An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain

Scientific Reports - Tập 7 Số 1
Wei‐Jie He1, Limin Zhang2, Shuyuan Yi1, Xue-Ling Tang1, Qing‐Song Yuan1, Mao‐Wei Guo1, Aibo Wu1, Bo Qu1, He‐Ping Li3, Yu‐Cai Liao4
1Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, 430070, China
2State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan, 430071, China
3College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
4College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Tóm tắt

AbstractDegradation of toxins by microorganisms is a promising approach for detoxification of agricultural products. Here, a bacterial strain, Sphingomonas S3-4, that has the ability to degrade the mycotoxin deoxynivalenol (DON) was isolated from wheat fields. Incubation of Fusarium-infected wheat grains with S3-4 completely eliminated DON. In S3-4 DON is catabolized into compounds with no detectable phytotoxicity, 3-oxo-DON and 3-epi-DON, via two sequential reactions. Comparative analysis of genome sequences from two DON-degrading strains, S3-4 and Devosia D17, and one non-DON-degrading strain, Sphingobium S26, combined with functional screening of a S3-4 genomic BAC library led to the discovery that a novel aldo/keto reductase superfamily member, AKR18A1, is responsible for oxidation of DON into 3-oxo-DON. DON-degrading activity is completely abolished in a mutant S3-4 strain where the AKR18A1 gene is disrupted. Recombinant AKR18A1 protein expressed in Escherichia coli catalyzed the reversible oxidation/reduction of DON at a wide range of pH values (7.5 to 11) and temperatures (10 to 50 °C). The S3-4 strain and recombinant AKR18A1 also catabolized zearalenone and the aldehydes glyoxal and methyglyoxal. The S3-4 strain and the AKR18A1 gene are promising agents for the control of Fusarium pathogens and detoxification of mycotoxins in plants and in food/feed products.

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