Chiết xuất tỏi trong nước cải thiện sự thanh thải thận thông qua cơ chế giãn mạch/kháng oxy hóa và giảm protein niệu thông qua việc ổn định hàng rào lọc cầu thận

Springer Science and Business Media LLC - Tập 5 Số 1 - Trang 1-13 - 2019
Imafidon, Christian Eseigbe1,2, Akomolafe, Rufus Ojo2, Eluwole, Omotayo Alaba3, Adekunle, Isiaka Ayofe2, Agbaje, Ruby Adebusola4
1Renal Research Laboratory, Department of Physiology, Faculty of Basic Medical and Health Sciences, Bowen University Iwo, Iwo, Nigeria
2Department of Physiological Sciences, Faculty of Basic Medical Sciences, Obafemi Awolowo University, Ile-Ife, Nigeria
3Department of Medical Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, Obafemi Awolowo University, Ile-Ife, Nigeria
4Department of Biochemistry and Molecular Biology, Faculty of Sciences, Obafemi Awolowo University, Ile-Ife, Nigeria

Tóm tắt

Chì (Pb) vẫn là một vật liệu dường như không thể thiếu trong một số quy trình công nghiệp. Nó là một chất độc hại cho môi trường với các tác động sinh học có hại liên quan. Nghiên cứu đã điều tra tác động của chiết xuất tỏi trong nước (AGE) lên sự thanh thải thận và protein niệu ở chuột Wistar bị tổn thương thận do Pb. Ba mươi con chuột Wistar đực được chia thành sáu nhóm, mỗi nhóm gồm năm con, trong đó việc tiếp xúc với Pb (35 mg/kg qua đường tiêm phúc mạc) trong 10 ngày liên tục được theo sau bởi 30 ngày hồi phục (không điều trị) hoặc 30 ngày sau điều trị với liều lượng AGE đường miệng từ 100, 200 và 400 mg/kg, đồng thời so sánh với một nhóm đối chứng (2 ml/kg dung dịch muối sinh lý). Độc tính của Pb đã gây ra những thay đổi có hại trong các chỉ số chức năng thận (creatinine, ure và protein tổng) trong huyết tương và nước tiểu, các chỉ thị của stress oxy hóa và quá trình peroxit hóa lipid (GSH, SOD, CAT và TBARS) trong mô thận cùng với sự giảm đáng kể nồng độ NO trong huyết tương và thận (p < 0.05) của chuột. Nó cũng làm giảm đáng kể thanh thải creatinine và tỷ lệ bài tiết ure, trong khi tỷ lệ protein tổng trong nước tiểu/creatinine tăng đáng kể ở chuột. Giải phẫu thận cho thấy có bằng chứng về giảm kích thước tiểu cầu thận do Pb với sự thoái hóa ống và mô kẽ. Tuy nhiên, việc sử dụng AGE liên quan đến sự phục hồi đáng kể các thông số sinh hóa đã đề cập (p < 0.05) cũng như sự cải thiện cấu trúc mô thận. Các tác động dược lý của AGE được quy cho các thành phần hóa học thực vật xác định của nó. AGE đã làm bình thường hóa sự thanh thải thận thông qua các cơ chế giãn mạch và chống oxy hóa, đồng thời giảm protein niệu thông qua việc ổn định hàng rào lọc cầu thận.

Từ khóa

#Chì #chiết xuất tỏi trong nước #thanh thải thận #protein niệu #chuột Wistar

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