Antibacterial activities of the extracts, fractions and compounds from Dioscorea bulbifera
Tóm tắt
Dioscorea bulbifera is an African medicinal plant used to treat microbial infections. In the present study, the methanol extract, fractions (DBB1 and DBB2) and six compounds isolated from the bulbils of D. bulbifera, namely bafoudiosbulbins A (1), B (2), C (3), F (4), G (5) and 2,7-dihydroxy-4-methoxyphenanthrene (6), were tested for their antimicrobial activities against Mycobacteria and Gram-negative bacteria involving multidrug resistant (MDR) phenotypes expressing active efflux pumps. The microplate alamar blue assay (MABA) and the broth microdilution methods were used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the above samples. The results of the MIC determinations indicated that when tested alone, the crude extract, fractions DBB1 and DBB2 as well as compounds 2 to 5 were able to prevent the growth of all the fifteen studied microorganisms, within the concentration range of 8 to 256 μg/mL. The lowest MIC value for the methanol extract and fractions (16 μg/mL) was obtained with DBB1 and DBB2 on E, coli AG100A and DBB2 on Mycobacterium tuberculosis MTCS2. The lowest value for individual compounds (8 μg/mL) was recorded with compound 3 on M. smegmatis and M. tuberculosis ATCC and MTCS2 strains respectively. The activity of the samples on many MDR bacteria such as Enterobacter aerogenes EA289, CM64, Klebsiella pneumoniae KP63 and Pseudomonas aeruginosa PA124 was better than that of chloramphenicol. When tested in the presence of the efflux pump inhibitor against MDR Gram-negative bacteria, the activity of most of the samples increased. MBC values not greater than 512 μg/mL were recorded on all studied microorganisms with fraction DBB2 and compounds 2 to 5. The overall results of the present investigation provided evidence that the crude extract D. bulbifera as well as some of the compounds and mostly compounds 3 could be considered as potential antimicrobial drugs to fight against MDR bacteria.
Tài liệu tham khảo
Rice LB: Unmet medical needs in antibacterial therapy. Biochem Pharmacol. 2006, 71: 991-995. 10.1016/j.bcp.2005.09.018.
World Health Organization: Tuberculosis, Fact sheet No. 104. 2010, World Health Organization, Geneva
Duke J: A, DuCellier JL: Handbook of Alternative Cash Crops. 1993, Boca Raton, CRC Press, Florida
Teponno RB, Tapondjou AL, Gatsing D, Djoukeng JD, Abou-Mansour E, Tabacchi R, Tane P, Stoekli-Evans H, Lontsi D, Park HJ: Bafoudiosbulbins A and B, two antisalmonellal clerodane diterpenoids from Dioscorea bulbifera var. sativa. Phytochemistry. 2006, 67: 1957-1963. 10.1016/j.phytochem.2006.06.019.
Teponno RB, Tapondjou AL, Jung HJ, Nam JH, Tane P, Park HJ: Three new clerodane diterpenoids from the bubils of Dioscorea bulbifera var. sativa. Helv Chim Acta. 2007, 90: 1599-1605. 10.1002/hlca.200790168.
Teponno RB, Tapondjou AL, Abou-Mansour E, Stoeckli-Evans H, Tane P, Barboni L: Bafoudiosbulbins F and G, further clerodane diterpenoids from Dioscorea bulbifera L. var sativa and revised structure of Bafoudiosbulbin B. Phytochemistry. 2008, 69: 2374-2379. 10.1016/j.phytochem.2008.06.008.
Kuete V, Ngameni B, Tangmouo JG, Bolla J-M, Alibert-Franco S, Ngadjui BT, Pagès J-M: Efflux pumps are involved in the Gram negative bacterial defense against isobavachalcone and diospyrone, two natural products. Antimicrob Agents Chemother. 2010, 54: 1749-1752. 10.1128/AAC.01533-09.
Eloff JN: A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med. 1998, 64: 711-713. 10.1055/s-2006-957563.
Mativandlela SPN, Lall N, Meyer JJM: Antibacterial, antifungal and antitubercular activity of (the roots of) Pelargonium reniforme (CURT) and Pelargonium sidoides (DC) (Geraniaceae) root. S Afr J Bot. 2006, 72: 232-237. 10.1016/j.sajb.2005.08.002.
Kuete V, Kamga J, Sandjo LP, Ngameni B, Poumale HM, Ambassa P, Ngadjui BT: Antimicrobial activities of the methanol extract, fractions and compounds from Ficus polita Vahl. (Moraceae). BMC Complement Altern Med. 2011, 11: 6-10.1186/1472-6882-11-6.
Kuete V, Ngameni B, Fotso Simo CC, Kengap Tankeu R, Tchaleu Ngadjui B, Meyer JJM, Lall N, Kuiate JR: Antimicrobial activity of the crude extracts and compounds from Ficus chlamydocarpa and Ficus cordata (Moraceae). J Ethnopharmacol. 2008, 120: 17-24. 10.1016/j.jep.2008.07.026.
Tereschuk ML, Riera MVQ, Castro GR, Abdala LR: Antimicrobial activity of flavonoid from leaves of Tagetes minuta. J Ethnopharmacol. 1997, 56: 227-232. 10.1016/S0378-8741(97)00038-X.
Zgoda JR, Porter JR: A convenient microdilution method screening natural products against bacteria and fungi. Pharmaceut Biol. 2001, 39: 221-225. 10.1076/phbi.39.3.221.5934.
Avila JG, de Liverant JG, Martínez A, Martínez G, Muñoz JL, Arciniegas A, de Vivar AR: Mode of action of Buddleja cordata verbascoside against Staphylococcus aureus. J Ethnopharmacol. 1999, 66: 75-78. 10.1016/S0378-8741(98)00203-7.
Jimenez-Arellanes A, Meckes M, Ramirez R, Torres J, Luna-Herrera J: Activity against multidrug-resistant Mycobacterium tuberculosis in Mexican plants used to treat respiratory diseases. Phytother Res. 2003, 17: 903-908. 10.1002/ptr.1377.
Teponno RB, Tapondjou AL, Djoukeng JD, Abou-Mansour E, Tabacchi R, Tane P, Lontsi D, Park HJ: Isolation and NMR assignment of a Pennogenin glycoside from Dioscorea bulbifera var sativa. Nat Prod Sci. 2006, 12: 62-66.
Simões M, Bennett RN, Rosa EA: Understanding antimicrobial activities of phytochemicals against multidrug resistant bacteria and biofilms. Nat Prod Rep. 2009, 26: 746-757. 10.1039/b821648g.
Kuete V: Potential of Cameroonian plants and derived products against microbial infections: a review. Planta Med. 2010, 76: 1479-1491. 10.1055/s-0030-1250027.
Kuete V, Efferth T: Cameroonian medicinal plants: pharmacology and derived natural products. Front Pharmacol. 2010, 1: 123-
Mims CA, Playfair JHL, Roitt IM, Wakelin D, Williams R: Antimicrobials and chemotherapy. Med Microbiol Rev. Edited by: Mims CA. 1993, 1-34. 35
Nicolle LE: Infection control programmes to contain antimicrobial resistance. WHO/CDS/CSR/DRS/2001.7. http://whqlibdoc.who.int/hq/2001/WHOCDSCSRDRS2001.7.pdf. Accessed January 2009
Chevalier J, Pagès J-M, Eyraud A, Malléa M: Membrane permeability modifications are involved in antibiotic resistance in Klebsiella pneumoniae. Biochem Biophys Res Commun. 2000, 274: 496-499. 10.1006/bbrc.2000.3159.
Savafi L, Duran N, Savafi N, Onlen Y, Ocak S: The prevalence and resistance patterns of Pseudomonas aeruginosa in intensive care units in a university hospital. Turk J Med Sci. 2005, 35: 317-322.
Zager EM, McNerney R: Multidrug-resistant tuberculosis. BMC Infect Dis. 2008, 8: 10-10.1186/1471-2334-8-10.
Corbett EL, Marston B, Churchyard GJ, De Cock KM: Tuberculosis in sub- Saharan Africa: opportunities, challenges, and change in the era of antiretroviral treatment. Lancet. 2006, 367: 926-927. 10.1016/S0140-6736(06)68383-9.
Newton SM, Lau C, Gurcha SS, Besra GS, Wright CW: The evaluation of forty-three plant species for in vitro antimycobacterial activities; isolation of active constituents from Psoralea corylifolia and Sanguinaria Canadensis. J Ethnopharmacol. 2002, 79: 57-67. 10.1016/S0378-8741(01)00350-6.
Shriram V, Jahagirdar S, Latha C, Kumar V, Puranik V, Rojatkar S, Dhakephalkar PK, Shitole MG: A potential plasmid-curing agent, 8-epidiosbulbin E acetate, from Dioscorea bulbifera L. against multidrug-resistant bacteria. Int J Antimicrob Agents. 2008, 32: 405-410. 10.1016/j.ijantimicag.2008.05.013.
Cowan MM: Plant products as antimicrobial agents. Clin Microbiol Rev. 1999, 12: 564-582.
The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/12/228/prepub