An integrated confocal and magnetic resonance microscope for observing living cells

D.S. Daly1, R.A. Wind1, P.D. Majors1, K.K. Anderson1, G.R. Holtom1, E.J. Ackerman1
1Pacific Northwest National Laboratory, Richland, WA, USA

Tóm tắt

We have combined a confocal fluorescence optical microscope and a magnetic resonance microscope into one instrument to image simultaneously a living cell system. The high spatial resolution confocal and high spectral resolution magnetic resonance microscope produces a set of closely linked, complementary images. To enhance the value of this image set, we have also developed a statistical method, local linear modeling, to predict a high spatial resolution magnetic resonance image from the confocal optical and magnetic resonance image pair. This high spatial, high spectral resolution image offers insights not available in images obtained from separate studies, nor available in the viewing the image pair as is. This paper presents first results from application of this instrument and local linear modeling, a statistical prediction technique, to a calibration phantom and a Xenopus laevis oocyte cluster.

Từ khóa

#Magnetic resonance #Magnetic force microscopy #Optical microscopy #Spatial resolution #Instruments #Image resolution #Predictive models #Fluorescence #Statistical analysis #Calibration

Tài liệu tham khảo

10.1006/jmre.2000.2212 seber, 1977, Linear Regression Analysis graybill, 1976, Theory and Application of the Linear Model neter, 1974, Applied Linear Statistical Models morrison, 1976, Multivariate Statistical Methods glickson, 2000, Methods in Biomedical Magnetic Resonance Imaging and Spectroscopy, 879 blumler, 1998, Spatially Resolved Magnetic Resonance, Wiley-YCH 10.1016/S0034-4257(97)00090-4 vrabel, 1996, Multispectral Imagery Band Sharpening Study”, Photogrammetric Engineering & Remote Sensing, American Society for Photogrammetry and Remote Sensing, 6209, 1075 10.1016/S0034-4257(99)00074-7 hill, 1999, A Local Correlation Approach for the Fusion of Remote Sensing Data with Different Spatial Resolutions in Forestry Applications, International Archives of Photogrammetry and Remote Sensing, 32, 4 paddock, 1999, Confocal Microscopy. Methods and Protocols 10.1007/978-1-4757-5348-6_19 bretschneider, 2000, Image Fusion in Remote Sensing, Proceedings of the 1st Online Symposium of Electronic Engineers