Anticancer bioactivity of zerumbone on pediatric rhabdomyosarcoma cells

Journal of Cancer Research and Clinical Oncology - Tập 149 - Trang 3313-3323 - 2022
Cristian Urla1, Matias Julian Stagno1, Jörg Fuchs1, Steven W. Warmann1, Evi Schmid1
1Department of Pediatric Surgery and Pediatric Urology, University Children’s Hospital of Tuebingen, Tuebingen, Germany

Tóm tắt

Natural products are generally regarded as safe and have been shown to mediate anticancer activities against a variety of cell types. Zerumbone is a natural cyclic sesquiterpene derived from the rhizome of Zingiber zerumbet, which has attracted extensive attention in the recent decade for anticancer activities. The present study investigates the in vitro effect of zerumbone on rhabdomyosarcoma cells. Two rhabdomyosarcoma cell lines (RD and RH30) were used as the model system. The growth inhibition of zerumbone was measured by MTT-assay, apoptosis via flow cytometry, gene expression by real-time PCR, the migration by transwell assay, and intracellular signaling by Western blotting. Zerumbone shows anticancer effects on RD and RH30 cells in a dose-dependent manner via cell growth inhibition and induction of apoptosis. Exposure of RD and RH30 cells on zerumbone also resulted in a decrease of migration and downregulation of the hedgehog pathway. Taken together, our study provided the first evidence that zerumbone imparted strong inhibitory and apoptotic effects on pediatric rhabdomyosarcoma cell lines and merit further investigation as a promising candidate for the anticancer therapy.

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Journal of Cancer Research and Clinical Oncology

Tập 149

3313-3323

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