Assessment of the Pharmacological Properties and Phytochemical Profile of Bruguiera gymnorhiza (L.) Lam Using In Vitro Studies, In Silico Docking, and Multivariate Analysis

Biomolecules - Tập 10 Số 5 - Trang 731
Nabeelah Bibi Sadeer1, Kouadio Ibrahime Sinan2, Zoltán Cziáky3, József Jekö3, Gökhan Zengin2, Rajesh Jeewon1, Hassan H. Abdallah4, Kannan R. R. Rengasamy5, Mohamad Fawzi Mahomoodally6,7
1Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, Mauritius
2Department of Biology, Science Faculty, Selcuk University, 42130, Konya, Turkey
3Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, 4400 Nyíregyháza, Hungary
4Chemistry Department, College of education, Salahaddin University-Erbil, 44001 Erbil, Iraq
5Department of Bioresources and Food Science, College of Life Sciences, Konkuk University, Seoul 05029, Korea
6Department for Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh 758307, Vietnam
7Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh 758307, Vietnam

Tóm tắt

Bruguiera gymnorhiza (L.) Lam. is claimed to effectively manage a number of ailments including diabetes and associated complications. Nonetheless, no attempt has been made to delineate its pharmacological propensities and phytochemical profile. This study was designed to appraise the antioxidant and enzymatic inhibitory properties relevant to the management of diabetes mellitus, obesity, and neurodegenerative and skin disorders. A combination of colorimetric assays and ultra-high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) were applied for the phytochemical screening of leaf, root, twig, and fruit extracts (methanol and ethyl acetate). In vitro antioxidant evaluations were via radical scavenging abilities (DPPH, ABTS), reducing potential (FRAP, CUPRAC), chelating power, and total antioxidant capacity (phosphomolybdenum). Seven key metabolic enzymes (α-amylase, α-glucosidase, tyrosinase, elastase, lipase, AChE, and BChE) were targeted to determine the inhibitory effects. Multivariate and in silico docking analysis were performed on collected data. Methanolic fruit extract yielded the highest total phenolic, tannin, and triterpenoid contents (174.18 ± 4.27 mg GAE/g, 176.24 ± 3.10 mg CE/g, 63.11 ± 3.27 mg OAE/g, respectively); significantly depressed tyrosinase, elastase, and α-amylase activities (155.35 ± 0.29 mg KAE/g, 4.56 ± 0.10 mg CAE/g, 1.00 ± 0.05 mmol ACAE/g, accordingly); and harboured the most potent antioxidant capacities with DPPH, CUPRAC, FRAP (492.62 ± 5.31, 961.46 ± 11.18, 552.49 ± 8.71 mg TE/g, respectively), and phosphomolybdenum (4.17 ± 0.31 mmol TE/g) assays. Multivariate analysis suggested that the type of solvents used influenced the biological activities more compared to plant parts. Docking analysis showed that azelaic acid binds with tyrosinase by Van der Waals and conventional hydrogen bonds. We anticipate that the present study may establish baseline data on this halophyte that could open new avenues for the development of biomedicine.

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Biomolecules

Tập 10 Số 5

731

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