Antiviral activity of silver nanoparticle/chitosan composites against H1N1 influenza A virus
Tóm tắt
Silver nanoparticle (Ag NP)/chitosan (Ch) composites with antiviral activity against H1N1 influenza A virus were prepared. The Ag NP/Ch composites were obtained as yellow or brown floc-like powders following reaction at room temperature in aqueous medium. Ag NPs (3.5, 6.5, and 12.9 nm average diameters) were embedded into the chitosan matrix without aggregation or size alternation. The antiviral activity of the Ag NP/Ch composites was evaluated by comparing the TCID50ratio of viral suspensions treated with the composites to untreated suspensions. For all sizes of Ag NPs tested, antiviral activity against H1N1 influenza A virus increased as the concentration of Ag NPs increased; chitosan alone exhibited no antiviral activity. Size dependence of the Ag NPs on antiviral activity was also observed: antiviral activity was generally stronger with smaller Ag NPs in the composites. These results indicate that Ag NP/Ch composites interacting with viruses exhibit antiviral activity.
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Pal S, Tak YK, Song JM: Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli. Appl Environ Microbiol 2007, 73: 1712–1720. 10.1128/AEM.02218-06
Sondi I, Salopek-Sondi B: Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci 2004, 275: 177–182. 10.1016/j.jcis.2004.02.012
Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, Yacaman MJ: The bactericidal effect of silver nanoparticles. Nanotechnology 2005, 16: 2346–2353. 10.1088/0957-4484/16/10/059
Gajbhiye M, Kesharwani J, Ingle A, Gade A, Rai M: Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomedicine 2009, 5: 382–386. 10.1016/j.nano.2009.06.005
Liau SY, Read DC, Pugh WJ, Furr JR, Russell AD: Interaction of silver nitrate with readily identifiable groups: relationship to the antibacterial action of silver ions. Lett Appl Microbiol 1997, 25: 279–283. 10.1046/j.1472-765X.1997.00219.x
Elechiguerra J, Burt JL, Morones JR, Camacho-Bragado A, Gao X, Lara HH, Yacaman M: Interaction of silver nanoparticles with HIV-1. J Nanobiotechnology 2005, 3: 6. 10.1186/1477-3155-3-6
Trefry JC, Wooley DP: Rapid assessment of antiviral activity and cytotoxicity of silver nanoparticles using a novel application of the tetrazolium-based colorimetric assay. J Virol Methods 2012, 183: 19–24. 10.1016/j.jviromet.2012.03.014
Lu L, Sun RW, Chen R, Hui CK, Ho CM, Luk JM, Lau GK, Che CM: Silver nanoparticles inhibit hepatitis B virus replication. Antivir Ther 2008, 13: 253–262.
Baram-Pinto D, Shukla S, Perkas N, Gedanken A, Sarid R: Inhibition of herpes simplex virus type 1 infection by silver nanoparticles capped with mercaptoethane sulfonate. Bioconjugate Chem 2009, 20: 1497–1502. 10.1021/bc900215b
Sun L, Singh AK, Vig K, Pillai SR, Singh SR: Silver nanoparticles inhibit replication of respiratory syncytial virus. J Biomed Nanotechnol 2008, 4: 149–158.
Rogers JV, Parkinson CV, Choi YW, Speshock JL, Hussain SM: A preliminary assessment of silver nanoparticle inhibition of monkeypox virus plaque formation. Nanoscale Res Lett 2008, 3: 129–133. 10.1007/s11671-008-9128-2
Speshock JL, Murdock RC, Braydich-Stolle LK, Schrand AM, Hussain SM: Interaction of silver nanoparticles with Tacaribe virus. J Nanobiotechnology 2010, 8: 19. 10.1186/1477-3155-8-19
Mehrbod P, Motamed N, Tabatabaian M, Soleimani ER, Amini E, Shahidi M, Kheiri MT: In vitro antiviral effect of "nanosilver" on influenza virus. DARU J Pharm Sci 2009, 17: 88–93.
Xiang DX, Chen Q, Pang L, Zheng CL: Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro. J Virol Methods 2011, 178: 137–142. 10.1016/j.jviromet.2011.09.003
Wise JP Sr, Goodale BC, Wise SS, Craig GA, Pongan AF, Walter RB, Thompson WD, Ng AK, Aboueissa AM, Mitani H, Spalding MJ, Mason MD: Silver nanospheres are cytotoxic and genotoxic to fish cells. Aquat Toxicol 2010, 97: 34–41. 10.1016/j.aquatox.2009.11.016
Navarro E, Piccapietra F, Wagner B, Marconi F, Kaegi R, Odzak N, Sigg L, Behra R: Toxicity of silver nanoparticles to Chlamydomonas reinhardtii. Environ Sci Technol 2008, 42: 8959–8964. 10.1021/es801785m
Braydich-Stolle LK, Lucas B, Schrand A, Murdock RC, Lee T, Schlager JJ, Hussain SM, Hofmann MC: Silver nanoparticles disrupt GDNF/Fyn kinase signaling in spermatogonial stem cells. Toxicol Sci 2010, 116: 577–589. 10.1093/toxsci/kfq148
Matyjas-Zgondek E, Bacciarelli A, Rybicki E, Szynkowska MI, Kołodziejczyk M: Antibacterial properties of silver-finished textiles. Fibres Text East Eur 2008, 16: 101–107.
Filipowska B, Rybicki E, Walawska A, Matyjas-Zgondek E: New method for the antibacterial and antifungal modification of silver finished textiles. Fibres Text East Eur 2011, 19: 124–128.
Murugadoss A, Chattopadhyay A: A ‘green’ chitosan–silver nanoparticle composite as a heterogeneous as well as micro-heterogeneous catalyst. Nanotechnology 2008, 19: 015603. 10.1088/0957-4484/19/01/015603
Damm C, Münstedt H: Kinetic aspects of the silver ion release from antimicrobial polyamide/silver nanocomposites. Appl Phys A 2008, 91: 479–486. 10.1007/s00339-008-4434-1
Sanpui P, Murugadoss A, Prasad PV, Ghosh SS, Chattopadhyay A: The antibacterial properties of a novel chitosan-Ag-nanoparticle composite. Int J Food Microbiol 2008, 124: 142–146. 10.1016/j.ijfoodmicro.2008.03.004
Fayaz AM, Ao Z, Girilal M, Chen L, Xiao X, Kalaichelvan PT, Yao X: Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection. Int J Nanomed 2012, 7: 5007–5018.
Shi C, Zhu Y, Ran X, Wang M, Su Y, Cheng T: Therapeutic potential of chitosan and its derivatives in regenerative medicine. J Surg Res 2006, 133: 185–192. 10.1016/j.jss.2005.12.013
Mori Y, Tagawa T, Fujita M, Kuno T, Suzuki S, Matsui T, Ishihara M: Simple and environmentally friendly preparation and size control of silver nanoparticles using an inhomogeneous system with silver-containing glass powder. J Nanopart Res 2011, 13: 2799–2806. 10.1007/s11051-010-0168-z
Reed LJ, Muench H: A simple method of estimating fifty per cent endpoints. Am J Hyg 1938, 27: 493–497.
LaBarre DD, Lowy RJ: Improvements in methods for calculating virus titer estimates from TCID50 and plaque assays. J Virol Methods 2001, 96: 107–126. 10.1016/S0166-0934(01)00316-0
An J, Luo Q, Yuan X, Wang D, Li X: Preparation and characterization of silver-chitosan nanocomposite particles with antimicrobial activity. J Appl Polym Sci 2011, 120: 3180–3189. 10.1002/app.33532
Sosa IO, Noguez C, Barrera RG: Optical properties of metal nanoparticles with arbitrary shapes. J Phys Chem B 2003, 107: 6269–6275. 10.1021/jp0274076