Antimicrobial activity of platelet‐leukocyte gel against Staphylococcus aureus

Journal of Orthopaedic Research - Tập 26 Số 3 - Trang 404-410 - 2008
Dirk Jan F Moojen1, Peter A. Everts2, Rose‐Minke Schure1, E.P. Overdevest2, André van Zundert3, Johannes T. A. Knape4, René M. Castelein1, Laura B. Creemers1, Wouter J.A. Dhert1
1Department of Orthopaedics, University Medical Center Utrecht, Room G05.228, P.O. Box 85500, 3508 GA Utrecht, The Netherlands.
2Department of Blood Management, Catharina Hospital Eindhoven, Eindhoven, The Netherlands
3Department of Anesthesiology, Catharina Hospital Eindhoven, Eindhoven, The Netherlands
4Department of Anesthesiology and Perioperative Care, University Medical Center Utrecht, Utrecht, The Netherlands

Tóm tắt

AbstractPlatelet‐leukocyte gel (PLG) contains high concentrations of platelets and leukocytes. As leukocytes play an important role in the innate host‐defense, we hypothesized that PLG might have antimicrobial properties. This study investigated the antimicrobial activity of PLG against Staphylococcus aureus and the contribution of myeloperoxidase (MPO), present in leukocytes, in this process. Platelet‐rich plasma (PRP) and platelet‐poor plasma (PPP) were obtained from whole blood of six donors. PLG was prepared by mixing PRP with autologous (PLG‐AT) or bovine thrombin (PLG‐BT). Antimicrobial activity of PLG‐AT, PLG‐BT, PRP, and PPP was determined in a bacterial kill assay. MPO release was measured by ELISA and activity was measured using a MPO activity assay. Cultures showed a rapid decrease in the number of bacteria for both PLG‐AT and PLG‐BT, which was maximal between 4 and 8 h, to approximately 1% of the bacteria in controls. The effect of PLG‐AT was largest and significantly different compared to PRP (p = 0.004) and PPP (p < 0.001), however not compared to PLG‐BT (p = 0.093). PLG‐AT, PLG‐BT, and PRP showed a comparable, gradually increasing MPO release. MPO activity was comparable for all groups and remained stable. No correlation between MPO release, activity, and bacterial kill could be found. PLG appears to have potent antimicrobial capacity, but the role of MPO in this activity is questionable. PLG might represent a useful strategy against postoperative infections. However, additional research should elucidate its exact antimicrobial activity. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:404–410, 2008

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Tài liệu tham khảo

10.1177/154405910508400507

10.2337/diacare.24.3.483

10.1111/j.1537-2995.2004.03366.x

Rughetti A, 2004, Closed surgery: autologous platelet gel for the treatment of pseudoarthrosis, Blood Transfus, 2, 37

Bibbo C, 2005, Union rates using autologous platelet concentrate alone and with bone graft in high‐risk foot and ankle surgery patients, J Surg Orthop Adv, 14, 17

10.1016/S1079-2104(98)90029-4

10.1111/j.1399-6576.2006.001005.x

10.1007/s00264-006-0174-z

10.1111/j.1365-3148.2006.00708.x

10.1002/jor.1100130504

10.1016/j.transci.2004.01.004

10.1089/ten.2006.12.3067

10.1016/j.orthres.2004.08.015

10.1902/jop.2007.060302

10.1074/jbc.275.27.20374

10.1128/IAI.64.9.3512-3517.1996

10.1089/10799900260286623

10.1128/IAI.70.12.6524-6533.2002

10.1302/0301-620X.89B3.18491

10.1007/s00586-005-0962-y

10.1093/infdis/146.4.487

10.1002/1097-4636(20001215)52:4<754::AID-JBM21>3.0.CO;2-#

10.1002/jor.1100180323

10.1002/jor.1100120316

10.1074/jbc.273.9.4997

10.1128/IAI.63.8.3042-3047.1995

10.1182/blood.V92.9.3007

10.1056/NEJMoa035003

10.1016/S1074-5521(02)00149-7

Qian M, 1997, Cyanate‐mediated inhibition of neutrophil myeloperoxidase activity, Biochem J, 326, 159, 10.1042/bj3260159

10.1042/bj3080559

10.1086/516120

Trowbridge CC, 2005, Use of platelet gel and its effects on infection in cardiac surgery, J Extra Corpor Technol, 37, 381, 10.1051/ject/200537381

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Journal of Orthopaedic Research

Tập 26 Số 3

404-410

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