Antigen presentation by macrophages is enhanced by the uptake of necrotic, but not apoptotic, cells

Clinical and Experimental Immunology - Tập 127 Số 2 - Trang 220-225 - 2002
Robert N. Barker, L-P Erwig, Kathryn Hill1, Anne Devine1, Wayne Pearce1, Andrew J. Rees1
1Department of Medicine and Therapeutics, University of Aberdeen, UK

Tóm tắt

SummaryThe aim of this study was to determine whether phagocytosis of necrotic or apoptotic cells affects antigen presentation by murine bone marrow-derived macrophages. After uptake of necrotic neutrophils, macrophages were able to stimulate significantly higher T cell proliferation in vitro against both the recall antigen albumin and the mitogen concanavalin A. No such effect was seen following phagocytosis of apoptotic neutrophils. Flow cytometry revealed that, within 4h of ingestion, macrophages that had taken up the necrotic cells expressed higher levels of CD40 than those that had phagocytosed apoptotic cells. Macrophage cultures pulsed with apoptotic, but not necrotic, neutrophils contained higher levels of transforming growth factor β1, but lower concentrations of tumour necrosis factor α, compared to untreated controls. Our interpretation of these results is that macrophages that have taken up necrotic neutrophils co-stimulate T cells with greater efficiency due to rapid CD40 up-regulation, whereas those that have ingested apoptotic cells are not only ineffective in co-stimulation, but also secrete inhibitory cytokine.

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Clinical and Experimental Immunology

Tập 127 Số 2

220-225

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