Ascorbate peroxidase – a hydrogen peroxide‐scavenging enzyme in plants

Physiologia Plantarum - Tập 85 Số 2 - Trang 235-241 - 1992
Kozi Asada1
1The Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan

Tóm tắt

Ascorbate peroxidase is a hydrogen peroxide‐scavenging enzyme that is specific to plants and algae and is indispensable to protect chloroplasts and other cell constituents from damage by hydrogen peroxide and hydroxyl radicals produced from it. In this review, first, the participation of ascorbate peroxidase in the scavenging of hydrogen peroxide in chloroplasts is briefly described. Subsequently, the phylogenic distribution of ascorbate peroxidase in relation to other hydrogen peroxide‐scavenging peroxidases using glutathione, NADH and cytochrome c is summarized. Chloroplastic and cytosolic isozymes of ascorbate peroxidase have been found, and show some differences in enzymatic properties. The basic properties of ascorbate peroxidases, however, are very different from those of the guaiacol peroxidases so far isolated from plant tissues. Amino acid sequence and other molecular properties indicate that ascorbate peroxidase resembles cytochrome c peroxidase from fungi rather than guaiacol peroxidase from plants, and it is proposed that the plant and yeast hydrogen peroxide‐scavenging peroxidases have the same ancestor.

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Tài liệu tham khảo

10.1016/0167-4838(89)90086-1

10.1016/0005-2728(83)90026-9

Asada K., 1984, Photoreduction of 18O2 and H2 18O2 with a concomitant evolution of 18O2 in intact spinach chloroplasts; Evidence for scavenging of hydrogen peroxide by peroxidase, Plant Cell Physiol., 25, 1169

Asada K., 1987, Topics in Photosynthesis, 227

10.1016/S0021-9258(19)42815-9

Bagchi S. N., 1991, The effect of activated oxygen species on nitrogenase of Anabaena variabilis, Z. Naturforsch., 46, 407, 10.1515/znc-1991-5-612

10.1042/bj1880643

10.1093/oxfordjournals.pcp.a077713

Chen G.‐X., 1990, Hydroxyurea and p‐aminophenol are the suicide inhibitors of ascorbate peroxidase, J. Biol. Chem., 265, 2775, 10.1016/S0021-9258(19)39869-2

Chen G.‐X., 1992, Inactivation of ascorbate peroxidase by thiols requires hydrogen peroxide, Plant Cell Physiol., 33, 117

Chen G.‐X., 1992, Amino acid sequence of ascorbate peroxidase has a homology with that of cytochrome c peroxidase from yeast, Plant Cell Physiol., 33, 109

10.1104/pp.83.4.789

10.1016/0168-9452(85)90025-1

10.1002/9780470123102.ch1

10.1007/BF00386001

10.1016/0005-2728(83)90047-6

Gander J. E., 1982, Encyclopedia of Plant Physiology, New Series, 77

10.1016/S0176-1617(84)80051-6

10.1016/0005-2728(79)90078-1

Hossain M. A., 1984, Purification of dehydroascorbate reductase from spinach and its characterization as a thiol enzyme, Plant Cell Physiol., 25, 85

Hossain M. A., 1984, Inactivation of ascorbate peroxidase in spinach chloroplasts on dark addition of hydrogen peroxide: Its protection by ascorbate, Plant Cell Physiol., 25, 1285

Hossain M. A., 1985, Monodehydroascorbate reductase from cucumber is a flavin adenine dinucleotide enzyme, J. Biol. Chem., 260, 12920, 10.1016/S0021-9258(17)38813-0

Hossain M. A., 1984, Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide, Plant Cell Physiol., 25, 385

10.1016/S0031-9422(00)84931-6

10.1016/0005-2728(76)90035-9

Kaput J., 1982, Nucleotide sequence of the yeast nuclear gene for cytochrome c peroxidase precursor, J. Biol. Chem., 257, 15054, 10.1016/S0021-9258(18)33392-1

10.3109/02713688809033196

Klapheck S., 1990, Scavenging of hydrogen peroxide in the endosperm of Ricinus communis by ascorbate peroxidase, Plant Cell Physiol., 31, 1005

10.1007/BF00020011

10.1007/BF00428286

10.1016/0003-9861(51)90082-3

10.1007/BF00248605

10.1104/pp.97.3.962

10.1016/0014-5793(91)81083-K

10.3109/10715769109088937

Miyake C., 1991, Scavenging of hydrogen peroxide in prokaryotic and eukaryotic algae: Acquisition of ascorbate peroxidase during the evolution of cyanobacteria, Plant Cell Physiol., 32, 33

Nakano Y., 1981, Hydrogen peroxide is scavenged by ascorbate‐specific peroxidase in spinach chloroplasts, Plant Cell Physiol., 22, 867

Nakano Y., 1987, Purification of ascorbate peroxidase in spinach chloroplasts: Its inactivation in ascorbate‐depleted medium and reactivation by monodehydroascorbate radical, Plant Cell Physiol., 28, 131

10.1104/pp.85.1.294

10.1104/pp.77.2.437

10.1016/0968-0004(90)90152-2

10.1104/pp.86.2.626

10.1016/S0031-9422(00)88177-7

10.1271/bbb1961.43.2053

10.1016/0003-9861(80)90495-6

10.1016/0305-0491(79)90231-1

10.1016/0003-9861(88)90505-X

Svirbely J. L., 1933, XL. The chemical nature of vitamin C, Biochem. J., 27, 279

10.1093/oxfordjournals.pcp.a076315

10.1016/0003-9861(88)90080-X

10.1016/0003-9861(91)90053-L

Tartaglia L. A., 1990, Alkyl hydroperoxide reductase from Salmonella typhimurium, J. Biol. Chem., 265, 10535, 10.1016/S0021-9258(18)86980-0

10.1016/0003-9861(86)90485-6

10.1111/j.1432-1033.1985.tb09129.x

10.1104/pp.86.3.649