Antigen processing and CD24 expression determine antigen presentation by splenic CD4+ and CD8+ dendritic cells
Tóm tắt
To examine heterogeneity in dendritic cell (DC) antigen presentation function, murine splenic DCs were separated into CD4+ and CD8+ populations and assessed for the ability to process and present particulate antigen to CD4+ and CD8+ T cells. CD4+ and CD8+ DCs both processed exogenous particulate antigen, but CD8+ DCs were much more efficient than CD4+ DCs for both major histocompatibility complex (MHC) class II antigen presentation and MHC class I cross‐presentation. While antigen processing efficiency contributed to the superior antigen presentation function of CD8+ DCs, our studies also revealed an important contribution of CD24. CD8+ DCs were also more efficient than CD4+ DCs in inducing naïve T cells to acquire certain effector T‐cell functions, for example generation of cytotoxic CD8+ T cells and interferon (IFN)‐γ‐producing CD4+ T cells. In summary, CD8+ DCs are particularly potent antigen‐presenting cells that express critical costimulators and efficiently process exogenous antigen for presentation by both MHC class I and II molecules.
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Tài liệu tham khảo
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