Hoạt động chống oxy hóa và quét gốc tự do của Spondias pinnata
Tóm tắt
Nhiều bệnh lý liên quan đến stress oxy hóa do các gốc tự do gây ra. Nghiên cứu hiện tại hướng đến việc tìm kiếm các chất chống oxy hóa có nguồn gốc tự nhiên từ thực vật. Mục tiêu của nghiên cứu này là đánh giá hoạt động chống oxy hóa
Chiết xuất methanol 70% từ vỏ thân cây
Chiết xuất này cho thấy hoạt động chống oxy hóa tổng thể với giá trị nồng độ chống oxy hóa tương đương trolox (TEAC) là 0.78 ± 0.02. Các giá trị IC50 cho khả năng quét các gốc tự do lần lượt là 112.18 ± 3.27 μg/ml, 13.46 ± 0.66 μg/ml và 24.48 ± 2.31 μg/ml đối với hydroxyl, superoxide và nitric oxide. Giá trị IC50 cho khả năng quét hydrogen peroxide là 44.74 ± 25.61 mg/ml. Đối với các hoạt động quét peroxynitrite, oxy đơn và acid hypochlorous, các giá trị IC50 lần lượt là 716.32 ± 32.25 μg/ml, 58.07 ± 5.36 μg/ml và 127.99 ± 6.26 μg/ml. Chiết xuất cũng được tìm thấy là chất chelat sắt mạnh với IC50 = 66.54 ± 0.84 μg/ml. Sức mạnh khử tăng lên khi lượng chiết xuất tăng. Chiết xuất thực vật (100 mg) tạo ra 91.47 ± 0.004 mg/ml hàm lượng phenolic tương đương acid gallic và 350.5 ± 0.004 mg/ml hàm lượng flavonoid tương đương quercetin.
Nghiên cứu này cung cấp chứng cứ rằng chiết xuất methanol 70% từ vỏ thân cây
Từ khóa
Tài liệu tham khảo
Braca A, Sortino C, Politi M, Morelli I, Mendez J: Antioxidant activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol. 2002, 79: 379-381.
Niki E, Shimaski H, Mino M: Antioxidantism-free radical and biological defense. Gakkai Syuppn Center, Tokyo. 1994, 3-16.
Tandon S, Rastogi RP: Studies on the chemical constituents of Spondias pinnata. Planta Med. 1976, 29: 190-192.
Ghosal PK, Thakur S: Structural features of the acidic polysaccharide of Spondias pinnata gum exudates. Carbohydr Res. 1981, 98: 75-83.
Bibitha B, Jisha VK, Salitha CV, Mohan S, Valsa AK: Antibacterial activity of different plant extracts. Indian J Microbiol. 2002, 42 (4): 361-363.
Mahanta RK, Rout SD, Sahu HK: Ethnomedicinal plant resources of Similipal biosphere reserve, Orissa, India. Zoos Print J. 2006, 21 (8): 2372-2374.
Leksomboon C, Thaveechai N, Kositratana W: Potential of plant extracts for controlling citrus canker of lime. Kasetsart J (Nat Sci). 2001, 35: 392-396.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C: Antioxidant activity applying an improved ABTS radical cation decolorization asszy. Free Rad Biol Med. 1999, 26: 1231-1237.
Elizabeth K, Rao MNA: Oxygen radical scavenging activity of curcumin. Int J Pharmaceut. 1990, 58: 237-240.
Fontana M, Mosca L, Rosei MA: Interaction of enkephalines with oxyradicals. Biochem Pharmacol. 2001, 61: 1253-1257.
Garratt DC: The quantitative analysis of Drugs. 1964, Chapman and Hall ltd, Japan, 3: 456-458.
Long LH, Evans PJ, Halliwell B: Hydrogen peroxide in human urine: implications for antioxidant defense and redox regulation. Biochem Biophys Res Commun. 1999, 262: 605-609.
Floriano-Sánchez E, Villanueva C, Medina-Campos ON, Rocha D, Sánchez-González DJ, Cárdenas-Rodríguez N, Pedraza-Chaverrí J: Nordihydroguaiaretic acid is a potent in vitro scavenger of peroxynitrite, singlet oxygen, hydroxyl radical, superoxide anion, andhypochlorous acid and prevents in vivo tyrosine nitration in lung. Free Radic Res. 2006, 40: 523-533.
Beckman JS, Chen H, Ischiropulos H, Crow JP: Oxidative chemistry of peroxynitrite. Methods Enzymol. 1994, 233: 229-240.
Bailly F, Zoete V, Vamecq J, Catteu JP, Bernier JL: Antioxidant actions of ovothiol-derived 4-mercaptoimidazoles: glutathione peroxidase activity and protection against peroxynitrite-induced damage. FEBS Lett. 2000, 486: 19-22.
Chakraborty N, Tripathy BC: Involvement of singlet oxygen in 5-aminolevulinic acid-induced photodynamic damage of cucumber (Cucumbis sativus L.) chloroplasts. Plant Physiol. 1992, 98: 7-11.
Pedraza-Chaverrí J, Barrera D, Maldonado PD, Chirino YI, Macías-Ruvalcaba NA, Medina-Campos ON, Castro L, Salcedo MI, Hernández-Pando R: S-allylmercaptocysteine scavenges hydroxyl radical and singlet oxygen in vitro and attenuates gentamicininduced oxidative and nitrosative stress and renal damage in vivo. BMC Clin Pharmacol. 2004, 4: 5-
Aruoma OI, Halliwell B: Action of hypochlorous acid on the antioxidant protective enzymes superoxide dismutase, catalase and glutathione peroxidase. Biochem J. 1987, 248: 973-976.
Pedraza-Chaverrí J, Arriaga-Noblecía G, Medina-Campos ON: Hypochlorous acid scavenging capacity of garlic. Phytother Res. 2007, 21: 884-888.
Haro-Vicente JF, Martinez-Gracia C, Ros G: Optimization of in vitro measurement of available iron from different fortificants in citric fruit juices. Food Chem. 2006, 98: 639-648.
Oyaizu M: Studies on products of browning reactions: antioxidant activities of products of browning reaction prepared from glucose amine. Jap J Nutr. 1986, 44: 307-315.
Singleton VL, Rossi JA: Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic. 1965, 16: 144-158.
Zhishen J, Mengcheng T, Jianming W: The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999, 64: 555-559.
Halliwell B, Gutteridge JM: Free radicals in biology and medicine. 1998, Oxford: Oxford University Press
Yazdanparast R, Ardestani A: In vitro antioxidant and free radical scavenging activity of Cyperus rotundus. J Med Food. 2007, 10: 667-674.
Yazdanparast R, Bahramikias S, Ardestani A: Nasturtium oficinale reduces oxidative stress and enhances antioxidant capacity in hypercholesterolaemic rats. Chem Biol Interact. 2008, 172: 176-184.
Packer L, Ong ASH: Biological oxidants and antioxidants: Molecular mechanisms and health effects. 1997, Champaign, IL: AOCS Press
Mai TT, Thu NN, Tien PG, Chuyen NV: Alpha-Glucosidase inhibitory and antioxidant activities of Vietnamese edible plants and their relationships with polyphenol contents. J Nutr Sci Vitaminol. 2007, 53: 267-276.
Aurand LW, Boone NH, Giddings GG: Superoxide and singlet oxygen in milk lipid peroxidation. J Dairy Sci. 1977, 60: 363-369.
Halliwell B, Gutteridge JMC, Aruoma OI: The deoxyribose method: a simple 'test tube' assay for determination of rate constants for reaction of hydroxyl radicals. Anal Biochem. 1987, 165: 215-219.
Korycka-Dahl M, Richardson T: Photogeneration of superoxide anion in serum of bovine milk and in model systems containing riboflavin and amino acids. J Dairy Sci. 1978, 61: 400-407.
Robak J, Gryglewski IR: Flavonoids are scavengers of superoxide anions. Biochem Pharmacol. 1988, 37: 837-841.
Tylor BS, Kion YM, Wang QI, Sharpio RA, Billiar TR, Geller DA: Nitric oxide down regulates hepatocyte-inducible nitric oxide synthase gene expression. Arch Surg. 1997, 132: 1177-1183.
Miller MJ, Sadowska-Krowicka H, Chotinaruemol S, Kakkis JL, Clark DA: Amelioration of chronic ileitis by nitric oxide synthase inhibition. J Pharmacol Exp Ther. 1993, 264 (1): 11-16.
Balavoine GG, Geletti YV: Peroxynitrite scavenging by different antioxidants. Part 1: convenient study. Nitric oxide. 1999, 3: 40-54.
Ischiropoulos H, al-Mehdi AB, Fisher AB: Reactive species in ischemic rat lung injury: contribution of peroxynitrite. Am J Physiol. 1995, 269 (2 Pt 1): L158-L164.
Kochevar EI, Redmond WR: Photosnsitized production of singlet oxygen. Methods Enzymol. 2000, 319: 20-28.
Aruoma OI, Halliwell B, Hoey BM, Butler J: The antioxidant action of N-acetylcysteine: Its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Rad Biol Med. 1989, 6: 593-597.
Halliwell B: Reactive oxygen species in living systems: source, biochemistry, and role in human disease. Am J Med. 1991, 91 (3C): 14S-22S.
Duh PD, Tu YY, Yen GC: Antioxidant activity of water extract of Harng Jyur (Chrysenthemum morifolium Ramat). Lebnes wiss Technol. 1999, 32: 269-277.
Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF, Bilaloglu V: Comparison of antioxidant and antimicrobial activities of Tilia (Tilia argentea Desf Ex DC), Sage (Savia triloba L.), and Black Tea (Camellia sinensis) extracts. J Agric Food Chem. 2000, 48 (10): 5030-5034.