Artemisia capillaris inhibited enterovirus 71‐induced cell injury by preventing viral internalization

The Kaohsiung Journal of Medical Sciences - Tập 34 - Trang 150-159 - 2018
Ming-Hong Yen1, Ching-I. Huang2,3, Min-Sheng Lee4, Ya-Ping Cheng5, Chia Jung Hsieh6, Lien-Chai Chiang7, Jung-San Chang5,8
1School of Pharmacy and Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Taiwan
2Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
3Hepatobiliary Division, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
4Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Taiwan
5Department of Renal Care, College of Medicine, Kaohsiung Medical University, Taiwan
6Department of Traditional Chinese Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
7Department of Microbiology, School of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan
8Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Taiwan

Tóm tắt

AbstractArtemisia capillaris (A. capillaris) is a common herbal drug used for thousands years in ancient China. A. capillaris has been empirically used to manage hand‐foot‐mouth disease (HFMD), which is commonly caused by enterovirus 71 (EV71). EV71 can cause meningoencephalitis with mortality and neurologic sequelae without effective management. It is presently unknown whether A. capillaris is effective against EV71 infection. To test the hypothesis that it could protect cells from EV71‐induced injury, a hot water extract of A. capillaris was tested in human foreskin fibroblast cells (CCFS‐1/KMC) and human rhabdomyosarcoma cells (RD cells) by plaque reduction assay and flow cytometry. Inhibition of viral replication was examined by reverse quantitative RT‐PCR (qRT‐PCR). Its effect on translations of viral proteins (VP0, VP1, VP2, protease 2B and 3AB), and apoptotic proteins were examined by western blot. A. capillaris was dose‐dependently effective against EV71 infection in both CCFS‐1/KMC cells and RD cells by inhibiting viral internalization. However, A. capillaris was minimally effective on viral attachment, VP2 translation, and inhibition of virus‐induced apoptosis. Further isolation of effective molecules is needed. In conclusion, A. capillaris has anti‐EV71 activity mainly by inhibiting viral internalization. A. capillaris would be better to manage EV71 infection in combination with other agents.

Tài liệu tham khảo

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Thông tin xuất bản

The Kaohsiung Journal of Medical Sciences

Tập 34

150-159

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