Analysis of biomarker expression in severe endometriosis and determination of possibilities for targeted intraoperative imaging

International Journal of Gynecology & Obstetrics - Tập 121 - Trang 35-40 - 2013
Liseth L. van den Berg1, Lucia M.A. Crane2,3, Marleen van Oosten2,4, Gooitzen M. van Dam2, Arnold H.M. Simons5, H. Sijbrand Hofker2, Joost Bart1
1Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands
2Department of Surgery, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands
3Department of Obstetrics and Gynecology, St. Antonius Hospital, Nieuwegein, The Netherlands
4Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands
5Department of Obstetrics and Gynecology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

Tóm tắt

AbstractObjectiveTo evaluate the expression of biomarkers in endometriotic tissue in order to determine the most promising molecules for targeted intraoperative imaging.MethodsTissue samples were obtained from 18 patients with endometriosis. The intensity and pattern of expression of the following biomarkers were assessed by immunohistochemistry: C‐X‐C chemokine receptor type 4 (CXCR4), epithelial cell adhesion molecule (EpCAM), estrogen receptor (ER), folate receptor α (FR‐α), hypoxia‐inducible factor 1‐α (HIF‐1α), progesterone receptor (PR), and vascular endothelial growth factor A (VEGF‐A). The Target Selection Criteria scoring system was used to select the most promising biomarkers for intraoperative imaging.ResultsExpression of CXCR4, EpCAM, ER, PR, and VEGF‐A was scored as strong in endometriotic epithelium. Expression of FR‐α was detected in 94.4% of samples, whereas HIF‐1α was expressed in just 5.6% of samples. Of note, CXCR4, ER, and VEGF‐A were also expressed in surrounding healthy tissue, thus reducing the target‐to‐background ratio.ConclusionOf the 7 biomarkers assessed in the present study, EpCAM, FR‐α, and VEGF‐A seem the most promising for targeted intraoperative imaging of endometriosis.

Tài liệu tham khảo

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Thông tin xuất bản

International Journal of Gynecology & Obstetrics

Tập 121

35-40

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