Antibacterial Effect of Kampo Herbal Formulation Hochu‐Ekki‐To (Bu‐Zhong‐Yi‐Qi‐Tang) on Helicobacter pylori Infection in Mice

Microbiology and Immunology - Tập 46 Số 7 - Trang 475-482 - 2002
Xiao-Qun Yan1, Masakazu Kita1, Masato Minami1, Toshiro Yamamoto1, Hiroko Kuriyama1, Tomoyuki Ohno2, Yoichiro Iwakura3, Jirô Imanishi1
1Department of Microbiology Kyoto Prefectural University of Medicine Kyoto Kyoto 602‐8566 Japan
23rd Department of Internal Medicine; Kyoto Prefectural University of Medicine; Kyoto Kyoto 602-8566 Japan
3Institute of Medical Science, Center for Experimental Medicine; University of Tokyo; Minato-ku Tokyo 108-8639 Japan

Tóm tắt

Abstract

Because Helicobacter pylori (H. pylori) infection is a major cause of gastroduodenal diseases in humans, the eradication of H. pylori using antibiotics is very effective for the treatment of gastroduodenal diseases. However, it has recently been reported that resistance to these antibiotics is developing. In the present study, the antibacterial effect of a Kampo (traditional Japanese medicine) herbal formulation, Hochu‐ekki‐to (HET; Formula repletionis animalis et supletionis medii), against H. pylori was examined in vitro and in vivo. HET inhibited the growth of antibiotic‐resistant strains of H. pylori as well as antibiotic‐sensitive strains at a dose of 2.5 mg/ml in vitro. When 1,000 mg/kg of HET was administered orally to C57BL/6 mice for 7 days before or after inoculation with H. pylori, H. pylori in the stomach was significantly reduced in the HET‐pre‐treatment group compared with the control group. Furthermore, HET in combination with antibiotics completely eradicated the bacteria in mice. The expression of interferon (IFN)‐γ was induced in the gastric mucosa of the mice pre‐treated with HET. There were no significant differences between the colonization of H. pylori in the control and HET treatment groups in IFN‐γ gene‐deficient mice. These results suggest that the antibacterial effect of HET may be partly due to IFN‐γ induction, and that HET may be clinically useful for treatment of H. pylori infection.

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Thông tin xuất bản

Microbiology and Immunology

Tập 46 Số 7

475-482

Thông tin tác giả