Analysis of Arabidopsis with Highly Reduced Levels of Malate and Fumarate Sheds Light on the Role of These Organic Acids as Storage Carbon Molecules

Oxford University Press (OUP) - Tập 152 Số 3 - Trang 1251-1262 - 2010
Martina B. Zell1, Holger Fahnenstich1, Alexandra Maier1, Mariana Saigo1, Elena V. Voznesenskaya1, Gerald E. Edwards1, Carlos S. Andreo1, Frank Schleifenbaum1, Christiane Zell1, Marı́a F. Drincovich1, Verónica G. Maurino1
1Botanisches Institut, Biowissenschaftliches Zentrum, Universität zu Köln, 50674 Cologne, Germany (M.B.Z., H.F., A.M., V.G.M.); Centro de Estudios Fotosintéticos y Bioquímicos, Universidad Nacional de Rosario, Suipacha 531, Rosario, Argentina (M.S., C.A., M.F.D.); Laboratory of Anatomy and Morphology, V.L. Komarov Botanical Institute of Russian Academy of Sciences, 197376 St. Petersburg, Russia (E

Tóm tắt

Abstract While malate and fumarate participate in a multiplicity of pathways in plant metabolism, the function of these organic acids as carbon stores in C3 plants has not been deeply addressed. Here, Arabidopsis (Arabidopsis thaliana) plants overexpressing a maize (Zea mays) plastidic NADP-malic enzyme (MEm plants) were used to analyze the consequences of sustained low malate and fumarate levels on the physiology of this C3 plant. When grown in short days (sd), MEm plants developed a pale-green phenotype with decreased biomass and increased specific leaf area, with thin leaves having lower photosynthetic performance. These features were absent in plants growing in long days. The analysis of metabolite levels of rosettes from transgenic plants indicated similar disturbances in both sd and long days, with very low levels of malate and fumarate. Determinations of the respiratory quotient by the end of the night indicated a shift from carbohydrates to organic acids as the main substrates for respiration in the wild type, while MEm plants use more reduced compounds, like fatty acids and proteins, to fuel respiration. It is concluded that the alterations observed in sd MEm plants are a consequence of impairment in the supply of carbon skeletons during a long dark period. This carbon starvation phenotype observed at the end of the night demonstrates a physiological role of the C4 acids, which may be a constitutive function in plants.

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Oxford University Press (OUP)

Tập 152 Số 3

1251-1262

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