Antioxidant and free radical scavenging activity of Spondias pinnata

BMC Complementary and Alternative Medicine - Tập 8 Số 1 - 2008
Bibhabasu Hazra1, Santanu Biswas1, Nripendranath Mandal1
1Division of Molecular Medicine, Bose Institute, P-1/12 CIT Scheme VIIM, Kolkata, 700054, India

Tóm tắt

AbstractBackgroundMany diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally-occurring antioxidants of plant origin. The aim of the present study was to evaluate thein vitroantioxidant activities ofSpondias pinnatastem bark extract.MethodsA 70% methanol extract ofSpondias pinnatastem bark was studiedin vitrofor total antioxidant activity, for scavenging of hydroxyl radicals, superoxide anions, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen and hypochlorous acid, and for iron chelating capacity, reducing power, and phenolic and flavonoid contents.ResultsThe extract showed total antioxidant activity with a trolox equivalent antioxidant concentration (TEAC) value of 0.78 ± 0.02. The IC50values for scavenging of free radicals were 112.18 ± 3.27 μg/ml, 13.46 ± 0.66 μg/ml and 24.48 ± 2.31 μg/ml for hydroxyl, superoxide and nitric oxide, respectively. The IC50for hydrogen peroxide scavenging was 44.74 ± 25.61 mg/ml. For the peroxynitrite, singlet oxygen and hypochlorous acid scavenging activities the IC50values were 716.32 ± 32.25 μg/ml, 58.07 ± 5.36 μg/ml and 127.99 ± 6.26 μg/ml, respectively. The extract was found to be a potent iron chelator with IC50= 66.54 ± 0.84 μg/ml. The reducing power was increased with increasing amounts of extract. The plant extract (100 mg) yielded 91.47 ± 0.004 mg/ml gallic acid-equivalent phenolic content and 350.5 ± 0.004 mg/ml quercetin-equivalent flavonoid content.ConclusionThe present study provides evidence that a 70% methanol extract ofSpondias pinnatastem bark is a potential source of natural antioxidants.

Từ khóa


Tài liệu tham khảo

Braca A, Sortino C, Politi M, Morelli I, Mendez J: Antioxidant activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol. 2002, 79: 379-381.

Maxwell SR: Prospects for the use of antioxidant therapies. Drugs. 1995, 49: 345-361.

Niki E, Shimaski H, Mino M: Antioxidantism-free radical and biological defense. Gakkai Syuppn Center, Tokyo. 1994, 3-16.

Tandon S, Rastogi RP: Studies on the chemical constituents of Spondias pinnata. Planta Med. 1976, 29: 190-192.

Ghosal PK, Thakur S: Structural features of the acidic polysaccharide of Spondias pinnata gum exudates. Carbohydr Res. 1981, 98: 75-83.

Bibitha B, Jisha VK, Salitha CV, Mohan S, Valsa AK: Antibacterial activity of different plant extracts. Indian J Microbiol. 2002, 42 (4): 361-363.

Mahanta RK, Rout SD, Sahu HK: Ethnomedicinal plant resources of Similipal biosphere reserve, Orissa, India. Zoos Print J. 2006, 21 (8): 2372-2374.

Leksomboon C, Thaveechai N, Kositratana W: Potential of plant extracts for controlling citrus canker of lime. Kasetsart J (Nat Sci). 2001, 35: 392-396.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C: Antioxidant activity applying an improved ABTS radical cation decolorization asszy. Free Rad Biol Med. 1999, 26: 1231-1237.

Elizabeth K, Rao MNA: Oxygen radical scavenging activity of curcumin. Int J Pharmaceut. 1990, 58: 237-240.

Fontana M, Mosca L, Rosei MA: Interaction of enkephalines with oxyradicals. Biochem Pharmacol. 2001, 61: 1253-1257.

Garratt DC: The quantitative analysis of Drugs. 1964, Chapman and Hall ltd, Japan, 3: 456-458.

Long LH, Evans PJ, Halliwell B: Hydrogen peroxide in human urine: implications for antioxidant defense and redox regulation. Biochem Biophys Res Commun. 1999, 262: 605-609.

Floriano-Sánchez E, Villanueva C, Medina-Campos ON, Rocha D, Sánchez-González DJ, Cárdenas-Rodríguez N, Pedraza-Chaverrí J: Nordihydroguaiaretic acid is a potent in vitro scavenger of peroxynitrite, singlet oxygen, hydroxyl radical, superoxide anion, andhypochlorous acid and prevents in vivo tyrosine nitration in lung. Free Radic Res. 2006, 40: 523-533.

Beckman JS, Chen H, Ischiropulos H, Crow JP: Oxidative chemistry of peroxynitrite. Methods Enzymol. 1994, 233: 229-240.

Bailly F, Zoete V, Vamecq J, Catteu JP, Bernier JL: Antioxidant actions of ovothiol-derived 4-mercaptoimidazoles: glutathione peroxidase activity and protection against peroxynitrite-induced damage. FEBS Lett. 2000, 486: 19-22.

Chakraborty N, Tripathy BC: Involvement of singlet oxygen in 5-aminolevulinic acid-induced photodynamic damage of cucumber (Cucumbis sativus L.) chloroplasts. Plant Physiol. 1992, 98: 7-11.

Pedraza-Chaverrí J, Barrera D, Maldonado PD, Chirino YI, Macías-Ruvalcaba NA, Medina-Campos ON, Castro L, Salcedo MI, Hernández-Pando R: S-allylmercaptocysteine scavenges hydroxyl radical and singlet oxygen in vitro and attenuates gentamicininduced oxidative and nitrosative stress and renal damage in vivo. BMC Clin Pharmacol. 2004, 4: 5-

Aruoma OI, Halliwell B: Action of hypochlorous acid on the antioxidant protective enzymes superoxide dismutase, catalase and glutathione peroxidase. Biochem J. 1987, 248: 973-976.

Pedraza-Chaverrí J, Arriaga-Noblecía G, Medina-Campos ON: Hypochlorous acid scavenging capacity of garlic. Phytother Res. 2007, 21: 884-888.

Haro-Vicente JF, Martinez-Gracia C, Ros G: Optimization of in vitro measurement of available iron from different fortificants in citric fruit juices. Food Chem. 2006, 98: 639-648.

Oyaizu M: Studies on products of browning reactions: antioxidant activities of products of browning reaction prepared from glucose amine. Jap J Nutr. 1986, 44: 307-315.

Singleton VL, Rossi JA: Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic. 1965, 16: 144-158.

Zhishen J, Mengcheng T, Jianming W: The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999, 64: 555-559.

Halliwell B, Gutteridge JM: Free radicals in biology and medicine. 1998, Oxford: Oxford University Press

Yazdanparast R, Ardestani A: In vitro antioxidant and free radical scavenging activity of Cyperus rotundus. J Med Food. 2007, 10: 667-674.

Yazdanparast R, Bahramikias S, Ardestani A: Nasturtium oficinale reduces oxidative stress and enhances antioxidant capacity in hypercholesterolaemic rats. Chem Biol Interact. 2008, 172: 176-184.

Packer L, Ong ASH: Biological oxidants and antioxidants: Molecular mechanisms and health effects. 1997, Champaign, IL: AOCS Press

Jovanovic SV, Simic MG: Antioxidants in nutrition. Ann NY Acad Sci. 2000, 899: 326-334.

Mai TT, Thu NN, Tien PG, Chuyen NV: Alpha-Glucosidase inhibitory and antioxidant activities of Vietnamese edible plants and their relationships with polyphenol contents. J Nutr Sci Vitaminol. 2007, 53: 267-276.

Aurand LW, Boone NH, Giddings GG: Superoxide and singlet oxygen in milk lipid peroxidation. J Dairy Sci. 1977, 60: 363-369.

Halliwell B, Gutteridge JMC, Aruoma OI: The deoxyribose method: a simple 'test tube' assay for determination of rate constants for reaction of hydroxyl radicals. Anal Biochem. 1987, 165: 215-219.

Korycka-Dahl M, Richardson T: Photogeneration of superoxide anion in serum of bovine milk and in model systems containing riboflavin and amino acids. J Dairy Sci. 1978, 61: 400-407.

Robak J, Gryglewski IR: Flavonoids are scavengers of superoxide anions. Biochem Pharmacol. 1988, 37: 837-841.

Tylor BS, Kion YM, Wang QI, Sharpio RA, Billiar TR, Geller DA: Nitric oxide down regulates hepatocyte-inducible nitric oxide synthase gene expression. Arch Surg. 1997, 132: 1177-1183.

Huie RE, Padmaja S: The reaction of NO with superoxide. Free Radic Res Commun. 1993, 18: 195-199.

Miller MJ, Sadowska-Krowicka H, Chotinaruemol S, Kakkis JL, Clark DA: Amelioration of chronic ileitis by nitric oxide synthase inhibition. J Pharmacol Exp Ther. 1993, 264 (1): 11-16.

Balavoine GG, Geletti YV: Peroxynitrite scavenging by different antioxidants. Part 1: convenient study. Nitric oxide. 1999, 3: 40-54.

Ischiropoulos H, al-Mehdi AB, Fisher AB: Reactive species in ischemic rat lung injury: contribution of peroxynitrite. Am J Physiol. 1995, 269 (2 Pt 1): L158-L164.

Kochevar EI, Redmond WR: Photosnsitized production of singlet oxygen. Methods Enzymol. 2000, 319: 20-28.

Aruoma OI, Halliwell B, Hoey BM, Butler J: The antioxidant action of N-acetylcysteine: Its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Rad Biol Med. 1989, 6: 593-597.

Halliwell B: Reactive oxygen species in living systems: source, biochemistry, and role in human disease. Am J Med. 1991, 91 (3C): 14S-22S.

Duh PD, Tu YY, Yen GC: Antioxidant activity of water extract of Harng Jyur (Chrysenthemum morifolium Ramat). Lebnes wiss Technol. 1999, 32: 269-277.

Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF, Bilaloglu V: Comparison of antioxidant and antimicrobial activities of Tilia (Tilia argentea Desf Ex DC), Sage (Savia triloba L.), and Black Tea (Camellia sinensis) extracts. J Agric Food Chem. 2000, 48 (10): 5030-5034.

Cook NC, Samman S: Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. J Nutr Biochem. 1996, 7: 66-76.

Thông tin
Thông tin xuất bản

BMC Complementary and Alternative Medicine

Tập 8 Số 1

Thông tin tác giả