Antiviral Activity of the Proteasome on Incoming Human Immunodeficiency Virus Type 1

Journal of Virology - Tập 72 Số 5 - Trang 3845-3850 - 1998
Olivier Schwartz1, Valérie Maréchal1, Bertrand Friguet2, Fernando Arenzana‐Seisdedos3, Jean‐Michel Heard1
1Laboratoire Rétrovirus et Transfert Génétique, URA CNRS 1157,1
2Unité de Biochimie Cellulaire,2 and
3Unitéd’Immunologie Virale,3 Institut Pasteur, 75724 Paris Cedex 15, France

Tóm tắt

ABSTRACT Following cell surface receptor binding and membrane fusion, human immunodeficiency virus (HIV) virion cores are released in the cytoplasm. Incoming viral proteins represent potential targets for cytosolic proteases. We show that treatment of target cells with the proteasome inhibitors MG132 and lactacystin increased the efficiency of HIV infection. Proteasome inhibitors were active at the early steps of the viral cycle. Incoming p24 Gag proteins accumulated in the cytosol, and larger amounts of proviral DNA were synthesized. In vitro, purified 20S proteasome degraded HIV virion components. Thus, degradation of incoming viral proteins by the proteasome represents an early intracellular defense against infection.

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Journal of Virology

Tập 72 Số 5

3845-3850

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