Anti-diabetic activities of Chromolaena odorata methanol root extract and its attenuation effect on diabetic induced hepatorenal impairments in rats
Tóm tắt
Chromolaena odorata is a medicinal plant whose root has not been reported for detailed anti-diabetic properties. Hence, this study investigated the anti-diabetic properties of the methanol root extract of Chromolaena odorata and its effect on biochemical parameters in alloxan induced diabetic rats. In-vitro studies were carried out using α-amylase inhibition, glycosylated heamoglobin inhibition and glucose uptake test in yeast cells. Twenty (20) alloxan (120 mg/kg bw) induced diabetic rats were divided into 4 groups and treated with 0, 300 and 600 mg/kg bw of the extract and 5 mg/kg b.wt glibenclamide respectively. All treatments were administered daily for 14 days through oral route with the aid of esophageal cannula. Five (5) rats were also set up as normal control. Serum biochemical parameters were analysed. Chromolaena odorata exhibited strong inhibition of α-amylase activity and glycosylated heamoglobin with IC 50 values; 533.05 μg/ml and 679.12 μg/ml respectively Extract doses of 300 and 600 mg/kg bw exhibited 49.86% and 68.30% in vivo hypoglycemic effect and increase the weight gain of animals to 13.23 ± 0.67 g and 13.87 ± 0.67 g respectively. The concentrations of sodium, chloride, bicarbonates, aspartate transaminase (AST), alkaline phosphatase (ALP) and total proteins were significantly (p < 0.05) elevated while albumin, direct and total bilirubins were lowered in diabetic untreated rats when compared with the control Treatment with extract at 300 and 600 mg/kg bw significantly (p < 0.05) restored the concentrations of AST, ALP, albumin, total proteins, direct and total bilirubins towards their normal levels but could not significantly (P > 0.05) attenuate the elevated sodium, chloride, bicarbonates, urea and creatinine concentration when compared with the untreated control. Chromolaena odorata root extract exhibited anti-diabetic and protective effect against diabetic induced hepatic impairment. However, diabetic induced renal impairment was not attenuated by treatment with Chromolaena odorata in rats.
Tài liệu tham khảo
Belayneh YM, Birru EM. Anti-diabetic Activities of Hydromethanolic Leaf Extract of Calpurnia aurea (Ait.) Benth. Subspecies aurea (Fabaceae) in Mice. Hindawi Evidence-Based Complementary and Alternative Medicine, 2018, Article ID 3509073, 9 pages.
World Health Organisation. Global estimates of the prevalence of diabetes for 2017. Geneva: Diabetes WHO fact sheet; 2018.
IDF. Diabetes, “Atlas,” Brussels, Belgium, 2015.
Whiting DR, Guariguata L, Weil C, Shaw J. IDF Diabetes atlas: global estimates of the prevalence of diabetes for 2011 and 2030. Diabetes Res Clin Pract. 2011;94(3):311–21.
Lawal B, Shittu OK, Inje OF, Berinyuy EB, Muhammed H. Potential antioxidants and Hepatoprotectives from African natural products: a review. Clin Phytosci. 2017;2(23):1–66. https://doi.org/10.1186/s40816-016-0037-0.
Bashir L, Shittu OK, Sani S, Busari MB, Adeniyi KA. African natural products with potential Antitrypanosoma properties: a review. Int J Bioch Res Rev. 2015;7(2):45–79.
Lawal B, Shittu OK, Kabiru AY, Jigam AA, Umar MB, Berinyuy EB, Alozieuwa BU. Potential antimalarials from African natural products: a review. J Intercult Ethnopharmacol. 2015;4(4):318–43. https://doi.org/10.5455/jice.20150928102856.
Upendra M, Rao M, Sreenivasulu B, Chengaiah K, Jaganmohan R, Madhusudhana C. Herbal medicines for diabetes mellitus: a review. Inter J PharmTech Res. 2010;2(3):1883–92.
Bamisaye F, Ajani E, Nurain O, Minari J. Medico-botanical investigation of siam weed (chromolaena odorata) used among the “ijebu” people of Ogun state, Nigeria. J Med Med Sci. 2014;5(1):20–4.
Akinmoladun A, Akinloye O. Effect of Chromolaena odorata on hypercholesterolemia-related metabolic imbalances. In: 3rd SAAT Annual Conference. Nigeria: FUTA; 2007. p. 287–90.
Phan T, Wang L, See P, Grayer J, Chan Y, Lee T. Phenolic compounds of Chromolaena odorata protect cultured skin cells from oxidative damage: implication for cutaneous wound healing. Biol Pharm Bull. 2001;24(12):1373–9.
Zachariades C, Day M, Muniappan R, Reddy GVP. Chromolaena odorata (L.) king and Robinson (Asteraceae). In: Biological control of tropical weeds using arthropods. Cambridge: Cambridge University Press; 2009. p. 130–62.
Chakraborty AK, Rambhade S, Patil UK. Chromolaena odorata (L.): an overview. J Pharm Res. 2011;4(3):573–6.
Naidoo KK, Coopoosamy RM, Naidoo G. Screening of Chromolaena odorata L king and Robinson for antibacterial and antifungal properties. J Med Plant Res. 2011;519:4839–62.
Robert KO, Nwanebu FC, Uduak U, Nna JN, Lydia NO, Nnaemeka C. Ethanolic extraction and phytochemical screening of two Nigeria HerbsChromolaena odorata and Citrus sinensis on pathogens isolated from wound infections. Int J Compr Pharm. 2011;210:1–9.
Nickavar B, Yousefian N. Inhibitory effects of six Allium species on α-amylase enzyme activity. Iran J Pharmacol Res. 2009;8:53–7.
Hansawasdi C, Kawabata J, Kasai T. α- amylase inhibitors from Roselle (Hibiscus sabdariffa Linn.) tea. Biosci Biotechnol Biochem. 2000;64:1041–3.
Bashir L, Shittu OK, Busari MB, Sani S, Aisha MI. Safety evaluation of giant African land snails (Archachatina maginata) haemolymph on hematological and biochemical parameters of albino rats. J Adv Med Pharm Sci. 2015;3(3):122–30.
Asgary S, Naderi GH, Sarrafzadegan N, Ghassemi N, Boshtam M, Rafie M, Arefian A. Anti-oxidant effect of flavonoids on hemoglobin glycosylation. Pharm Acta Helv. 1999;73(5):223–6.
Abirami N, Natarajan A, Sagadevan B. Phytochemical investigation and in vitro evaluation of hypoglycemic potential of grewia hirsute. Int J Pharm Bio Sci. 2014;5(1):76–83.
Mary S, Gayathri D. In vitro glucose binding activity of Terminalia bellirica. Asian J Pharm Clin Res. 2015;8(2):320–3.
Amos TN, Bashir L, Saba SE, Saba MA, Mohammed BM, Abdulsalam IH, et al. Phytochemicals and acute toxicity profile of aqueous and methanolic extracts of Crateva adansonii leaves in Swiss albino rats. Asian J Biochem. 2015;10(4):173–9.
Etuk EU. Animals models for studying diabetes mellitus. Agric Biol J. 2010;1(2):130–4.
Lawal B, Shittu OK, Abubakar AN, Umar MB, Ibrahim AM, Haruna GM. Biochemical evaluation in Wister rats (Rattus novergicus) following chronic exposure of methanol leaf extract of Telfairia occcidentalis. J Pharm Biomed Sci. 2015;5(9):740–4.
Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol. 1957;28:56–63.
Wright PJ, Plummer DT. The use of urinary enzyme measurement to detect renal damages caused by nephrotoxic compounds. Biochem Pharmacol. 1974;12:65–73.
Gornall AC, Bardawill CJ, David MM. Determination of serum protein by means of biuret reaction. J Biol Chem. 1949;177:751–66.
Doumas BT, Watson WA, Biggs HG. Albumin standards and the measurement of serum album with bromocresol green. Clin Chem Acta. 1971;31:87–96.
Tietz NW. Clinical guide to laboratory tests. 3rd ed. PhiladelphiaPA: WB Saunders Company; 1995. p. 286–8.
Burtis CA, Ashwood ER, Bruns DE. TIETZ Textbook of Clinical Chemistry and Molecular diagnostics. 4th edition. Elsevir; 2006. p. 702–8.
Heinegard D, Tinderstrom G. Determination of serum creatinine by a direct colorimetric method. Inter J Clin Chem. 1973;43:395–410.
SAS. Statistical analysis system, SAS users guide: statistics. Cary: SAS institute Inc; 2008.
Sarma A, Mallick A, Ghosh A. Free radicals and their role in different clinical conditions: an over- view. Int J Pharm Sci Res. 2010;1:185–92.
Oboh G, Ademiluyi A, Akinyemi A, Saliu J. Schwarzenbolz u. inhibitory effect of polyphenol-rich extracts of jute leaf (Corchorus olitorius) on key enzyme linked to type-2 diabetes (α- amylase and α-glucosidase) and hypertension (angiotensin I converting) in vitro. J Funct Foods. 2012;4(2):450–8.
Buziol S, Becker J, Baumeister A. Determination of in vivo kinetics of the starvation-induced Hxt5 glucose transporter of Saccharomyces cerevisiae. FEMS Yeast Res. 2002;2:283–91.
Ogbonna O, Mbaka G, Anyika E, Osegbo O, Igbokwe H. Evaluation of acute toxicity in mice and subchronic toxicity of hydro-ethanolic extract of Chromolaena odorata in rats. Agric Biol J N Am. 2010;1(5):859.
Chang-Yong Y, Jing W, Yuan Z, Xing J, Zhen-Guo X. Anti-diabetic effects of Panax notoginseng saponins and its major anti-hyperglycemic components. J Ethnopharmacol. 2010;130:231–6.
Kumari M, Jain S, Dave R. Babul (Acacia nilotica), A. potential source of tannin and its suitability in management of type II diabetes. Nutri Food Sci. 2014;44:119–26.
Ijioma SN, Okafor AI, Ndukuba PI, Nwankwo AA, Akomas SC. Hypoglycemic, hematologic and lipid profile effects of Chromolaena odorata ethanol leaf extract in alloxan induced diabetic rats. Ann Biol Sci. 2014;2:27–32.
Shittu OK, Lawal B, Abubakar NA, Berinyuy BE, Busari MB, Ibrahim AO. Toxicological implications of methanol extract from Nigerian bee propolis on some selected rat tissues. J Pharm Biomed Sci. 2015;5(7):524–31.
Shittu OK, Lawal B, Adeniyi AK, Kilani LT, Saka RB. Effect of methanol extract of Musca domestica larva on some enzymes and Haematological parameters in Trypanosoma brucei brucei - infected rats. Nig J Basic Appl Sci. 2017;25(2):66–74. https://doi.org/10.4314/njbas.v25i2.8.
Shittu OK, Lawal B, Haruna GM, Berinyuy EB, Yusuf AA, Ibrahim AM. Hepato-curative effects of methanol extract from Nigerian bee propolis in carbon tetrachloride (CCl4) intoxicated rat. Eur J Biotechnol Biosci. 2015;3(7):1–4.
Yusuf AA, Lawal B, Yusuf MA, Omonije YO, Adejoke AA, Raji FH, Wenawo DL. Free radical scavenging, antimicrobial activities and effect of sub-acute exposure to Nigerian Xylopia Aethiopica seed extract on liver and kidney functional indices of albino rat. Iran J Toxicol. 2018;12(3):51–8.
Lawal B, Shittu OK, Ossai PC, Abubakar AN, Ibrahim AM. Antioxidant activities of Giant African snail (Achachatina maginata) Haemolymph against CCl4- induced hepatotoxicity in albino rats. Br J Pharm Res. 2015;6(3):141–54.
Lawal B, Shittu OK, Oibiokpa IF, Mohammed H, Umar SI, Haruna GM. Antimicrobial evaluation, acute and sub-acute toxicity studies of Allium sativum. J Acute Dis. 2016;5(4):296–301.
Malawad B, Usha A. Plasma proteins in type 2 Diabetes mellitus. J Biotechnol Biochem. 2011;2(5):01–3.
Ladei A, Stefanelli E, Ladeia-frota C, Moreira A, Adan L. Association between elevatednserum C-reactive protein and triglyceride levels in young subjects with type-1 diabetes. Diabetes Care. 2006;29:424–6.
Shittu OK, Lawal B, Alozieuwa BU, Haruna GM, Abubakar AN, Berinyuy EB. Alteration in biochemical indices follopapatawing chronic administration of methanolic extract of Nigeria bee propolis in Wister rats. Asian Pac J Trop Dis. 2015;5(8):654–7.
Hemangi S, Bhonlet H. Low plasma albumin levels are associated with increased plasma protein glycation and Hba1c in Diabetes. J Proteome Res. 2012;11(2):1391–6.
Libor V. The role of bilirubin in Diabetes, metabolic syndrome, and cardiovascular diseases. Front Pharmacol. 2012;3:55–61.
Lin P, Vítek L, Schwertner A. Serum bilirubin and genes controlling bilirubin concentrations as biomarkers for cardiovascular disease. Clin Chem. 2010;56:1535–43.
Zilva JF, Panmall PR, Mayne PD. Clinical chemistry in diagnosis and treatment. 5th ed. England: England Clays Ltd. St. Ives Plc; 1991.
Aldler I, Stevens J, Manley E, Bilous R, Cull A, Holman R. Development and progression of nephropathy in type 2 diabetes: the United Kingdom prospective Diabetes study (UKPDS 64). Kidney Int. 2003;63:225–32.
Judykay T. Nutrition for reducing urea and creatinine in the blood. Diabetes Care. 2017;27:2191–2.