An easy “SteamDrop” method for high quality plant chromosome preparation

Claussen U: The pipette method: a new rapid technique for chromosome analysis in prenatal diagnosis. Hum Genet 1980, 54: 277–278. 10.1007/BF00278985

Rǿnne M: Chromosome preparation and high resolution banding (review). J Dairy Sci 1990, 4: 337–365.

Spurberck JL, Zinmeister AR, Meyer KJ, Jalal SM: Dynamics of chromosome spreading. Am J Med Genet 1996, 61: 387–393. 10.1002/(SICI)1096-8628(19960202)61:4<387::AID-AJMG15>3.0.CO;2-O

Hliscs R, Muhlig P, Claussen U: The spreading of metaphases is a slow process which leads to a stretching of chromosomes. Cytogenet Cell Genet 1997, 76: 167–171. 10.1159/000134537

Henegariu O, Heerema NA, Wright LL, Bray-Ward P, Ward DC, Vance GH: Improvements in cytogenetic slide preparation: controlled chromosome spreading, chemical aging and gradual denaturing. Biotech Histochem 2001, 74: 160–166.

Claussen U, Michel S, Mühlig P, Westermann M, Grummt UW, Kromeyer-Hauschild K, Liehr T: Demystifying chromosome preparation and the implications for the concept of chromosome condensation during mitosis. Cytogenet Genome Res 2003,98(2–3):136–146.

Deng W, Tsao SW, Lucas JN, Leung CS, Cheung ALM: A new method for improving metaphase chromosome spreading. Cytometry 2003,51(1):46–51.

Moralli D, Yusuf M, Mandegar M, Khoja S, Monaco ZL, Volpi EV: An improved technique for chromosomal analysis of human ES and iPS cells. Stem Cell Rev 2011, 7: 471–477. 10.1007/s12015-010-9224-4

Belling J: On counting chromosomes in pollen-mother cells. Am Nat 1921, 55: 573–574. 10.1086/279843

Schwarzacher T, Ambros P, Schweizer D: Application of Giemsa banding to orchid karyotype analysis. Plant Syst Evol 1980, 134: 293–297. 10.1007/BF00986805

Schwarzacher T, Leitch AR: Enzymatic treatment of plant material to spread chromosomes for in situ hybridization. Protocols for nucleic acid analysis by nonradioactive probes. Methods Mol Biol 1994, 28: 153–160.

Pijnacker LP, Ferwerda MA: Giemsa C-banding of potato chromosomes. Can J Genet Cytol 1984, 26: 415–419.

Fukui K, Ilijima K: Somatic chromosome map of rice by imaging methods. Theor Appl Genet 1991, 81: 589–596.

Mouras A, Salesses G, Lutz : Sur I’utilisation des protoplastes en cytologie: amelioration d’une methode recente en vue de l’identification des chromosomes mitotiques genres Nzcutiana et Prunus. Caryologia 1978, 31: 117–127. 10.1080/00087114.1978.10796736

Mouras A, Saul MW, Essad S, Potrykus I: Localization by in situ hybridization of a low copy chimaeric resistance gene introduced into plants by direct gene transfer. Mol Gen Genet 1987, 207: 204–209. 10.1007/BF00331579

Murata M: Staining air dried protoplasts for study of plant chromosomes. Stain Technol 1983, 58: 101–106.

Ambros PF, Matzke MA, Matzke AJM: Detection of a 17 kb unique sequence (T-DNA) in plant chromosomes by in situ hybridization. Chromosoma 1986, 94: 11–18. 10.1007/BF00293525

Geber G, Schweizer D: Cytochemical heterochromatin differentiation in Sinapis alba (Cruciferae) using a simple air-drying technique for producing chromosome spreads. Plant Syst Evol 1988, 158: 97–106.

Andras SC, Hartman TPV, Marshall JA, Marchant R, Power JB, Cocking EC, Davey MR: A drop-spreading technique to produce cytoplasm free mitotic preparations from plants with small chromosomes. Chromosome Res 1999, 7: 641–647. 10.1023/A:1009288119851

Kato A, Lamb JC, Birchler JA: Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Proc Natl Acad Sci U S A 2004, 101: 13554–13559. 10.1073/pnas.0403659101

Kato A, Albert PS, Vega JM, Birchler JA: Sensitive fluorescence in situ hybridization signal detection in maize using directly labeled probes produced by high concentration DNA polymerase nick translation. Biotech Histochem 2006,81(2–3):71–78.

Anamthawat-Jonsson K: Preparation of chromosomes from plant leaf meristems for karyotype analysis and in situ hybridization. Methods Cell Sci 2003, 25: 91–95.

Carvalho CR, Saraiva LS: An air drying technique for maize chromosomes without enzymatic maceration. Biotech Histochem 1993, 68: 142–145. 10.3109/10520299309104684

Jewell DC, Islam-Faridi N: A Technique for Somatic Chromosome Preparation and C-Banding of Maize. In The Maize Handbook. Edited by: Freeling M, Walbot V. New York: Springer-Verlag; 1994:484–493.

Rothfels KH, Siminovitch L: An air-drying technique for flattening chromosomes in mammalian cells grown in vitro. Biotech Histochem 1958,33(2):73–77. 10.3109/10520295809111827

Danilova TV, Birchler JA: Integrated cytogenetic map of mitotic metaphase chromosome 9 of maize: resolution, sensitivity, and banding paint development. Chromosoma 2008, 117: 345–356. 10.1007/s00412-008-0151-y

Gill N, Findley S, Walling JG, Hans C, Ma J, Doyle J, Stacey G, Jackson SA: Molecular and chromosomal evidence for allopolyploidy in soybean. Plant Physiol 2009,151(3):1167–1174. 10.1104/pp.109.137935

Findley SD, Cannon S, Varala K, Du J, Ma J, Hudson ME, Birchler JA, Stacey G: A fluorescence in situ hybridization system for karyotyping soybean. Genetics 2010,185(3):727–744. 10.1534/genetics.109.113753

Komuro S, Endo R, Shikata K, Kato A: Genomic and chromosomal distribution patterns of various repeated DNA sequences in wheat revealed by a fluorescence in situ hybridization procedure. Genome 2013,56(3):131–137. 10.1139/gen-2013-0003

Ma Y, Islam-Faridl MN, Crane CF, Stelly DM, Price HJ, Byrne DH: A new procedure to prepare slides of metaphase chromosomes of roses. HortScience 1996, 31: 855–857.

Lawrence GJ, Appels R: Mapping the nucleolus organizer region, seed protein loci and isozyme loci on chromosome 1R in rye. Theor Appl Genet 1986,71(5):742–749.

Alexandrov OS, Divashuk MG, Yakovin NA, Karlov GI: Sex chromosome differentiation in Humulus japonicus Siebold & Zuccarini, 1846 (Cannabaceae) revealed by fluorescence in situ hybridization of subtelomeric repeat. Comp Cytogenet 2012, 6: 239–247.

Rogers SO, Bendich AJ: Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 1985, 5: 69–76. 10.1007/BF00020088

Khrustaleva L, Kik C: Localization of single copy T-DNA insertion in transgenic shallots ( Allium cepa L.) by using ultra-sensitive FISH with tyramide signal amplification. Plant J 2001, 25: 699–707.

Heslop-Harrison JS, Schwarzacher T, Anamthawat-Jdnsson K, Leitch AR, Shi M, Leitch IJ: In-situ hybridization with automated chromosome denaturation. Technique 1991, 3: 109–116.

Schmidt T, Schwarzacher T, Heslop-Harrison JS: Physical mapping of rRNA genes by fluorescent in-situ hybridization and structural analysis of 5S rRNA genes and intergenic spacer sequences in sugar beet ( Beta vulgaris ). Theor Appl Genet 1994,88(6–7):629–636.

Shibata F, Hizume M: Evolution of 5S rDNA units and their chromosomal localization in Allium cepa and Allium schoenoprasum revealed by microdissection and FISH. Theor Appl Genet 2002, 105: 167–172. 10.1007/s00122-002-0950-0

Masamura N, McCallum J, Khrustaleva L, Kenel F, Pither-Joyce M, Shono J, Suzuki G, Mukai Y, Yamauchi N, Shigyo M: Chromosomal organization and sequence diversity of genes encoding lachrymatory factor synthase in Allium cepa L. G3 (Bethesda) 2012,2(6):643–651. 2012

Khrustaleva L, Kirov I, Romanov D, Budylin M, Lapitskaya I, Kiseleva A, Fesenko I, Karlov G: The chromosome organization of genes and some types of extragenic DNA in Allium . Acta Hort 2012, 969: 43–51.

Mosier NS, Sarikaya A, Ladisch CM, Ladisch MR: Characterization of dicarboxylic acids for cellulose hydrolysis. Biotechnol Prog 2001, 17: 474–480. 10.1021/bp010028u

Gallyamov MO, Tartsch B, Khokhlov AR, Sheiko SS, Borner HG, Matyjaszewski K, Moller M: Conformational dynamics of single molecules visualized in real time by scanning force microscopy: macromolecular mobility on a substrate in different vapours. J Microsc 2004, 215: 245–256. 10.1111/j.0022-2720.2004.01381.x

Greilhuber J: Why plant chromosomes do not show G-bands? Theor Appl Genet 1977, 50: 121–124.