An allelic discrimination SNP assay for distinguishing the mitochondrial lineages of European wildcats and domestic cats

Conservation Genetics Resources - Tập 4 - Trang 163-165 - 2011
Ross McEwing1, Andrew C. Kitchener2, Clare Holleley3, Kerry Kilshaw4, Paul O’Donoghue5
1WildGenes Laboratory, Royal Zoological Society of Scotland, Edinburgh, Scotland, UK
2Department of Natural Sciences, National Museum of Scotland, Edinburgh, Scotland, UK
3Laboratory of Genomic Diversity, National Cancer Institute, Frederick, USA
4Department of Zoology, Wildlife Conservation Research Unit, University of Oxford, Oxfordshire, UK
5Department of Biological Sciences, University of Chester, Chester, UK

Tóm tắt

Here we present an allelic discrimination assay designed to distinguish European wildcat mtDNA lineages from those of the domestic cat. Introgression between the native wildcat and introduced domestic cat has the potential to limit and reduce the recent recovery of remnant populations. Applied conservation genetic techniques can aid current conservation decisions on lethal control and neutering measures in hybrid zones. This real-time PCR technique offers a rapid, inexpensive and reliable assay to assess mtDNA introgression in the wild and has already identified hybrid individuals in the Scottish wildcat captive breeding programme.

Tài liệu tham khảo

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Conservation Genetics Resources

Tập 4

163-165

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