Anion-dependent cation transport in erythrocytes
Tóm tắt
A selective survey of the literature reveals at least three major anion-dependent cation transport systems, defined as Na++ Cl-, K++ Cl-and Na++ K++ Cl-respectively. In human red cells, kinetic data on the fraction of K+and Na+influx inhibitable by bumetanide are presented to indicate an Na+:K+stoichiometry of 1:2. For LK sheep red cells the large Cl--dependent K+leak induced by swelling is shown to share many characteristics with that induced by A-ethyl maleimide (NEM) treatment. NEM has complex effects, both inhibiting and then activating Cl “-dependent K+ fluxes dependent on NEM concentration. The alloantibody anti-L can prevent the action of NEM. In human red cells NEM induces a large Cl--dependent specific K+flux, which shows saturation kinetics. Its anion preference is Cl-> Br-> SCN-> I-> NO->3MeSO-4. This transport pathway is not inhibited by oligomycin or SITS, although phloretin and high concentrations of furosemide and bumetanide (over 0.3 mM) do inhibit. Quinine (0.5 mM) is also an inhibitor. It is concluded that at least two distinct Cl--dependent transport pathways for K+are inducible in mammalian red cells, although the evidence for their separation is not absolute.
Từ khóa
Tài liệu tham khảo
Canessa M ., 1980, (N a + K) co-transport an d cell volum e in hum an red blood cells. Fedn. Proc, Biol., 39, 1842
Aiton J ., 1981, O ccurrence of passive furosemide-sensitive transm em brane potassium tran sp o rt in cultured cells. Biochim. biophys, Acta, 646, 389
Aull F., 1981, Potassium chloride cotransport in steady-state ascites tum or cells. Biochim biophys, Acta, 643, 339
Beauge L. A. & Lew V. L. 1977 Passive fluxes of sodium and potassium across cell m em branes. In Membrane transport in red cells (ed. J . C. Ellory & V. L. Lew) pp. 39-51. A cadem ic Press.
Bourne P. K. & Cossin A. R . 1982 O n the instability of K influx in erythrocytes of the rainbow tro u t Salmo gairdari and the role of catecholam ine horm ones in m aintaining in vivo influx activity. J . exp. Biol. (In the press.)
Brading A. F. 1981 Ionic distribution an d m echanism s of transm em brane ion m ovem ents in smooth muscle. In Smooth muscle:an assessment o f current knowledge (ed. E. Biilbring A. F. Brading A. W. Jones & T . T om ita) pp. 65-92. L o n d o n : E dw ard A rnold.
Burg M., 1973, Function of the thick ascending lim b of H en le's loop. Am. J, Physiol., 224, 659
Davson H . 1937 1 he loss of potassium from the erythrocyte in hypotonic saline. J . cell Physiol. 10 247-264.
De Pont J . J . H . H. M. & Bonting S. L. 1981 Anion-sensitive A TPase an d (K+ + H +)-A TPase. In Membrane transport (ed. S. L. Bonting & J . J . H . H . M . de P ont) pp. 209-234. A m sterdam : Elsevier.
Ellory J ., 1981, Passive potassium transport in L K sheep red cells: dependence upon cell volume and chloride. J . Physiol, Lond., 318, 511
D u h m J. 1982 L ithium transport pathw ays in erythrocytes. In Symposium on basic mechanisms in the action o f lithium (ed. H . D. Lux & H . M. E m rich) pp. 1-20. E xcerpta M edica.
D uhm J . Gobeh B. O . Lorenz R. & W eber P. C. 1982 Sodium lithium exchange and sodium potassium cotransport in h um an erythrocytes. P art II. Hypertension 4. (In the press.)
Ellory J . C. & D unham P. B. 1980 V olum e-dependent passive potassium transport in L K sheep red cells. In Membrane transport in erythrocytes (Alfred Benzon Symposium no. 14) (ed. U . Lassen H . H . Ussing & J . O. W ieth) pp. 409-427. C openhagen: M uncksgaard.
Ellory J ., 1982, T h e effect of pressure on passive K+ uptake by hum an erythrocytes and the influence of counter ions. J . Physiol, Pond, 324, 50
Ellory J ., 1981, Glycine transport in hum an erythrocytes. J . Physiol, Lond, 320, 403
Ellory J ., 1982, T he hum an erythrocyte C l"dependent N a -K cotransport system as a possible model for studying the action of loop diuretics. Br, J, Pharmac, 75, 183
Frizzell R ., 1979, Sodium -coupled chloride transport by epithelial tissues, Am. J. Physiol., 236, F1
Funder J . & W ieth J . O. 1980 Passive cation transport by ion p air form ation. In Membrane transport in erythrocytes {Alfred Benzon Symposium no. 14) (ed. U . V. Lassen H . H . Ussing & J . O. W ieth) pp. 520-530. C openhagen: M uncksgaard.
Geek P., 1980, Electrically silent cotransport of Na+, K+ and C l" in Ehrlich cells. Biochim, biophys, Acta, 600, 432
Greger R., 1981, Coupled transport of Na+ and C l" in the thick ascending lim b of H en le's loop of rab b it nephron. Scand. J, Audiol, Suppl, 14, 1
Greger R., 1981, Presence of Ium inal K+, a prerequisite for active N aC l transport in the cortical thick ascending lim b of H enle's loop of rab b it kidney. Pfliigers Arch, Eur, J, Physiol., 392, 92
Jorgensen P. L. 1976 T he function of (N a+ K +)-A TPase in the thick ascending lim b of H en le's loop. In Current problems in clinical biochemistry vol. 6 {Renal metabolism in relation to renal function) (ed. U . Schm idt & U .C . D ubach) pp. 190-199. Berne S tuttgart an d V ienna: H ans H uber.
Ellory J ., 1981, Evidence against N a+-pum p m ediation of C a2+-activated K + transport and diuretic-sensitive (N a + /K +)-cotransport. Biochim. biophys, Acta, 646, 353
Lauf P. K., 1980, A chloride dependent K + flux induced by Y -ethylm aleim ide in genetically low K+ sheep and goat erythrocytes. Biochim. biophys, Res. Commun., 92, 1422
M usch M. W . Field M. & Frizzell R . A. 1982 Active K+ transport by the intestine of the flounder Pseudopleuronectes americanus: evidence for cotransport with N a and Cl. Bull. M D IB L (in the press.)
Rothstein A., 1973, A daptation of mouse leukemic cells (L 5178 Y) to aniosotonic m edia, Expl Cell. Res., 79, 295
Russell J ., 1979, Chloride and sodium influx: a coupled uptake m echanism in the squid giant axon. J . gen, Physiol., 73, 801
Russell J ., 1981, K +-dependent N a + Cl uptake by squid giant axon, Biophys. J., 33, 40a
Russell J ., 1979, Properties of chloride transport in barnacle muscle fibres. J . gen, Physiol., 73, 343
Sachs J ., 1971, O uabain insensitive sodium movements in the hum an red blood cell. J . gen, Physiol., 57, 259
Sachs J ., 1974, A ntibody-induced alterations in the kinetic characteristics of the N a : K pum p in goat red cells. J. gen, Physiol., 63, 389
Sachs J . R . Knauf P. A. & D unham P. B. 1975 T ransport through red cell m em branes. In The red blood cell (ed. D. M. Surgenor) pp. 613-703. Academic Press.
Schultz S. G. 1981 Salt and w ater absorption by m am m alian small intestine. In Physiology o f the gastrointestinal tract (ed. L. R. Johnson) pp. 983-989. New York: Raven Press. 494
Aickin C. C., 1980, J . Physiol, Lond., 300, 40P