Anti-inflammatory, analgesic, antipyretic and antidiabetic activities of Abutilon hirtum (Lam.) Sweet
Tóm tắt
Many plants of genus Abutilon are traditionally used for treatment of inflammation, bronchitis, piles, gonorrhea, diabetes and fever. Abutilon hirtum is traditionally used to ease the pain of kidney gravel, to treat diarrhoea, cough and toothache, to cure bladder inflammations, wounds and ulcers and as an antipyretic, demulcent, diuretic and mouth wash. The aim of the study is to evaluate the anti-inflammatory, analgesic, antipyretic and antidiabetic effects of the total ethanolic extract and different fractions of Abutilon hirtum (Lam.) Sweet leaves. Air dried powder of A. hirtum leaves were extracted using 95% ethanol and fractionated successively with petroleum ether, chloroform and finally with ethyl acetate. The extracts were concentrated to afford petroleum ether, chloroform, ethyl acetate and aqueous fractions and were investigated for their anti-inflammatory, analgesic, antipyretic and antidiabetic activities using carrageenan-induced paw edema, hot plate, yeast -induced pyrexia and streptozotocin-induced hyperglycemia methods, respectively. The total ethanolic extract and the chloroform fraction exhibited the highest anti-inflammatory activity with a percentage of inhibition 50.8% which is close to that of indomethacin (52.4%). The aqueous extract exhibited the maximum analgesic activity (216.6%) with a rapid onset and a longer duration followed by petroleum ether and chloroform fractions and total extract (189.8, 186.9 and 183.0%, respectively), which is almost similar to that of acetylsalicylic acid (186.4%). The total ethanolic extract showed higher activity compared to the used standard acetylsalicylic acid with a rapid onset (30 min) and a longer duration exhibiting the maximum activity. The crude polysaccharides fraction showed a significant lowering in blood glucose level (81.08%). The total extract and petroleum ether, chloroform, ethyl acetate and aqueous fractions exhibited a significant anti-diabetic activity after 5 h (47.49, 47.79, 50.04, 49.80 and 46.36%, respectively) compared with that of metformin (55.45%). Abutilon hirtum extract and fractions exhibited anti-inflammatory analgesic, antipyretic and antipyretic activities which may be attributed by the presence of active phytoconstituents.
Tài liệu tham khảo
Arbat AA. Pharmacognostic studies of stem of Abutilon pannosum (Forst F.). Biosci Discov. 2012;3:317–20.
Brink M, Dako EG. Plant Resources of Tropical Africa 16. Fibres. Wageningen, Netherlands: Prota Foundation; 2012. p. 25.
Khadabadi SS, Bhajipale NS. A review on some important medicinal plants of Abutilon spp. Res J Pharm Biol. Chem Sci. 2010;1:718–29.
Wesley SP, Devi CB, Moin S, Shibu SB. In vitro phytochemical screening, free radical scavenging activity and anticancer activity of Abutilon hirtum (Lam.) Sweet (Malvaceae). Int J PharmTech Res. 2013;5:155–61.
Gomaa AA, Samy MN, Desoukey SY, Kamel MS. Pharmacognostical studies of leaf, stem, root and flower of Abutilon hirtum (Lam.) Sweet. Int J Pharmacogn Phytochem Res. 2016;8:199–216.
Srinivas RK, Sanjeeva KA, Gnananath K, Ganapaty S. Hepatoprotective potential of Abutilon hirtum Sweet leaves in carbon tetrachloride induced hepatotoxicity. Asian J Biomed Pharm Sci. 2011;1:26–31.
Kassem HA. Investigation of lipids, mucilage and cytotoxic activity of Abutilon hirtum (Lam.) Sweet grown in Egypt. Bull Fac Pharm. Cairo Univ. 2001;39:156–9.
Saraswathi R, Upadhyay L, Venkatakrishnan R, Meera R, Devi P. Phytochemical investigation, analgesic and anti- inflammatory activity of Abutilon indicum linn. Int J Pharm Pharm Sci. 2011;3:154–6.
Sharma A, Sharma RA, Singh H. Phytochemical and Pharmacological Profile of Abutilon indicum L. Sweet: A Review. Int J Pharm Sci Rev Res. 2013;20:120–7.
Kaladhar DK, Saranya SK, Vadlapudi V, Yarla NS. Evaluation of anti-inflammatory and anti-proliferative activity of Abutilon indicum L. plant ethanolic leaf extract on lung cancer cell line A 549 for system network studies. Cancer Sci Ther. 2014;6:195–201.
Kushwaha SK, Dashora A, Patel JR, Kori ML. Antinociceptive and anti-inflammatory activities of quercetin isolated from ethanolic extract of Abutilon indicum L. Novus Nat Sci Res. 2014;3:8–15.
Akapa TC, Kehinde AO, Beatrice OO, Olajide OJ. Antipyretic activity of Abutilon mauritianum (Jacq.) roots in Wistar rats. Int J Pharm Sci Res. 2014;5:42–6.
Kassem HA. Study of polyphenolic components and macro- and micromorphological characters of Abutilon hirtum (Lam.) Sweet. Bull Fac Pharm. Cairo Univ. 2007;45:173–83.
Akhila JS, Deepa S, Alwar MC. Acute toxicity studies and determination of median lethal dose. Current Sci. 2007;93:917–20.
Winter CA, Risley GA, Nuss GW. Carrageenan induced edema in hind paw of the rat as an assay for inflammatory drugs. Proc Soc Exp Biol Med. 1962;111:544–7.
Sudjarwo AS. The Potency of piperine as anti-inflammatory and analgesic in rats and mice. Folia Medica Indonesiana. 2005;41:190–4.
Hosseinzadeh H, Ramezani M, Salmani G. Antinociceptive, anti-inflammatory and acute toxicity effects of Zataria multiflora Boiss extracts in mice and rats. J Ethnopharmcol. 2000;73:379–85.
Magaji M, Anuka J, Abdu-Aguye I, Yaro A, Hussaini I. Preliminary studies on anti-inflammatory and analgesic activities of Securinega virosa (Euphorbiaceae) in experimental animal models. J Med Plants Res. 2008;2:39–44.
Adams SS, Hebborn P, Nicholson JS. Some aspects of the pharmacology of ibufenac, a non-steroidal anti-inflammatory agent. J Pharm Pharmacol. 1968;20:305–12.
Panthong A, Kanjanapoth D, Taesotikul T, Wongcome T, Reutrakul V. Anti-inflammatory and antipyretic properties of Clerodendrum petasites S. Moore J Ethanopharmacol. 2003;85:151–6.
Gruber HE, Schedl HP, Osborne JW. Alterations in islet cell ultrastructure following streptozotocin-induced diabetes in the rat. ActaDiabetologia Latina. 1980;17:213–23.
Peungvicha P, Thirawarapan SS, Temsiririrkkul R, Watanabe H, Prasain JK, Kadota S. Hypoglycemic effect of the water extract of Piper sarmentosum in rats. J Ethnopharmcol. 1998;60:27–32.
Tanko Y, Okasha MA, Saleh IA, Mohammed A, Yerima M, Yaro AH, Isa AI. Anti-diabetic effect of ethanolic flower extracts of Newbouldia laevis (Bignoniaceae) on blood glucose levels of streptozocin-induced diabetic wistar rats. Res J Med Sci. 2008;2:62–5.
Oany AR, Al Siddikey A, Hossain MU, Islam R, Emran A-A. A preliminary evaluation of cytotoxicity, antihyperglycemic and antinociceptive activity of Polygonum hydropiper L. ethanolic leaf extract. Clinical Phytoscience. 2016;2:2.
Prasad PJ. Conceptual Pharmacology. India: University Press Private limited; 2010. p. 55.
Owoyele BV, Oguntoye SO, Dare K, Ogunbiyi BA, Aruboula EA, Soladoye AO. Analgesic, anti-inflammatory and antipyretic activities from flavonoid fractions of Chromolaena odorata. J Med Plants Res. 2008;2:219–25.
Niazi J, Gupta V, Chakarborty P, Kumar P. Anti-inflammatory and antipyretic activity of aleuritis moluccana leaves. Asian J Pharm Clinical Res. 2010;3:35–7.
Nijveldt RJ, Nood EV, Hoorn DE, Boelens PG, Norren KV, Paul AL. Flavonoids: a review of probable mechanisms of action and potential applications. Amer J Clinical Nutri. 2001;74:418–25.
Krisanapun C, Lee S, Peungvicha P, Temsiririrkkul R, Baek SJ. Antidiabetic activities of Abutilon indicum (L.) Sweet are mediated by enhancement of adipocyte differentiation and activation of the GLUT1 promoter. Evidence-Based Complement Alternat Med. 2011;2011:1–9.
Chen X, Jin J, Tang J, Wang Z, Wang J, Jin L, Lu J. Extraction, purification, characterization and hypoglycemic activity of a polysaccharide isolated from the root of Ophiopogon japonicas. Carbohydr Polymers. 2011;83:749–54.
Li SP, Zhang GH, Zeng Q, Huang ZG, Wang YT, Dong TT, Tsim KW. Hypoglycemic activity of polysaccharide, with antioxidation, isolated from cultured Cordyceps mycelia. Phytomedicine. 2006;13:428–33.