ Health Perspect, 112, 1329, 10.1289/ehp.6964
Chen, 1995, Increased prevalence of hypertension and long-term arsenic exposure, Hypertension, 25, 53, 10.1161/01.HYP.25.1.53
Pilsner, 2007, Genomic methylation of peripheral blood leukocyte DNA: influences of arsenic and folate in Bangladeshi adults, Am J Clin Nutr, 86, 1179, 10.1093/ajcn/86.4.1179
Ren, 2011, An emerging role for epigenetic dysregulation in arsenic toxicity and carcinogenesis, Environ Health Perspect, 119, 11, 10.1289/ehp.1002114
Berger, 2007, The complex language of chromatin regulation during transcription, Nature, 447, 407, 10.1038/nature05915
Esteller, 2007, Cancer epigenomics: DNA methylomes and histone-modification maps, Nat Rev Genet, 8, 286, 10.1038/nrg2005
Schneider, 2007, Dynamics and interplay of nuclear architecture, genome organization, and gene expression, Genes Dev, 21, 3027, 10.1101/gad.1604607
Creyghton, 2010, Histone H3K27ac separates active from poised enhancers and predicts developmental state, Proc Natl Acad Sci U S A, 107, 21931, 10.1073/pnas.1016071107
Lorenzen, 2012, Epigenetic modifications in cardiovascular disease, Basic Res Cardiol, 107, 245, 10.1007/s00395-012-0245-9
Kurdistani, 2011, Histone modifications in cancer biology and prognosis, Prog Drug Res, 67, 91
Jakovcevski, 2012, Epigenetic mechanisms in neurological disease, Nat Med, 18, 1194, 10.1038/nm.2828
Arita, 2012, Global levels of histone modifications in peripheral blood mononuclear cells of subjects with exposure to nickel, Environ Health Perspect, 120, 198, 10.1289/ehp.1104140
Cantone, 2011, Inhalable metal-rich air particles and histone H3K4 dimethylation and H3K9 acetylation in a cross-sectional study of steel workers, Environ Health Perspect, 119, 964, 10.1289/ehp.1002955
Zhou, 2009, Effects of nickel, chromate, and arsenite on histone 3 lysine methylation, Toxicol Appl Pharmacol, 236, 78, 10.1016/j.taap.2009.01.009
Ramirez, 2008, Sodium arsenite modulates histone acetylation, histone deacetylase activity and HMGN protein dynamics in human cells, Chromosoma, 117, 147, 10.1007/s00412-007-0133-5
Jensen, 2009, Epigenetic mediated transcriptional activation of WNT5A participates in arsenical-associated malignant transformation, Toxicol Appl Pharmacol, 235, 39, 10.1016/j.taap.2008.10.013
Arrigo, 1983, Acetylation and methylation patterns of core histones are modified after heat or arsenite treatment of Drosophila tissue culture cells, Nucleic Acids Res, 11, 1389, 10.1093/nar/11.5.1389
Jensen, 2008, Epigenetic remodeling during arsenical-induced malignant transformation, Carcinogenesis, 29, 1500, 10.1093/carcin/bgn102
Jo, 2009, Acetylated H4K16 by MYST1 protects UROtsa cells from arsenic toxicity and is decreased following chronic arsenic exposure, Toxicol Appl Pharmacol, 241, 294, 10.1016/j.taap.2009.08.027
Zhou, 2008, Arsenite alters global histone H3 methylation, Carcinogenesis, 29, 1831, 10.1093/carcin/bgn063
Li, 2002, Arsenic trioxide promotes histone H3 phosphoacetylation at the chromatin of CASPASE-10 in acute promyelocytic leukemia cells, J Biol Chem, 277, 49504, 10.1074/jbc.M207836200
Li, 2003, Tumor promoter arsenite stimulates histone H3 phosphoacetylation of proto-oncogenes c-fos and c-jun chromatin in human diploid fibroblasts, J Biol Chem, 278, 13183, 10.1074/jbc.M300269200
Pilsner, 2007, Genomic methylation of peripheral blood leukocyte DNA: influences of arsenic and folate in Bangladeshi adults, Am J Clin Nutr, 86, 1179, 10.1093/ajcn/86.4.1179
Pilsner, 2009, Folate deficiency, hyperhomocysteinemia, low urinary creatinine, and hypomethylation of leukocyte DNA are risk factors for arsenic-induced skin lesions, Environ Health Perspect, 117, 254, 10.1289/ehp.11872
Geoffroy, 2010, Arsenic-induced SUMO-dependent recruitment of RNF4 into PML nuclear bodies, Mol Biol Cell, 21, 4227, 10.1091/mbc.e10-05-0449
Zhu, 2002, How acute promyelocytic leukaemia revived arsenic, Nat Rev Cancer, 2, 705, 10.1038/nrc887
Platanias, 2009, Biological responses to arsenic compounds, J Biol Chem, 284, 18583, 10.1074/jbc.R900003200
Slot, 1965, The significance of systemic arteriovenous difference in creatinine clearence determinations, Scand J Clin Lab Invest, 17, 201, 10.3109/00365516509075336
Chen, 2006, Nickel ions increase histone H3 lysine 9 dimethylation and induce transgene silencing, Mol Cell Biol, 26, 3728, 10.1128/MCB.26.10.3728-3737.2006
Latham, 2007, Cross-regulation of histone modifications, Nat Struct Mol Biol, 14, 1017, 10.1038/nsmb1307
Cedar, 2009, Linking DNA methylation and histone modification: patterns and paradigms, Nat Rev Genet, 10, 295, 10.1038/nrg2540
Kile, 2012, Prenatal arsenic exposure and DNA methylation in maternal and umbilical cord blood leukocytes, Environ Health Perspect, 120, 1061, 10.1289/ehp.1104173
Jackson, 2004, Dimethylation of histone H3 lysine 9 is a critical mark for DNA methylation and gene silencing in Arabidopsis thaliana, Chromosoma, 112, 308, 10.1007/s00412-004-0275-7
Chanda, 2006, DNA hypermethylation of promoter of gene p53 and p16 in arsenic-exposed people with and without malignancy, Toxicol Sci, 89, 431, 10.1093/toxsci/kfj030
Mass, 1997, Arsenic alters cytosine methylation patterns of the promoter of the tumor suppressor gene p53 in human lung cells: a model for a mechanism of carcinogenesis, Mutat Res, 386, 263, 10.1016/S1383-5742(97)00008-2
Minardi, 2009, Prognostic role of global DNA-methylation and histone acetylation in pT1a clear cell renal carcinoma in partial nephrectomy specimens, J Cell Mol Med, 13, 2115, 10.1111/j.1582-4934.2008.00482.x
Agrawal-Singh, 2012, Genome-wide analysis of histone H3 acetylation patterns in AML identifies PRDX2 as an epigenetically silenced tumor suppressor gene, Blood, 119, 2346, 10.1182/blood-2011-06-358705
Bredfeldt, 2010, Xenoestrogen-induced regulation of EZH2 and histone methylation via estrogen receptor signaling to PI3K/AKT, Mol Endocrinol, 24, 993, 10.1210/me.2009-0438
Davey, 2007, Arsenic as an endocrine disruptor: effects of arsenic on estrogen receptor-mediated gene expression in vivo and in cell culture, Toxicol Sci, 98, 75, 10.1093/toxsci/kfm013
Lee, 2005, Mechanisms for the inhibition of DNA methyltransferases by tea catechins and bioflavonoids, Mol Pharmacol, 68, 1018, 10.1124/mol.104.008367
De Kimpe, 1999, In vitro methylation of arsenite by rabbit liver cytosol: effect of metal ions, metal chelating agents, methyltransferase inhibitors and uremic toxins, Drug Chem Toxicol, 22, 613, 10.3109/01480549908993171
Cheng, 2010, Coordinated chromatin control: structural and functional linkage of DNA and histone methylation, Biochemistry, 49, 2999, 10.1021/bi100213t
Voet D, 1990, Biochemistry
2003, Arsenic exposure and health effects