Antimicrobial Activity of Culture Filtrate of Bacillus amyloliquefaciens RC-2 Isolated from Mulberry Leaves

Phytopathology - Tập 91 Số 2 - Trang 181-187 - 2001
Shigenobu Yoshida1, Syuntaro Hiradate1, T Tsukamoto1, Kiyotaka Hatakeda1, Akira Shirata1
1National Institute for Agro-Environmental Sciences

Tóm tắt

A potential antagonist, Bacillus amyloliquefaciens strain RC-2, against Colletotrichum dematium, mulberry anthracnose fungus, was obtained from healthy mulberry leaves by in vitro and in vivo screening techniques. Application of culture filtrate of RC-2 inhibited disease on mulberry leaves, indicating that suppression was due to antifungal compounds in the filtrate. Development of mulberry anthracnose on mulberry leaves was inhibited only when the culture filtrate was applied before fungal inoculation, and it was not inhibited by application after inoculation. These results suggest that the antifungal compounds in the filtrate exhibit a preventive effect on the disease. Peptone significantly increased production of the antifungal compounds. The culture filtrate of RC-2 also inhibited the growth of several other phytopathogenic fungi and bacteria, such as Rosellinia necatrix, Pyricularia oryzae, Agrobacterium tumefaciens, and Xanthomonas campestris pv. campestris, in vitro. From the culture filtrate of RC-2, seven kinds of antifungal compounds were isolated by high performance liquid chromatography analysis, and one of the compounds was determined as iturin A2, a cyclic peptide, by nuclear magnetic resonance and fast atom bombardment mass analysis.

Từ khóa


Tài liệu tham khảo

10.1146/annurev.py.06.090168.001403

10.1080/07060668609501829

10.1016/S0944-5013(11)80064-9

10.1002/bscb.19650740704

10.7202/706018ar

10.1146/annurev.py.26.090188.000451

Gross E., 1973, Z. Physiol. Chem., 354, 810

10.1016/S0040-4039(00)87534-6

10.3186/jjphytopath.62.559

10.1071/APP9940041

Kimura, K. 1979. The Illustration of Mulberry Diseases in Japan. Kenpakusya, Tokyo.

Logan, N. A., and Berkeley, R. C. W. 1981. Classification and identification of members of the genus Bacillus using API tests. Pages 105-140 in: The Aerobic Endospore-Forming Bacteria: Classification and Identification. R. C. W. Berkeley and M. Goodfellow, eds. Academic Press, London.

Logan N. A., 1984, J. Gen. Microbiol., 130, 1871

10.1006/bcon.1996.0060

10.1099/00207713-30-2-448

10.3186/jjphytopath.58.329

10.1139/m96-072

Ryu E., 1940, Kitasato Arch. Exp. Med., 17, 58

10.1042/bj1210839

Slepecky, R. A., and Hemphill, H. E. 1992. The genus Bacillusnonmedical. Pages 1687-1688 in: The Prokaryotes, 2nd ed. A. Balows, H. G. Truper, M. Dworkin, W. Harder, and K. H. Schleifer, eds. Springer-Verlag, New York.

Sneath, P. H. A. 1986. Endospore-forming gram-positive rods and cocci. Pages 1104-1138 in: Bergey's Manual of Systematic Bacteriology, Vol. 2. P. H. A. Sneath, ed. Williams and Wilkins, Baltimore, MD.

10.1111/j.1744-7348.1978.tb02572.x

10.1139/m92-209

10.1042/bj1180557

10.1046/j.1365-2672.1998.00411.x

Welker N. E., 1967, J. Bacteriol., 94, 1124, 10.1128/jb.94.4.1124-1130.1967

Yoshida S., 1997, Bull. Natl. Inst. Seric. Entomol. Sci., 18, 79

Yoshida S., 1998, J. Seric. Sci. Jpn., 67, 327

Yoshida, S., and Shirata, A. 1998. Mulberry anthracnose. Page 195 in: Plant Diseases in Japan. K. Kishi, ed. Zenkoku Nouson Kyouiku Kyoukai, Tokyo.

10.1094/PDIS.1999.83.5.465

10.3186/jjphytopath.61.75

Yu, G. Y., and Sinclair, J. B. 1996. Evaluation of Bacillus amyloliquefaciens B94 for control of Rhizoctonia seedling disease on soybeans. (Abstr.) Phytopathology 86(suppl.):S54.

Thông tin
Thông tin xuất bản

Phytopathology

Tập 91 Số 2

181-187

Thông tin tác giả