Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts

Foods - Tập 10 Số 2 - Trang 309
Romina Lis Ferrero1, Carmen Soto‐Maldonado2,1, Caroline Weinstein‐Oppenheimer3,4, Zaida Cabrera1, María Elvira Zúñiga‐Hansen2,1
1Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, Chile
2Centro Regional de Estudio en Alimentos Saludables, R17A10001, Av. Universidad 330, Curauma, Valparaíso 2373223, Chile
3Centro de Investigación Farmacopea Chilena, Santa Marta 183, Playa Ancha, Valparaíso 2360134, Chile
4Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Gran Bretaña 1093, Playa Ancha, Valparaíso 2360102, Chile

Tóm tắt

Defatted rapeseed meal (DRM) is a sub-valorized agro-industrial by-product, with a high protein content whose peptides could have potential anticancer activity against cancer cell lines. The objective of the present study is to obtain an enzymatic hydrolysate of rapeseed protein that inhibits proliferation on a breast cancer cell line (MCF-7), but not healthy human fibroblast cells. The DRM was solubilized in an alkaline medium to obtain an alkaline rapeseed extract (RAE). Acid precipitation of the proteins contained in RAE recovered a rapeseed protein isolate (RPI). To produce protein hydrolysates, two alkaline protease and different enzyme/substrate ratios were used. All the protein hydrolysates showed antiproliferative activity on MCF-7 cells. However, only the hydrolysate recovered from the enzymatic hydrolysis of RPI (Degree of hydrolysis (DH ) between 8.5 and 9% (DH1)) did not affect human fibroblast cells, inhibiting 83.9% of MCF-7 cells’ proliferation and showing a mass yield of 22.9% (based on the initial DRM). The SDS-PAGE gel revealed that DH1 was composed mainly of 10 kDa peptides and, to a lesser extent, 5 and 2 kDa. It is concluded that DH1 is a promising peptide extract for future research as a putative anti-breast cancer agent.

Từ khóa


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