Antigen processing and CD24 expression determine antigen presentation by splenic CD4+ and CD8+ dendritic cells

Immunology - Tập 123 Số 3 - Trang 447-455 - 2008
David Askew1, Clifford V. Harding2
1Department of Dermatology, Case Western Reserve University, Cleveland, OH 44106 USA
2Department of Pathology, Case Western Reserve University, Cleveland, OH, USA

Tóm tắt

SummaryTo examine heterogeneity in dendritic cell (DC) antigen presentation function, murine splenic DCs were separated into CD4+ and CD8+ populations and assessed for the ability to process and present particulate antigen to CD4+ and CD8+ T cells. CD4+ and CD8+ DCs both processed exogenous particulate antigen, but CD8+ DCs were much more efficient than CD4+ DCs for both major histocompatibility complex (MHC) class II antigen presentation and MHC class I cross‐presentation. While antigen processing efficiency contributed to the superior antigen presentation function of CD8+ DCs, our studies also revealed an important contribution of CD24. CD8+ DCs were also more efficient than CD4+ DCs in inducing naïve T cells to acquire certain effector T‐cell functions, for example generation of cytotoxic CD8+ T cells and interferon (IFN)‐γ‐producing CD4+ T cells. In summary, CD8+ DCs are particularly potent antigen‐presenting cells that express critical costimulators and efficiently process exogenous antigen for presentation by both MHC class I and II molecules.

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Immunology

Tập 123 Số 3

447-455

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