Assay for Hydralazine as Its Stable p-Nitrobenzaldehyde Hydrazone

Pharmaceutical Research - Tập 5 Số 6 - Trang 383-386 - 1988
Semple, Hugh A.1, Tam, Yun K.1, Tin, Sarah1, Courts, Ronald T.1
1Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Canada

Tóm tắt

A new method of analysis for the antihypertensive drug, hydralazine, is introduced. The assay involves the addition of p-nitrobenzaldehyde to blood samples containing hydralazine, to form a stable Schiff's base, hydralazine p-nitrobenzaldehyde hydrazone. The derivative is extracted from the blood into hexane and the samples are dried under a nitrogen stream. The extracts are then dissolved in mobile phase and analyzed using high-performance liquid chromatography. The extracted samples can be stored for at least 7 days at room temperature or at −20°C. The sensitivity of the assay is better than 300 pg/ml using 3-ml blood samples, and the range can extend to 640 ng/ml. The stability of the extracted samples plus the sensitivity and simplicity of the assay are the main advantages of the method over other selective methods for hydralazine.

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Pharmaceutical Research

Tập 5 Số 6

383-386

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