Anion homeostasis is important for non-lytic release of BK polyomavirus from infected cells

Open Biology - Tập 5 Số 8 - Trang 150041 - 2015
Gareth L. Evans1, Laura Caller2, Victoria Foster3, Colin M. Crump4
1Gareth L. Evans Google Scholar Find this author on PubMed
2Laura G. Caller Google Scholar Find this author on PubMed
3Victoria Foster Google Scholar Find this author on PubMed
4Colin M. Crump [email protected] Google Scholar Find this author on PubMed

Tóm tắt

BK polyomavirus (BKPyV) is a member of a family of potentially oncogenic viruses, whose reactivation can cause severe pathological conditions in transplant patients, leading to graft rejection. As with many non-enveloped viruses, it is assumed that virus release occurs through lysis of the host cell. We now show the first evidence for a non-lytic release pathway for BKPyV and that this pathway can be blocked by the anion channel inhibitor DIDS. Our data show a dose-dependent effect of DIDS on the release of BKPyV virions. We also observed an accumulation of viral capsids in large LAMP-1-positive acidic organelles within the cytoplasm of cells upon DIDS treatment, suggesting potential late endosome or lysosome-related compartments are involved in non-lytic BKPyV release. These data highlight a novel mechanism by which polyomaviruses can be released from infected cells in an active and non-lytic manner, and that anion homeostasis regulation is important in this pathway.

Từ khóa


Tài liệu tham khảo

10.1016/S0140-6736(71)91776-4

10.1016/S0140-6736(71)91777-6

10.1126/science.1152586

10.1038/jid.2008.302

10.1007/s00705-011-1008-x

10.1038/nrmicro2992

10.1016/j.micinf.2012.02.002

10.1093/jnci/djp332

10.1371/journal.ppat.1000363

10.1155/2013/926391

10.1086/431522

10.1093/infdis/147.4.676

10.1007/0-387-32957-9_11

10.1056/NEJM198607243150405

10.1038/sj.bmt.1702487

10.1097/00007890-199901150-00018

10.1111/j.1399-3062.2006.00150.x

10.1016/j.semcancer.2009.02.004

10.1016/0092-8674(79)90209-5

10.1099/vir.0.009159-0

10.3109/13550289509113966

10.1016/j.virol.2012.12.015

10.1128/JVI.01819-08

10.1371/journal.ppat.1000126

10.1038/nature12029

Clayson ET, 1989, Release of Simian Virus-40 virions from epithelial-cells is polarized and occurs without cell-lysis, J. Virol, 63, 2278, 10.1128/JVI.63.5.2278-2288.1989

10.1152/physrev.00029.2001

10.1038/nrd2780

10.1152/ajpcell.00299.2005

10.1016/0005-2736(89)90427-6

10.1038/sj.embor.7400169

IUPHAR/BPS. Guide to pharmacology. Accessed 28 January 2015. See http://www.guidetopharmacology.org/GRAC/LigandDisplayForward?tab=biology&ligandId=4177.

10.1074/jbc.274.2.896

10.1074/jbc.M002712200

10.1038/ncb1773

10.1113/jphysiol.1996.sp021396

10.1016/S0006-8993(00)02350-7

10.1113/jphysiol.1994.sp020049

10.1097/00001756-199510010-00021

10.1371/journal.pone.0043995

10.1016/j.virol.2004.03.027

10.1128/JVI.02169-08

Sigma-Aldrich. 2014 4 4′-Diisothiocyanatostilbene-2 2′-disulfonic acid disodium salt hydrate. See http://www.sigmaaldrich.com/catalog/product/fluka/38440?lang=en&region=GB.

10.1371/journal.ppat.1002630

10.1101/cshperspect.a016840

10.1073/pnas.77.5.2721

10.1113/jphysiol.2006.124719

10.1146/annurev.physiol.67.032003.153245

10.1038/nature03720

10.1371/journal.pone.0000474

10.1038/nature06907

10.1073/pnas.95.14.7843

10.1016/j.bbrc.2005.02.060

10.1007/s00424-002-0950-6

10.1038/sj.emboj.7600576

10.1073/pnas.0606137103

10.1128/JVI.01571-06

10.1099/vir.0.035444-0

Thông tin
Thông tin xuất bản

Open Biology

Tập 5 Số 8

150041

Thông tin tác giả