Assay for Antibody in Pig Fetuses Infected with Porcine Parvovirus

Acta Veterinaria Scandinavica - Tập 21 - Trang 312-317 - 1980
K. J. Sørensen1, J. Askaa1, K. Dalsgaard1
1State Veterinary Institute for Virus Research, Lindholm, Denmark

Tóm tắt

Fetal fluids from field cases of fetal death were assayed for antibody to porcine parvovirus (PPV) using 3 different techniques. An indirect immunofluorescent antibody test, a counter immunoelectrophoresis test and a hemagglutination inhibition test were compared. The indirect immunofluorescent antibody test was found to be the most sensitive of the tests employed. The hemagglutination inhibition test apparently suffered from the occurrence of false positive results.

Tài liệu tham khảo

Bachmann, P. A.: Vorkommen und Verbreitung von Picodna (Parvo)-Virus beim Schwein. (Occurrence and distribution of Picodna (Parvo) viruses (PPV) in the pig). Zbl. Vet.-Med. 1969, 16 B, 341–345. Cartwright, S. F., M. Lucas & R. A. Huck: A small haemagglutinating porcine DNA virus. I. Isolation and properties. J. comp. Path. 1969, 79, 371–377. Croxton, F. E.: Elementary Statistics with Application in Medicine and the Bioloigical Sciences. Dover Publications, Inc. New York 1959, 209–244. Dalsgaard, K., E. Overby, J. J. Metzger & A. Basse: Rapid method for screening of immunoglobulins in porcine fetuses, using rocket immunoelectrophoresis. Acta vet. scand. 1979, 20, 313–320. Hallauer, C., G. Kronauer & G. Siegl: Parvoviruses as contaminants of permanent human cell line. I. Virus isolations from 1960–1970. Arch. ges. Virusforsch. 1971, 35, 80–90. Harboe, N. & A. Ingild: Immunization, isolation of immunoglobulins, estimation of antibody titre. In Axelsen, N. H., J. Krøll & B. Weeke, (eds.): A Manual of Quantitative Immunoelectrophoresis, Methods and Applications. 161–164. Universitetsforlaget, Oslo 1973. Johnson, R. H.: Isolation of swine parvovirus in Queensland. Aust. vet. J. 1973, 49, 157–159. Johnson, R. H. & D. F. Collings: Transplacental infection of piglets with a porcine parvovirus. Res. vet. Sci. 1971, 12, 570–572. Joo, H. S., C. R. Donaldson-Wood & R. H. Johnson: A microneutralization test for the assay of porcine parvovirus antibody. Arch. Virol. 1975, 47, 337–341. Joo, H. S., C. R. Donaldson-Wood & R. H. Johnson: Rapid diagnostic techniques for detection of porcine parvovirus infection in mummified foetuses. Aust. vet. J. 1976, 52, 51–52. Mengeling, W. L.: Porcine parvovirus: Properties and prevalence of a strain isolated in the United States. Amer. J. vet. Res. 1972, 33, 2239–2248. Mengeling, W. L.: Prevalence of porcine parvovirusinduced reproductive failure: An abattoir study. J. Amer. vet. med. Ass. 1978, 172, 1291–1294. The, T. H. & T. E. W. Feltkamp: Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method. Immunology 1970, 18, 875–881.