Anthraquinone in Indonesian infusion tea: analysis by HPLC–UV and risk assessment
Tóm tắt
Detection of anthraquinone in tea samples marketed in Europe is raising a concern due to the possible carcinogenicity of this compound. The European Union has set a very low maximum residue level (MRL) for anthraquinone residue in tea (0.02 mg/kg). The available method analyses for anthraquinone are mostly by gas chromatography (GC) coupled by mass spectrophotometer (MS) which are relatively more expensive instruments and not always available to moderately equipped laboratories. This study was aimed to analyze anthraquinone in infusion tea of Indonesian commercial tea using liquid chromatography–ultraviolet detector (HPLC–UV) and then assessed the risk associated with consuming infusion tea with anthraquinone residue. The analysis was conducted by low-volume liquid–liquid extraction followed by quantification by HPLC–UV, while the risk assessment was analyzed by calculating the risk quotient (HQ) and carcinogenic risk (R) based on the highest anthraquinone level detected in the sample. The low-volume liquid–liquid extraction followed by HPLC quantification was able to analyze anthraquinone in infusion tea with linearity of 0.9990 at 8.33 to 83.33 μg/L; precision of 2.25%; and recovery of 96.19 to 102.98%. The anthraquinone levels in infusion tea of Indonesian commercial tea varied from not detected to 0.44 μg/L. The calculated HQ is lower than 1 (0.033), implying that consuming Indonesian infusion tea with the detected anthraquinone residue is unlikely to cause non-carcinogenic effect. The R is 2.63 × 10−6, but no parametric values have been regulated for anthraquinone; thus, its carcinogenic effect cannot be further assessed. Low-volume liquid–liquid extraction followed by quantification by HPLC–UV was able to analyze anthraquinone in infusion tea of Indonesian commercial tea samples. Risk assessment showed no prove of adverse effect related to consuming infusion tea as high as the highest concentration detected in infusion tea samples. However, risk associated at higher concentration and/or long-term consumption may not be neglected.
Tài liệu tham khảo
BNN. Public statement on the application of the BNN orientation value for biphenyl and anthraquinone detected in organic herbs, spices, herbal teas and tea (Camellia sinensis). Berlin: Bundesverband Naturkost Naturwaren; 2015.
Wang X, Zhou L, Luo F, Zhang X, Sun H, Yang M, Lou Z, Chen Z. 9, 10-Anthraquinone deposit in tea plantation might be one of the reasons for contamination in tea. Food Chem. 2018;244:254–9. https://doi.org/10.1016/j.foodchem.2017.09.123.
EU. COMMISSION REGULATION (EU) No 1146/2014. Official Journal of the European Union; 2014.
NTP. NTP Toxicology and Carcinogenesis Studies of ANTHRAQUINONE (CAS NO. 84-65–1) Feed Studies in F344/N Rats and B6C3F1 Mice. National Toxicology Program technical report series. 2005;494:1.
IARC. IARC Monographs on the evaluation of carcinogenic risks to humans. Volume 101. 2013; 2013.
Hazardous Substance Data Bank. Anthraquinone. U.S. National Library of Medicine. 2006.
Thomson R. Naturally occurring quinones. Amsterdam: Elsevier; 2012.
DeLiberto ST, Werner SJ. Review of anthraquinone applications for pest management and agricultural crop protection. Pest Manag Sci. 2016;72(10):1813–25. https://doi.org/10.1002/ps.4330.
Yu M-L, Hites RA. Identification of organic compounds on diesel engine soot. Anal Chem. 1981;53(7):951–4. https://doi.org/10.1021/ac00230a005.
Galceran MT, Moyano E. Determination of oxygenated and nitro-substituted polycyclic aromatic hydrocarbons by HPLC and electrochemical detection. Talanta. 1993;40(5):615–21. https://doi.org/10.1016/0039-9140(93)80266-T.
Oda J, Maeda I, Mori T, Yasuhara A, Saito Y. The relative proportions of polycyclic aromatic hydrocarbons and oxygenated derivatives in accumulated organic particulates as affected by air pollution sources. Environ Technol. 1998;19(10):961–76. https://doi.org/10.1080/09593331908616755.
Sklorz M, Briedé J-J, Schnelle-Kreis J, Liu Y, Cyrys J, de Kok TM, et al. Concentration of oxygenated polycyclic aromatic hydrocarbons and oxygen free radical formation from urban particulate matter. J Toxicol Environ Health A. 2007;70(21):1866–9. https://doi.org/10.1080/15287390701457654.
James RH, Adams RE, Finkel JM, Miller HC, Johnson LD. Evaluation of analytical methods for the determination of POHC in combustion products. J Air Pollut Control Assoc. 1985;35(9):959–69. https://doi.org/10.1080/00022470.1985.10465986.
Akimoto Y, Aoki T, Nito S, Inouye Y. Oxygenated polycyclic aromatic hydrocarbons from MSW incinerator fly ash. Chemosphere. 1997;34(2):263–73. https://doi.org/10.1016/S0045-6535(96)00376-1.
EURL. EURL-PROFICIENCY TEST-T02, 2014 pesticide residues in tea homogenate 2014.
Barrera Vázquez MF, Comini LR, Martini RE, Núñez Montoya SC, Bottini S, Cabrera JL. Comparisons between conventional, ultrasound-assisted and microwave-assisted methods for extraction of anthraquinones from Heterophyllaea pustulata Hook f. (Rubiaceae). Ultrason Sonochem. 2014;21(2):478–84. https://doi.org/10.1016/j.ultsonch.2013.08.023.
Duval J, Pecher V, Poujol M, Lesellier E. Research advances for the extraction, analysis and uses of anthraquinones: a review. Ind Crops Prod. 2016;94:812–33. https://doi.org/10.1016/j.indcrop.2016.09.056.
Deng S, West BJ, Jensen CJ, Basar S, Westendorf J. Development and validation of an RP-HPLC method for the analysis of anthraquinones in noni fruits and leaves. Food Chem. 2009;116(2):505–8. https://doi.org/10.1016/j.foodchem.2009.02.070.
Hayward DG, Wong JW, Park HY. Determinations for pesticides on Black, Green, Oolong, and White Teas by gas chromatography triple-quadrupole mass spectrometry. J Agric Food Chem. 2015;63(37):8116–24. https://doi.org/10.1021/acs.jafc.5b02860.
Rahman MM, Abd El-Aty A, Kim SW, Shin SC, Shin HC, Shim JH. Quick, easy, cheap, effective, rugged, and safe sample preparation approach for pesticide residue analysis using traditional detectors in chromatography: a review. J Sep Sci. 2017;40(1):203–12. https://doi.org/10.1002/jssc.201600889.
Shi W, Zhang F, Zhang X, Su G, Wei S, Liu H, et al. Identification of trace organic pollutants in freshwater sources in Eastern China and estimation of their associated human health risks. Ecotoxicology. 2011;20(5):1099–106. https://doi.org/10.1007/s10646-011-0671-8.
Hu Y, Qi S, Zhang J, Tan L, Zhang J, Wang Y, et al. Assessment of organochlorine pesticides contamination in underground rivers in Chongqing, Southwest China. J Geochem Explor. 2011;111(1–2):47–55. https://doi.org/10.1016/j.gexplo.2011.07.006.
USEPA. Supplementary guidance for conducting health risk assessment of chemical mixtures. Risk Assessment Forum Technical Panel. Office EPA/360/R-00/002.: US Environmental Protection Agency. 2000.
Kim HH, Lim YW, Yang JY, Shin DC, Ham HS, Choi BS, Lee JY. Health risk assessment of exposure to chlorpyrifos and dichlorvos in children at childcare facilities. Sci Total Environ. 2013;444:441–50. https://doi.org/10.1016/j.scitotenv.2012.11.102.
USEPA. provisional peer-reviewed toxicity values for 9,10-Anthraquinone (CASRN 84-65-1), Superfund Health Risk Technical Support Center, National Center for Environmental Assessment Office of Research and Development. U.S. Environmental Protection Agency. 2011.
PPID. Outlook Teh Komoditas Pertanian Subsektor Perkebunan: Pusat Data dan Sistem Informasi Pertanian, Sekretariat Jenderal Kementerian Pertanian. 2016.
Fan CL, Chang QY, Pang GF, Li ZY, Kang J, Pan GQ, Zheng SZ, Wang WW, Yao CC, Ji XX. High-throughput analytical techniques for determination of residues of 653 multiclass pesticides and chemical pollutants in tea—part V: a comparative study of the influence of tea hydration on the efficiency of pesticide multiresidue determination using three sample preparation methods and GC/MS/MS. J AOAC Int. 2015;98(1):149–59. https://doi.org/10.5740/jaoacint.13-279.
Wang F, Li S, Feng H, Yang Y, Xiao B, Chen D. An enhanced sensitivity and cleanup strategy for the nontargeted screening and targeted determination of pesticides in tea using modified dispersive solid-phase extraction and cold-induced acetonitrile aqueous two-phase systems coupled with liquid chromatography–high resolution mass spectrometry. Food Chem. 2019;275:530–8. https://doi.org/10.1016/j.foodchem.2018.09.142.
Wang J, Cheung W, Leung D. Determination of pesticide residue transfer rates (percent) from dried tea leaves to brewed tea. J Agric Food Chem. 2014;62(4):966–83. https://doi.org/10.1021/jf404123h.
AOAC. Appendix F: guidelines for Standard METHOD performance requirements, Official Methods of Analysis of AOAC 2016.
EC. Guidance document on pesticide residue analytical methods 2010. https://ec.europa.eu/food/sites/food/files/plant/docs/pesticides_ppp_app-proc_guide_res_post-reg-cont-monitor.pdf.
BPS-RI. Pengeluaran untuk Konsumsi Penduduk Indonesia, Maret 2018: Badan Pusat Statistik Republik Indonesia. 2018.