An enzyme labelled nuclear antigen immunoassay for detection of cytomegalovirus IgM antibodies in human serum: Specific and non‐specific reactions
Tóm tắt
A μ‐capture enzyme linked immunosorbent assay was developed for detection of IgM antibody t o cytomegalovirus (CMV). Virus‐specific IgM was detected using horseradish peroxidase labelled nuclear CMV antigen (CMV‐ELA). False‐positive reactions caused by Paul‐Bunnell‐Davidsohn (PBD) positive sera and antinuclear antibody (ANA) positive sera were identified in a combination assay employing enzyme labelled nuclear control antigen (CO‐ELA) in parallel to the CMV‐ELA. Four of five PBD positive and 30 of 31 ANA positive sera reactive with the CMV‐ELA were identified as false positive reactions in the combined ELA‐assay. The reactivity in PBD‐positive sera could not be explained by antigenic cross reactivity between CMV and Epstein‐Barr virus. and the results further suggested that different cell specified components of the CMV‐ELA were responsible for the reactivity of PBD‐positive as compared to ANA‐positive sera. One of 314 healthy blood donors. 12 of 12 patients with primary CMV infection. and 11 of 15 patients with secondary CMV infection had detectable CMV IgM antibodies. Comparison of different CMV‐ELAs revealed that pronounced differences in specificity as well as sensitivity may exist.
Từ khóa
Tài liệu tham khảo
Cohen IR, 1967, Competition between and effectiveness of IgG and IgM antibodies in indirect fluorescent antibody and other tests, Journal of Immunology, 98, 143, 10.4049/jimmunol.98.1.143
Kangro HO, 1980, Evaluation of a radioimmunoassay for IgM‐class antibodies against cytomegalovirus, British Journal of Experimental Pathology, 61, 512
Mintz L, 1980, Anticomplement immunofluorescence test that uses isolated fibroblast nuclei for detection of antibodies to human cytomegalovirus, Journal of Clinical Microbiology, 12, 562, 10.1128/jcm.12.4.562-565.1980
Rasmussen L, Virus‐specific IgG and IgM antibodies in normal and immunocompromised subjects infected with cytomegalovirus, Journal of Infectious Diseases, 145, 191, 10.1093/infdis/145.2.191
Schmitz H, Detection of immunoglobulin M antibody to Epstein‐Barr virus by use of an enzyme labelled antigen, Journal of Clinical Microbiology, 16, 361, 10.1128/jcm.16.2.361-366.1982
Stagno S, 1978, Use of isolated nuclei in the indirect fluorescent‐antibody test for cytomcgalovirus infection: Comparison with microneutralisation. anticomplement and conventional indirect fluorescent antibody assays, Journal of Clinical Microbiology, 7, 486, 10.1128/jcm.7.5.486-489.1978
Van Loon AM, 1981, Direct enzyme‐linked immunosorbent assay that uses peroxidase‐labelled antigen for determination of immunoglobulin M antibody lo cytomegalovirus, Journal of Clinical Microbiology, 13, 416, 10.1128/jcm.13.3.416-422.1981
Van Loon AM, 1985, Quantitation of immunoglobulin E antibody to cytomegalovirus by antibody capture enzyme‐linked immunosorbent assay, Journal of Clinical Microbiology, 21, 558, 10.1128/jcm.21.4.558-561.1985
Wilson MB, 1978, Immunofluorescense and Related Staining Techniques, 215