Antimicrobial effects of mustard oil-containing plants against oral pathogens: an in vitro study
Tóm tắt
The present study examines the antimicrobial activity of nasturtium herb (Tropaeoli maji herba) and horseradish root (Armoraciae rusticanae radix) against clinically important oral bacterial pathogens involved in periodontitis, gingivitis, pulpitis, implantitis and other infectious diseases. A total of 15 oral pathogens, including members of the genera Campylobacter, Fusobacterium, Prevotella, Parvimonas, Porphyromonas, Tanerella, Veillonella, and HACEK organisms, were exposed to [1] a combination of herbal nasturtium and horseradish using a standardized gas test and [2] a mixture of synthetic Isothiocyantes (ITCs) using an agardilution test. Headspace gas chromatography mass spectrometry was employed to quantify the amount of allyl-, benzyl-, and 2- phenyl- ethyl-ITC. With exception of Veillonella parvula, all tested species were highly susceptible to herbal nasturtium and horseradish in the gas test with minimal inhibitory concentrations (MICs) between 50/20 mg and 200/80 mg and to synthetic ITCs in the agardilution with MICs between 0.0025 and 0.08 mg ITC/mL, respectively. Minimal bactericidal concentrations extended from 0.005 mg ITC/mL to 0.34 mg ITC/mL. ITCs may be considered an interesting alternative to antibiotics for prevention and treatment of oropharyngeal infections, periodontitis and related diseases. Furthermore, the suitability of ITCs for endocarditis prophylaxis in dental procedures might be worth further investigation.
Tài liệu tham khảo
Aminov RI. A brief history of the antibiotic era: lessons learned and challenges for the future. Front Microbiol. 2010;1:134.
Renwick MJ, Simpkin V, Mossialos E. European Observatory Health Policy Series. Targeting innovation in antibiotic drug discovery and development: The need for a One Health - One Europe - One World Framework. Copenhagen (Denmark): European Observatory on Health Systems and Policies World Health Organization 2016; 2016.
Laxminarayan R, Matsoso P, Pant S, Brower C, Rottingen JA, Klugman K, et al. Access to effective antimicrobials: a worldwide challenge. Lancet (London, England). 2016;387(10014):168–75.
Lewis K. Antibiotics: recover the lost art of drug discovery. Nature. 2012;485(7399):439–40.
Meyer B, Cookson B. Does microbial resistance or adaptation to biocides create a hazard in infection prevention and control? J Hospital Infect. 2010;76(3):200–5.
Kampf G. Acquired resistance to chlorhexidine - is it time to establish an ‘antiseptic stewardship’ initiative? J Hospital Infect. 2016;94(3):213–27.
Horner C, Mawer D, Wilcox M. Reduced susceptibility to chlorhexidine in staphylococci: is it increasing and does it matter? J Antimicrob Chemother. 2012;67(11):2547–59.
Dufour V, Stahl M, Baysse C. The antibacterial properties of isothiocyanates. Microbiology (Reading, England). 2015;161(Pt 2):229–43.
Benyelles B, Allali H, Fekih N, Touaibia M, Muselli A, Nassim D, et al. Chemical Composition of the Volatile Components of Tropaeolum majus L. (Garden Nasturtium) from North Western Algeria. Phyto Chem Bio Sub J. 2015;9.
Conrad A, Biehler D, Nobis T, Richter H, Engels I, Biehler K, et al. Broad spectrum antibacterial activity of a mixture of isothiocyanates from nasturtium (Tropaeoli majoris herba) and horseradish (Armoraciae rusticanae radix). Drug Res. 2013;63(2):65–8.
Mutters NT, Mampel A, Kropidlowski R, Biehler K, Gunther F, Balu I, et al. Treating urinary tract infections due to MDR E. coli with Isothiocyanates - a phytotherapeutic alternative to antibiotics? Fitoterapia. 2018;129:237–40.
Kaiser SJ, Mutters NT, Blessing B, Gunther F. Natural isothiocyanates express antimicrobial activity against developing and mature biofilms of Pseudomonas aeruginosa. Fitoterapia. 2017;119:57–63.
Romeo L, Iori R, Rollin P, Bramanti P, Mazzon E. Isothiocyanates: An Overview of Their Antimicrobial Activity against Human Infections. Molecules (Basel, Switzerland). 2018;23(3).
Aires A, Mota VR, Saavedra MJ, Rosa EAS, Bennett RN. The antimicrobial effects of glucosinolates and their respective enzymatic hydrolysis products on bacteria isolated from the human intestinal tract. J Appl Microbiol. 2009;106(6):2086–95.
Goos KH, Albrecht U, Schneider B. Efficacy and safety profile of a herbal drug containing nasturtium herb and horseradish root in acute sinusitis, acute bronchitis and acute urinary tract infection in comparison with other treatments in the daily practice/results of a prospective cohort study. Arzneimittelforschung. 2006;56(3):249–57.
Goos KH, Albrecht U, Schneider B. On-going investigations on efficacy and safety profile of a herbal drug containing nasturtium herb and horseradish root in acute sinusitis, acute bronchitis and acute urinary tract infection in children in comparison with other antibiotic treatments. Arzneimittelforschung. 2007;57(4):238–46.
Fintelmann V, Albrecht U, Schmitz G, Schnitker J. Efficacy and safety of a combination herbal medicinal product containing Tropaeoli majoris herba and Armoraciae rusticanae radix for the prophylactic treatment of patients with respiratory tract diseases: a randomised, prospective, double-blind, placebo-controlled phase III trial. Curr Med Res Opin. 2012;28(11):1799–807.
Albrecht U, Goos KH, Schneider B. A randomised, double-blind, placebo-controlled trial of a herbal medicinal product containing Tropaeoli majoris herba (Nasturtium) and Armoraciae rusticanae radix (horseradish) for the prophylactic treatment of patients with chronically recurrent lower urinary tract infections. Curr Med Res Opin. 2007;23(10):2415–22.
CLSI. Performance Standards for Antimicrobial Disk Susceptibility Tests, Approved Standard, CLSI document M02-A11. 7th ed. Pennsylvania 19087, USA: Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne; 2012.
Winter AG, Hornbostel M. Untersuchungen über Antibiotika aus höheren Pflanzen: IX. Mitteilung: Gasförmige Hemmstoffe aus Cochlearia armoracia (Meerrettich) und ihr Verhalten im menschlichen Körper bei Aufnahme per os. Naturwiss. 1953;40:489–90.
Park H-W, Choi K-D, Shin I-S. Antimicrobial activity of Isothiocyanates extracted from horseradish root against Oral microorganisms. Biocontrol Science. 2013;18(3):163–8.
Sofrata A, Santangelo EM, Azeem M, Borg-Karlson A-K, Gustafsson A, Pütsep K. Benzyl Isothiocyanate, a major component from the roots of Salvadora Persica is highly active against gram-negative Bacteria. PLoS One. 2011;6(8):e23045.
Quirynen M, Avontroodt P, Peeters W, Pauwels M, Coucke W, van Steenberghe D. Effect of different chlorhexidine formulations in mouthrinses on de novo plaque formation. J Clin Periodontol. 2001;28(12):1127–36.
John G, Becker J, Schwarz F. Effects of Taurolidine and Chlorhexidine on SaOS-2 cells and human gingival fibroblasts grown on implant surfaces. The international journal of Oral &. Maxillofacial Implants. 2014;29(3):728–34.
Proksch S, Strobel SL, Vach K, Abouassi T, Tomakidi P, Ratka-Kruger P, et al. Melatonin as a candidate therapeutic drug for protecting bone cells from chlorhexidine-induced damage. J Periodontol. 2014;85(12):e379–89.
Abouassi T, Hannig C, Mahncke K, Karygianni L, Wolkewitz M, Hellwig E, et al. Does human saliva decrease the antimicrobial activity of chlorhexidine against oral bacteria? BMC Res Notes. 2014;7:711.
Cieplik F, Jakubovics NS, Buchalla W, Maisch T, Hellwig E, Al-Ahmad A. Resistance toward Chlorhexidine in Oral Bacteria - is there cause for concern? Front Microbiol. 2019;10:587.
Vlachojannis C, Chrubasik-Hausmann S, Hellwig E, Al-Ahmad A. A preliminary investigation on the antimicrobial activity of Listerine(R), its components, and of mixtures thereof. Phytotherapy Res PTR. 2015;29(10):1590–4.
Vlachojannis C, Al-Ahmad A, Hellwig E, Chrubasik S. Listerine(R) products: an update on the efficacy and safety. Phytotherapy Res PTR. 2016;30(3):367–73.
Könönen E, Müller H-P. Microbiology of aggressive periodontitis. Periodontol. 2014;65(1):46–78.
Picolos DK, Lerche-Sehm J, Abron A, Fine JB, Papapanou PN. Infection patterns in chronic and aggressive periodontitis. J Clin Periodontol. 2005;32(10):1055–61.
Cahill TJ, Prendergast BD. Infective endocarditis. Lancet (London, England). 2016;387(10021):882–93.