Antibacterial metal implant with a TiO2‐conferred photocatalytic bactericidal effect against Staphylococcus aureus

Surface and Interface Analysis - Tập 41 Số 1 - Trang 17-22 - 2009
Koutaro Shiraishi1, Hironobu Koseki1, Toshiyuki Tsurumoto1, K. Baba2, Mariko Naito3, Koji Nakayama3, Hiroyuki Shindo1
1Department of Orthopedic Surgery, Graduate School of Medicine, Nagasaki University, Japan
2Industrial Technology Center of Nagasaki, Japan
3Division of Microbiology and Oral Infection, Nagasaki University Graduate School of Biomedical Sciences, Japan

Tóm tắt

AbstractPhotocatalysis with anatase Titanium dioxide (TiO2) under ultraviolet A (UVA) has a well recognized bactericidal effect. There have been a few reports, however, on the effects of photocatalysis on bio‐implant‐related infections. The purpose of present study was to evaluate the photocatalytic bactericidal effects of anatase TiO2 on Staphylococcus aureus (S. aureus) associated with surgical site infections.TiO2 films were synthesized on commercially pure titanium substrates and SUS316 stainless steel using a plasma source ion implantation method followed by annealing. The chemical composition of the surface layers was determined using GXRD and XPS. The disks were seeded with cultured S. aureus and exposed to UVA illumination from black light. The bactericidal effect of the TiO2 films was evaluated by counting the survived colonies statistically.A structural gradient anatase type TiO2 layer formed on all substrates. The viability of the bacteria on the photocatalytic TiO2 film coated on titanium was suppressed to 7.0% at 30 minutes and 5.5% at 45 minutes, whereas that on a similarly coated stainless steel was suppressed to 45.8% at 30 minute and 28.6% at 45 minutes (ANOVA: p < 0.05). Complete bacterial inactivation was achieved after 90 minutes on titanium and after 60 minutes on stainless steel. The photocatalytic bactericidal effect of TiO2 is useful for sterilizing the contaminated surfaces of bioimplants. Copyright © 2008 John Wiley & Sons, Ltd.

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Surface and Interface Analysis

Tập 41 Số 1

17-22

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