Analysis of population genetic structure of Iranian Fusarium oxysporum f. sp. lentis isolates using microsatellite markers

Australasian Plant Pathology - Tập 46 - Trang 35-42 - 2016
Khoshnood Nourollahi1, Mona Madahjalali1
1Plant Protection Department, Ilam University, Ilam, Iran

Tóm tắt

Lentil (Lens culinaris Medik.) is an important widely cultivated food legume crop in Iran. Wilt disease caused by Fusarium oxysporum f.sp. lentis has been observed with high losses in some of cultivated areas during recent years. Simple sequence repeat (SSR) were used to determine the genetic structure, and estimate genetic diversity in 101 of Fusarium oxysporum f. sp. lentis (FOL) isolates from five counties in Ilam provinces of western Iran (Sarableh, Sirvan, Ivan, Badreh, Darehshahr). A set of five microsatellite primer pairs revealed a total of 10 alleles each locus across the five populations; the number of alleles varied from 6 to 9 for each population. A low level of genetic variability was observed among FOL isolates in the region. Genetic diversity was low (H = 0.234) within populations with corresponding high average gene flow and low genetic distances between populations. The smallest genetic distance was observed between isolates from Sirvan and Badreh. Observed allele number (Na) and effective numbers (Ne) of alleles were higher in Ivan in comparison to other populations. The number of (H) and (I) were also higher in Ivan (H = 0.301; I = 0.442). The total gene diversity (Ht) and gene diversities between subpopulation (Hs) were estimated 0.267 and 0.233, respectively. Gene diversity attributable to differentiation among populations (Gst) was 0.126, while gene flow (Nm) was 3.439. Cluster analysis based on UPGMA showed the lowest genetic distance between Sirvan and Badrah, than between Sarableh and Sirvan. The dendrogram indicated a clear break between the population from Darehshahr and the four remaining populations. Results from this study will be useful in breeding for Crown and root rot resistant cultivars and developing necessary control measures.

Tài liệu tham khảo

Abbas A (1995) Variation in some cultural and physiological characters and host/pathogen interaction of Fusarium oxysporum f. sp. lentis, and inheritance of resistance to lentil wilt in Syria. Ph.D. Thesis, Faculty of Agriculture, University of Aleppo, Syria

Abd-Elsalam KA, Omar MR, Migueli Q, Nirenberg HI (2004) Genetic characterization of Fusarium oxysporum f. Sp. vasinfectum isolates by random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). J Plant Dis and Protec 111:534–544

Agrios GN (1997) Plant pathology 4th edition. Academic Press Inc, San Diego

Baayen RP, O'Donnell K, Bonants PJM, Cigelnik E, Kroon LPNM, Roebroeck EJA, Waalwijk C (2000) Gene genealogies and AFLP analyses in the Fusarium oxysporum complex identify monophyletic and nonmonophyletic formae speciales causing wilt and rot disease. Phytopathol 90:891–900

Barnes I, Nakabonge G, Roux J, Wingfield BD, Wingfield MJ (2005) Comparison of populations of the wilt pathogen Ceratocystis albifundus in South Africa and Uganda. Plant Pathol 54:189–195

Barnett HL, Hunter BB (1972) Illustrated genera of imperfect fungi. Burgess, Publication Company, p 241

Barres B, Dutech C, Andrieux A, Caron H, Pinon J, Frey P (2006) Isolation and characterization of 15 microsatellite loci in the poplar rust fungus, Melampsora larici-populina, and cross-amplification in related species. Mol Ecol Not 6:60–64

Barve MP, Haware MP, Sainani MN, Ranjekar PK, Gupta VS (2001) Potential of microsatellites to distinguish four races of Fusarium oxysporum f. sp. ciceri prevalent in India. Theo App Gen 102:138–147

Bayraktar H, Dolar FS (2008) Genetic diversity of wilt and root rot pathogens of chickpea. Turk J Ag 33:1–10

Bayraktar H, Türkkan M, Dolar FS (2010) Characterization of Fusarium oxysporum f. sp. cepae from onion in Turkey based on vegetative compatibility and rDNA RFLP analysis. J Phytopathol 10:1439–0434

Belabid L, Baum M, Fortas Z, Bouzand Z, Eujal I (2004) Pathogenic and genetic characterization of Algerian isolates if Fusarium oxysporum f. sp. lentis by RAPD and AFLP analysis. Af J Biotech 3:25–31

Beniwal SPS, Bayaa B, Weigand S, Makkouk K, Saxena MC (1993) Field guide to lentis diseases and insect pests. International Centre for Agricultural Research in the Dry Areas, Aleppo, p. 106

Bentley S, Pegg KG, Dale JL (1995) Genetic variation among a worldwide collection of isolates of Fusarium oxysporum f. sp. cubense analyzed by RAPD-PCR fingerprinting. Mycol Res 99:1378–1384

Bhalla MK, Nozzolillo C, Schneider E (1992) Observation on the responses of lentil root cells to hypha of Fusarium oxysporum. J Phytopathol 135:335–341

Bogale M, Wingfield BD, Wingfield MJ, Steenkamp ET (2005) Simple sequence repeats markers for species in the Fusarium oxysporum complex. Mol Ecol Notes 5:622–624

Bogale M, Wingfield BD, Wingfield M, Steenkamp ET (2006) Characterization of Fusarium oxysporum isolates from Ethiopia using AFLP, SSR and DNA sequence analyses. Fungal Divers 23:51–66

Britz H, Coutinho TA, Wingfield BD, Wingfield MJ (2002) Sequence characterized amplified polymorphic markers for the pitch canker pathogen Fusarium circinatum. Mol Ecol Notes 3:577–580

Burgess T, Wing M, Wing B (2001) Simple sequence repeat markers distinguish among morphotypes of Sphaeropsis sapinea. Appl Environ Microbiol 67:354–362

Chaudhary RG, Amarjit K (2002) Wilt disease as a cause of shift from lentis cultivation in Sangod tehsil of Kota Rajasthan. Ind J Pulse Res 15:193–194

Clulow SA, Lewis BC, Matthews P (1991) A pathotype classification for Mycosphaerella pinodes. J Phytopathol 131:322–332

Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

Enjalbert J, Duan X, Leconte M, Hovmøller MS, De Vallavielle-Pope C (2005) Genetic evidence of local adaptation of wheat yellow rust (Puccinia striiformis f. sp. tritici) within France. Mol Ecol 14:2065–2073

Groenewald S, Berga NVD, Marasasb WFO, Viljoen A (2006) The application of high-throughput AFLP’s in assessing genetic diversity in Fusarium oxysporum f. sp. cubense. Mycol Res 110:297–305

Haware MP, Nene YL, Natarajan M (1996) Survival of Fusarium oxysporum f. sp. ciceri. Plant Dis 66:809–810

Hawker LE (1950) Physiology of fungi. University of London Press Ltd, London

Jalali BL, Chand H (1992). Disease of cereals and pulses Chickpea wilt Plant disease of International importance Vol I. In: Singh US, Mukhtopadhyay AN, Kumar J, Chaube HS (eds) Prentice Hall Englewood, Cliff NJ pp 420–444

Jarne P, Lagoda PJL (1996) Microsatellites from molecules to populations and back. Trend Ecol Evol 11:424–429

Jens CF, Thrane V, Mathur SB (1991) An illustrated manual on identification of some seed-borne Aspergilli Fusaria Penicillia and their mycotoxins. Danish government institute of seed pathology for developing countries. Ryvans Alle 78 DK 2900 Hellerue Denmark

Jimenez-Gasco MM, Perez-Artes E, Jimenez-Diaz RM (2001) Identification of pathogenic races 0, 1B/C, 5 and 6 of Fusarium oxysporum f. Sp. ciceri with random amplified polymorphic DNA (RAPD). Eur J Plant Pathol 107:237–248

Keller SM, McDermott JM, Pettway RE, Wolfe MS, McDonald BA (1997) Gene flow and sexual reproduction in the wheat glume blotch pathogen Phaeosphaeria nodorum (anamorph Stagonosporanodorum). Phytopathol 87:353-358

Khare MN (1980) Wilt of lentis. Jawaharlal Nehru Krishi Vishwa Vidyalaya Jabalpur MP India, p 155

Kiprop EK, Baudoin JP, Mwang’ombe AW, Kimani PM, Mergeai G, Maquet A (2002) Characterization of Kenyan isolates of Fusarium udum from pigeon pea [Cajanus cajan (L) Millsp] by cultural characteristics aggressiveness and AFLP analysis. J Phytopathol 150:517–525

Kistler HC (2001) Evolution in host specificity in Fusarium oxysporum. In: Summerell BA, Leslie JF, Backhouse D, Bryden WL, Burgess LW (eds) Fusarium. APS Press, St Paul

Kosman E, Leonard J (2005) Similarity coefficients for molecular markers in studies of genetic relationships between individuals for haploid diploid and polyploid species. Mol Ecol 14:415–424

Leslie JF, Summerell BA, Bullock S (2006) The Fusarium laboratory manual. Wiley-Blackwell, New York, p. 388

McDermott JM, McDonald BA (1993) Gene flow in plant pathosystems. Annu Rev Phytopathol 31:353–373

McDonald BA (1997) The population genetic of fungi: tools and techniques. Phytopathol 87:448–453

Migheli Q, González-Candelas L, Dealessi L, Camponogara A, Ramón-Vidal D (1998) Transformants of T. longibrachiatum overexpressing the β-1 4-endoglucanase gene egl1 show enhanced biocontrol of Pythium ultimum on cucumber. Phytopathol 88:673–677

Miller JJ (1946) Taxonomy in Fusarium with particular reference to section Elegans. Can J Res 24:213–223

Mohammadi M, Aminipour M, Banhashemi Z (2004) Isozyme analysis and soluble mycelial protein pattern in Iranian isolates of several formae speciales of Fusarium oxysporum. J Phytopathol 152:267–276

Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325

Mwang Òmbe AW, Thiong G, Olubayo FM, Kiprop EK (2007) DNA microsatellite analysis of Kenyan isolates of Rhizoctonia solani from common bean (Phaseolus vulgaris L.). J Plant Pathol 6:66–71

Nasir M, Hoppe HH (1991) Studies on pathotype differentiation within Mycosphaerella pinodes (Berk, Bloxan) Vestergren a component of the Ascochyta disease complex of peas (Pisum sativum). Zeit Fur Pflam Kran Pflan 98:619–626

Nei M (1973) Analysis of the genetic diversity in subdivided populations. Proc Natl Acad Sci U S A 70:3321–3323

Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. G E N 89:583–590

Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonuclease. Proc Natl Acad Sci U S A 76:5269–5273

Nelson PE, Toussoun TA, Marasas WFO (1983) Fusarium species: An illustrated manual for identification. The Pennsylvania State University Press University Park, p 193

Nourollahi K, Javannikkhah M, Naghavi MR, Lichtenzveig J, Okhovat SM, Richard P, Oliver RP, Ellwood SR (2011) Genetic diversity and population structure of Ascochyta rabiei from the western Iranian Ilam and Kermanshah provinces using MAT and SSR markers. Mycol Prog 10:1–7

O’Donnell K (2000) Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex. Mycol 92:919–938

Ouellet T, Seifert KA (1993) Genetic characterization of Fusarium graminearum strains using RAPD and PCR amplification. Phytopathol 83:1003–1007

Pasquali M, Acquadro A, Balmas V, Migheli Q, Garibaldi A, Gullino ML (2003) RAPD characterization of Fusarium oxysporum isolates pathogenic on Argyranthemum frutescens L. J Phytopathol 151:30–35

Peakall R, Smouse PE (2006) GenAlEx 6: genetic analysis in excel population genetic software for teaching and research. Mol Ecol Notes 6:288–295

Roldan-Ruiz I, Dendauw JE, Vanbockstaele E, Depicker A, Loose M (2000) AFLP markers reveals high polymorphic rates in ryegrasses (Lolium spp.). Mol Breed 6:125–126

Rolhf FJ (1990) NTSYSPc Numerical taxonomy and multivariant analysis system Version 202 Applied Biostatistics. New York

Saharan MS, Naef A (2008) Detection of genetic variation among Indian wheat head scab pathogens (Fusarium spp. /isolates) with microsatellite markers. Crop Protect 27:1148–1154

Sanders IR (2002) Ecology and evolution of multigenomic arbuscular mycorrhizal fungi. Am Nat 160:8–41

Sarker A, Erskine W (2006) Recent progress in the ancient lentil. J Ag Sci 144:19–29

Shah D, Bergstrom GC, Ueng PP (1995) Initiation of septoria nodorum blotch epidemics in winter wheat by seedborne Stagonospora nodorum. Phytopathol 85:452–457

Sirjusingh C, Kohn LM (2001) Characterization of microsatellite in the fungal plant pathogen S. sclerotiorum. Mol Ecol Notes 1:267–269

Sivaramakrishnan S, Kannan S, Singh SD (2002a) Genetic variability of Fusarium wilt pathogen isolates of chickpea (Cicer arietinum L) assessed by molecular markers. Mycopathol 155:171–178

Sivaramakrishnan S, Kannan S, Singh SD (2002b) Detection of genetic variability in Fusarium udum using DNA markers. Ind J Phytopathol 55:258–263

Slippers B, Burgess T, Wingfield BD, Crous PW, Coutinho TA, Wingfield MJ (2004b) Development of simple sequence repeat markers for Botryosphaeria spp. with Fusicoccum anamorphs. Mol Ecol Notes 4:675–677

Sneath PHA, Sokal RR (1973) Numerical taxonomy: the principles and practice of numerical classification. WH Freeman and Company, San Francisco, p. 573

Snyder WC, Hansen HN (1940) The species concept in Fusarium. Am J Bot 27:64–67

Stewart JE, Kim M, James RL, Dumroese RK, Klopfenstein NB (2006) Molecular characterization of Fusarium oxysporum and Fusarium commune isolates from a conifer nursery. Phythopathol 96:1124–1133

Tenzer I, Ivanissevich SD, Morgante M, Gessler C (1999) Identification of microsatellite markers and their application to population genetics of Venturia inaequalis. Phytopathol 89:748–753

Tosi L, Cappelli C (2001) First report of Fusarium oxysporum f. Sp. lentis of lentis in Italy. Plant Dis 85:562

Varshney RK, Chabane K, Hendre PS, Aggarwal RK, Graner A (2007) Comparative assessment of EST-SSR; EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild cultivated and elite barleys. Plant Sci 173:638–649

Welsh J, Petersen C, Mc Clelland M (1991) Polymorphisms generated by arbitrary primed PCR in mouse: application to strain identification and genomic mapping. Nucleic Acids Res 19:303–306

Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphism amplified by arbitrary primers as useful genetic markers. Nucleic Acids Res 18:6531–6535

Woo SL, Zoina A, Sorbo G, Lorito MD, Scala NBF, Noviello C (1996) Characterization of Fusarium oxysporum f. sp. phaseoli by pathogenic races VCGs RFLPs and RAPD. Phytopathol 86:966–973

Yadav SS, McNeil DL, Stevenson PS (2007) Lentis an Ancient Crop for Modern Times. PO Box 17 3300 AA Dordrecht the Netherlands, p 443

Yeh F, Yang R, Boyle T (1999) POPGENE version 131 Microsoft Windows-based software for population genetics analysis. University of Alberta and Centre for International Forestry Research Alberta

Thông tin
Thông tin xuất bản

Australasian Plant Pathology

Tập 46

35-42

Thông tin tác giả