An integrated humoral and cellular response is elicited in pancreatic cancer by α‐enolase, a novel pancreatic ductal adenocarcinoma‐associated antigen

International Journal of Cancer - Tập 125 Số 3 - Trang 639-648 - 2009
Paola Cappello1, Barbara Tomaino, Roberto Chiarle, Paola Ceruti, Anna Novarino, Carlotta Castagnoli, Paola Migliorini, Giovanni Perconti, Agata Giallongo, Michèle Milella, Vladia Monsurrò, Stefano Barbi, Aldo Scarpa, Paola Nisticò, Mirella Giovarelli, Francesco Novelli
1Center for Experimental Research and Medical Studies, San Giovanni Battista Hospital, Torino, Italy.

Tóm tắt

AbstractPancreatic ductal adenocarcinoma (PDAC) is a fatal disease with a very poor 5‐year survival rate. α‐Enolase is a glycolytic enzyme that also acts as a surface plasminogen receptor. We find that it is overexpressed in PDAC and present on the cell surface of PDAC cell lines. The clinical correlation of its expression with tumor status has been reported for lung and hepatocellular carcinoma. We have previously demonstrated that sera from PDAC patients contain IgG autoantibodies to α‐enolase. The present work was intended to assess the ability of α‐enolase to induce antigen‐specific T cell responses. We show that α‐enolase‐pulsed dendritic cells (DC) specifically stimulate healthy autologous T cells to proliferate, secrete IFN‐γ and lyse PDAC cells but not normal cells. In vivo, α‐enolase‐specific T cells inhibited the growth of PDAC cells in immunodeficient mice. In 8 out of 12 PDAC patients with circulating IgG to α‐enolase, the existence of α‐enolase‐specific T cells was also demonstrated. Taken as a whole, these results indicate that α‐enolase elicits a PDAC‐specific, integrated humoral and cellular response. It is thus a promising and clinically relevant molecular target candidate for immunotherapeutic approaches as new adjuvants to conventional treatments in pancreatic cancer. © 2009 UICC

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International Journal of Cancer

Tập 125 Số 3

639-648

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