Antifungal effects of seven plant essential oils against Penicillium digitatum
Tóm tắt
Research interest in plant essential oils has increased significantly due to their natural properties and consumer demand for safer methods of food preservation. Plants produce large amounts of secondary metabolites, which have potential activity against fungal pathogens. This study aimed at screening essential oils for their antifungal effects on citrus against Penicillium digitatum, morphological effect and finally determine which essential oils are the most effective. The EC50 of seven selected cinnamon (0.424 μL/mL), patchouli (0.513 μL/mL), vetiver (0.612 μL/mL), dill (1.597 μL/mL), origanum (1.971 μL/mL) and ylang (2.214 μL/mL) was determined. In addition, cinnamon substantially reduced sporulation (100%) followed by patchouli (86.02%), vetiver (82.73%), and chamomile (79.04%), respectively. Our GC–MS result determined variance in concentration of essential oils compound composition. The total compound composition in all seven essential oils > 1% was found to be 3 in cinnamon, 5 in dill, 10 in origanum, 13 in ylang, 11 in patchouli, 9 in chamomile and 16 in vetiver. Addition of essential oils significantly altered fungal morphology by scanning electron cryomicroscopy. Patchouli and origanum showed broken hyphae while there was an indication of severe deformation and collapse of spores in cinnamon and chamomile. Based on our findings, we report that these essential oils could potentially be applicable in controlling P. digitatum with reduced concern for human health, environmental contamination and possibly replacement of synthetic treatments.
Tài liệu tham khảo
Wu GA, Terol J, Ibanez V. Genomics of the origin and evolution of citrus. Nature. 2018;554(7692):311–6. https://doi.org/10.1038/nature25447.
Fadli A, Benyahia H, Hussain S, Khan RI, Rao MJ, Ahmed T, Ancona V, Khalid MF. Phytophthora-citrus interactions and management strategies: a review. Turk J Agric For. 2022;46(5):730–42. https://doi.org/10.55730/1300-011X.3038.
Wuryatmo E, Klieber A, Scott ES. Inhibition of citrus post-harvest pathogens by vapor of citral and related compounds in culture. J Agric Food Chem. 2003;51:2637–40. https://doi.org/10.1021/jf026183l.
Mahawer AK, Dubey AK, Awasthi OP, Singh D, Dahuja A, Sevanthi AM, Kumar A, Goswami AK, Sharma N, Yadav J, Kesharwani AK. Elucidation of physio-biochemical changes in Citrus spp. incited by Xanthomonas citri pv. citri. J Hortic. 2023;9(3):324. https://doi.org/10.3390/horticulturae9030324.
Marcet-Houben M, Ana-Rosa B, Beatriz DF, Eleonora H, Jose FM, Luis GC, Tony G. Genome sequence of the necrotrophic fungus Penicillium digitatum, the main post-harvest pathogen of citrus. BMC Genom. 2012;13(1):1–18. https://doi.org/10.1186/1471-2164-13-646.
Narware J, Singh SP, Manzar N, Kashyap AS. Biogenic synthesis, characterization, and evaluation of synthesized nanoparticles against the pathogenic fungus Alternaria solani. Front Microbiol. 2023;14:1159251. https://doi.org/10.3389/fmicb.2023.1159251.
Kashyap AS, Manzar N, Rajawat MVS, Kesharwani AK, Singh RP, Dubey SC, Pattanayak D, Dhar S, Lal SK, Singh D. Screening and biocontrol potential of rhizobacteria native to gangetic plains and hilly regions to induce systemic resistance and promote plant growth in chilli against bacterial wilt disease. Plants. 2021;10(10):2125. https://doi.org/10.3390/plants10102125.
Felšöciová S, Vukovic N, Jezowski P, Kacaniova M. Antifungal activity of selected volatile essential oils against Penicillium sp. Open Life Sci. 2020;15(1):511–21. https://doi.org/10.1515/biol-2020-0045.
Nabila EA, Soufiyan EA. Use of plant extracts in the control of post-harvest fungal rots in apples. J Bot Res. 2019;1(3):27–41. https://doi.org/10.30564/jrb.v1i3.1563.
Kordali S, Usanmaz Bozhuyuk A, Komaki A, Ilhan G, Ercisli S. Biological control of Penicillium on lemon fruits by essential oils of Satureja species. Erwerbs-obstbau. 2022;64:703–15. https://doi.org/10.1007/s10341-022-00686-7.
Kumar S, Korra T, Thakur R, Arutselvan R, Kashyap AS, Nehela Y, Chaplygin V, Minkina T, Keswani C. Role of plant secondary metabolites in defence and transcriptional regulation in response to biotic stress. J Stress. 2023. https://doi.org/10.1016/j.stress.2023.100154.
Kashyap AS, Manzar N, Nebapure SM, Rajawat MVS, Deo MM, Singh JP, Kesharwani AK, Singh RP, Dubey SC, Singh D. Unraveling microbial volatile elicitors using a transparent methodology for induction of systemic resistance and regulation of antioxidant genes at expression levels in chili against bacterial wilt disease. Antioxidants. 2022;11(2):404. https://doi.org/10.3390/antiox11020404.
Yin G, Yuliang Z, Kayla KP, Guangxi W, Sui Sheng TH, Jiujiang Y, Wayne MJ, Anping G, Joan WB. Characterization of blue mold Penicillium species isolated from stored fruits using multiple highly conserved loci. J Fungi. 2017;3(1):12. https://doi.org/10.3390/jof3010012.
El-Kalamouni C, Petras RV, Bachar Z, Othmane M, Christine R, Thierry T. Antioxidant and antimicrobial activities of the essential oil of Achillea millefolium L. grown in France. Medicines. 2017;4(2):30. https://doi.org/10.3390/medicines4020030.
Merah O, Bouchra SA, Thierry T, Zeinab S, Muriel C, Sarah G, Phillippe E, Akram H. Biochemical composition of cumin seeds, and biorefining study. Biomolecules. 2020;10(7):1054. https://doi.org/10.3390/biom10071054.
Ahmad BS, Thierry T, Zeinab S, Akram H, Muriel C, Hussein K, Ali C, Othmane M. Fennel oil and by-products seed characterization and their potential applications. Ind Crops Prod. 2018;111:92–8. https://doi.org/10.1016/j.indcrop.2017.10.008.
Wang D, Wang G, Wang J, Zhai H, Xue X. Inhibitory effect and underlying mechanism of cinnamon and clove essential oils on Botryosphaeria dothidea and Colletotrichum gloeosporioides causing rots in postharvest bagging-free apple fruits. Front Microbiol. 2023;14:1109028. https://doi.org/10.3389/fmicb.2023.1109028.
Ayaz M, Ullah F, Sadiq A, Ullah F, Ovais M, Ahmed J, Devkota HP. Synergistic interactions of phytochemicals with antimicrobial agents: potential strategy to counteract drug resistance. Chem Bio Interact. 2019;308:294–303. https://doi.org/10.1016/j.cbi.2019.05.050.
Smilanick JL, Monir FM, Franka MG, David S. Control of citrus post-harvest green mold and sour rot by potassium sorbate combined with heat and fungicides. Postharvest Biol Technol. 2008;47(2):226–38. https://doi.org/10.1016/j.postharvbio.2007.06.020.
Nicolopoulou-Stamati P, Sotirios M, Chrysanthi K, Panagiotis S, Luc H. Chemical pesticides and human health: the urgent need for a new concept in agriculture. Front Public Health. 2016;4:148. https://doi.org/10.3389/fpubh.2016.00148.
Isman MB. Plant essential oils for pest and disease management. Crop Prot. 2000;19(8–10):603–8. https://doi.org/10.1016/S0261-2194(00)00079-X.
Hrustić J, Goran D, Ivana S, Mila G, Branka K, Aleksandra B, Brankica T. Monilinia spp. causing brown rot of stone fruit in Serbia. Plant Dis. 2015;99(5):709–17.
Pascual S, De Cal A, Melgarejo P. Induction of conidia production by Monilinia laxa on agar media by acetone. Phytopathology. 1990;80(5):494–6.
Talibi I, Karim H, Askarne L. Antifungal activity of aqueous and organic extracts of eight aromatic and medicinal plants against Geotrichum candidum, causal agent of citrus sour rot. Int J Agron Plant Prod. 2013;4:3510–21.
Jiang L. Comparison of disk diffusion, agar dilution, and broth microdilution for antimicrobial susceptibility testing of five chitosans. Baton Rouge: Louisiana State University and Agricultural & Mechanical College; 2011. https://doi.org/10.31390/gradschool_theses.727.
Sridhar SR, Rajagopal RV, Ramasamy R, Selladurai M, Srivinasan N. Antifungal activity of some essential oils. J Agric Food Chem. 2003;51(26):7596–9.
Liu Y, Zhiyi C, Yongfeng L, Xiaoyu W, Chuping L, Yafeng N, Kerong W. Enhancing bioefficacy of Bacillus subtilis with sodium bicarbonate for the control of ring rot in pear during storage. Biol Control. 2011;57(2):110–7. https://doi.org/10.1016/j.biocontrol.2011.01.008.
Gemeda N, Woldeamanuel Y, Asrat D, Debelle A. Effect of essential oils on Aspergillus spore germination, growth and mycotoxin production: a potential source of botanical food preservative. Asian Pac J Biomed. 2014;4:S373–81. https://doi.org/10.12980/APJTB.4.2014C857.
Messgo-Moumene S, Ying L, Kamilia B, Zahia H, Zouaoui B, Farid C. Antifungal power of Citrus essential oils against potato late blight causative agent. J Essent Oil Res. 2015;27(2):169–76. https://doi.org/10.1080/10412905.2014.982877.
Arrebola E, Dharini S, Romina B, Lise K. Combined application of antagonist Bacillus amyloliquefaciens and essential oils for the control of peach post-harvest diseases. Crop Prot. 2010;29(4):369–77. https://doi.org/10.1016/j.cropro.2009.08.001.
OuYang Q, Reymick O, Yipeng C, Nengguo T. Synergistic activity of cinnamaldehyde and citronellal against green mold in citrus fruit. Postharvest Biol Technol. 2020;162: 111095. https://doi.org/10.1016/j.postharvbio.2019.111095.
Yigit F, Özcan M, Akgül A. Inhibitory effect of some spice essential oils on Penicillium digitatum causing post-harvest rot in citrus. Grasas Aceites. 2000;51(4):237–40. https://doi.org/10.3989/gya.2000.v51.i4.417.
Hlebová M, Foltinova D, Veselenylova D, Medo J, Sramkova Z, Tancinova D, Mrkvova M, Hleba L. The vapor phase of selected essential oils and their antifungal activity in vitro and in situ against Penicillium commune, a common contaminant of cheese. Foods. 2022;11(21):3517. https://doi.org/10.3390/foods11213517.
Yu D, Wang J, Shao X, Xu F, Wang H. Antifungal modes of action of tea tree oil and its two characteristic components against Botrytis cinerea. J Appl Microbiol. 2015;119(5):1253–62. https://doi.org/10.1111/jam.12939.
Fathi Z, Abbas H, Youbert G, Ali A, Mohammad HM. The potential of thyme, clove, cinnamon and ajowan essential oils in inhibiting the growth of Botrytis cinerea and Monilinia fructicola. J Essent Oil Bear Pl. 2012;15(1):38–47. https://doi.org/10.1080/0972060X.2012.10644017.
Lazar-Baker E, Hetherington SD, Ku VV, Newman SM. Evaluation of commercial essential oil samples on the growth of post-harvest pathogen Monilinia fructicola (G. Winter) Honey. Lett Appl Microbiol. 2011;52(3):227–32. https://doi.org/10.1111/j.1472-765X.2010.02996.x.
Viuda-Martos M, Ruiz NY, Fernandez JL, Perez-Alvarez JA. Antifungal activities of thyme, clove and oregano essential oils. J Food Saf. 2007;27(1):91–101. https://doi.org/10.1111/j.1745-4565.2007.00063.x.
Elshafie HS, Emilia M, Ippolito C, Laura DM, Vincenzo DF. In vivo antifungal activity of two essential oils from Mediterranean plants against post-harvest brown rot disease of peach fruit. Ind Crops Prod. 2015;66:11–5. https://doi.org/10.1016/j.indcrop.2014.12.031.
Alcazar-Fuoli L, Mellado E. Ergosterol biosynthesis in Aspergillus fumigatus: its relevance as an antifungal target and role in antifungal drug resistance. Front Microbiol. 2013;3:439. https://doi.org/10.3389/fmicb.2012.00439.
Brochot A, Angele G, Laila H, Christine R. Antibacterial, antifungal, and antiviral effects of three essential oil blends. Microbiologyopen. 2017;6(4): e00459. https://doi.org/10.1002/mbo3.459.
Salem EA, Youssef K, Sanzani SM. Evaluation of alternative means to control post-harvest Rhizopus rot of peaches. Sci Hortic. 2016;198:86–90. https://doi.org/10.1016/j.scienta.2015.11.013.
Yahyazadeh M, Omidbaigi R, Zare R. Effect of some essential oils on mycelial growth of Penicillium digitatum Sacc. World J Microbiol Biotechnol. 2008;24(8):1445–50. https://doi.org/10.1007/s11274-007-9636-8.
Charai M, Mosaddak M, Faid M. Chemical composition and antimicrobial activities of two aromatic plants: Origanum majorana L. and O. compactum Benth. J Essent Oil Res. 1996;8(6):657–64. https://doi.org/10.1080/10412905.1996.9701036.