An in vitro model to evaluate cell adhesion to metals used in implantation shows significant differences between palladium and gold or platinum

Cell Biology International - Tập 28 - Trang 541-547 - 2004
Neill Turner1, Michelle Armitage1, Ronald Butler1, Grenham Ireland1
1Biological Sciences, University of Manchester, M13 9PT, UK

Tóm tắt

AbstractAdhesion of tissue cells to metallic implants is a major factor that is important for proper tissue integration. Adhesion of Swiss mouse 3T3 fibroblasts to gold, platinum and palladium surfaces was investigated. Immunofluorescence staining for the integrin subunits αv and β1 and the focal contact protein vinculin revealed that cells growing on gold and platinum expressed many focal contacts. In contrast, cells on palladium surfaces had reduced numbers of focal contacts shown by vinculin staining and failed to demonstrate expression of αv and β1 in focal contacts. Spread cell area was also significantly reduced on palladium than on other surfaces suggesting that cells on palladium were more weakly attached. This may be due to either a different molecular composition of focal contacts in cells grown on palladium surfaces or unusual microstructural properties of the palladium surface. This model is useful to evaluate adhesion of cells to different metal surfaces.

Tài liệu tham khảo

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Cell Biology International

Tập 28

541-547

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